Keep us updated, and a bioassay. That teal staining I don't fully understand yet, but the most potent classic cube I've grown had green staining, even in the hyphal knotting.
I haven't been able to duplicate yet, don't know if it was genetic, but I fear it was caused by contaminant stress.
I'll do what I can to give a history of this grow. It started out as a syringe (don't remember the vendor but could be Ralphstersspores about year and a half old) that I put to agar. I sampled it a couple times, the second showing good rhizomorphic development so I did a third sample for good measure then put it to LC. I let it gestate for a couple weeks before putting it to 3 quarts rye, 8cc each. I didn't test the LC as it didn't show any signs of contams, no cloudiness except for the mycelia settling to the bottom, off color solution or floaters. I let the rye colonize to 25% (or so) then did one shake. As usual it colonized nicely except for the bottom area at which time I gently moved the mass towards the lid and left it upside down for a week or so to let it finish colonizing that area. It seems to help if I unscrew the lid (plastic) a quarter turn to help with GE. I've moved away from the separate lid and hoop for that reason. I've got temp controlled seedling mats that I do all my colonizing/agar/fruiting work on keeping it at 75 F or so depending on what I'm working on.
I use a manure based sub of a 2:1:1 mix of manure, verm, coir in that order along with gypsum, coffee grounds and azomite. For the first time I used 1/4" hardware cloth to screen the manure, I had no idea there was so much wood and stones in there to hide contams. The azomite is an organic mix of trace elements, good or bad it's something I learned from a video. I obtained an electric slow roaster that I do a double boiler method for pasteurization, much better results than oven pasteurization. The spawn to sub ratio was 1:2. One day later I observed hyphal knots poking through the sub and by day 4 the surface was covered. I kept the temp around 78. I could have put it to FC then but let it go to day 9 (Dec 28) before doing that. At that point I started misting and fanning the tub sides and lid 3x a day but the surface got matted and bruised with all the moisture so I increased the fanning to 90 seconds, that helped dry the surface at which point I backed off on the duration. Five days later (Jan 2) I observed primordia on the surface. I think the first pins started showing up two days later. Six days later the harvesting started then ending two days later with a few shrooms showing signs of cracking on the caps.Total harvest for the first flush, 287.76 g wet. With the cracking and the tub feeling light I decided to dunk it for three hours, drained for 1 hr and fanned for another hour to dry off the surface. Probably too much fanning but the surface was seriously blue from bruising. This morning it seems to be recovering.
I used a flash for the pic and it washed out some of the teal color on the shrooms. Sorry, I forgot to add a lighter for context but I'm happy with the size, especially compared to the Treasure Coast from a few months ago. Those were embarrassingly small...
After dehydrating them the total yield for the first flush is 42.35 g. The next decision is whether to separate the caps from stems or leave it as is. There is some evidence on Reddit that each has a somewhat different effect, the caps being more visual and the stems having more of an effect on body load. I can do without the body load... Time for a trial run.
Edit: While misting/fanning the tubs I found this in the Amazon:
Mold? contam of some sort?
This looks like more primordia in the making:
Edited by FunnyFarmer, 13 January 2022 - 03:21 PM.