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Does this look like Psilocybe allenii mycelium to you?


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#1 orangutan

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Posted 09 December 2021 - 10:16 AM

I received a handful of semi-colonized woodchips from a landscape border in an apartment complex that had Psilocybe alleniis growing out of it.

 

This is the growth that I'm getting.  I'm almost certain it's the allenii, but how do I know it's not some other random mushroom or mold?  

 

What do yall think?

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#2 HrVanker

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Posted 09 December 2021 - 10:34 AM

It's difficult to know if you don't fruit them. Perhaps an Allenii grower will chime in, but you're going to have to grow them out to be certain.

How positive was the ID on the fruits growing in the bed? I would have started w/ a spore print just be sure.
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#3 orangutan

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Posted 09 December 2021 - 11:16 AM

The ID on the fruits was very positive, they tasted very good.


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#4 MushLuvR

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Posted 09 December 2021 - 12:24 PM

I remember your other post, that looks GREAT man Congrats.  Nice Rhizo's FO' SHO'   :meditate:


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#5 Mycol

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Posted 09 December 2021 - 01:31 PM

Eating the bag !
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#6 YoshiTrainer

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Posted 09 December 2021 - 04:25 PM

That is pretty awesome Orangutan! Not much help but here is Allenii jumping from agar to fortified sawdust. Nice white myc and did form rhizo growth in a previous bag, like your myc.

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#7 rockyfungus

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Posted 09 December 2021 - 11:25 PM

Orang: It's a wood lover alright. In my limited experience with a ton of different outdoor guys on chips only the psilocybes throw those rhizo. Gourmets are a bit different (fluffier) and never come across other woodies here.

 

Yoshi: Loving the sawdust to agar. Next step is smooshing that cake into more wood haha. 

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  • KO.JPG

Edited by rockyfungus, 09 December 2021 - 11:28 PM.

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#8 YoshiTrainer

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Posted 10 December 2021 - 12:05 AM

Yoshi: Loving the sawdust to agar. Next step is smooshing that cake into more wood haha.


That is my hope!
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#9 rockyfungus

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Posted 10 December 2021 - 08:00 AM

 

Yoshi: Loving the sawdust to agar. Next step is smooshing that cake into more wood haha.


That is my hope!

 

It's interesting if that jar was grain, I'd think you're going to fail.

With sawdust or wood my gourmets and actives do some wild stuff and almost feel easier then other species to get colonization through bad habits or silly gambles.


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#10 Mycol

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Posted 10 December 2021 - 08:09 AM

Give me a break, give me a break
Break me off a piece of that All En Nii
:P

Edited by Mycol, 10 December 2021 - 02:40 PM.

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#11 orangutan

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Posted 12 December 2021 - 07:31 PM

In another group I came across a post where Alan Rockefeller says that you can add Ehrlich reagent to mycelium to see if it’s from an active species. There will be trace amounts of indoles in Psilocybe mycelium (obviously less than in a fruit) and it will turn purple with Ehrlich reagent. So I got some mycelium from my wood chips and also some random mycelium from outside (as a control) and did a little study. I can confirm this is allenii mycelium. It changed color when treated with Ehrlich reagent.

1) random mycelium
2) random mycelium + Ehrlich 1 minute

IMG_3180.jpeg IMG_3181.jpeg

3) allenii (?) mycelium
4) allenii (?) mycelium + Ehrlich 1 minute

IMG_3182.jpeg IMG_3183.jpeg

5) random mycelium + Ehrlich 45 minutes
6) allenii (?) mycelium + Ehrlich 45 minutes

IMG_3188.jpeg IMG_3189.jpeg

 

You can see that the random mycelium never changed color.  

And the alleged allenii mycelium went from pure white to grayish and the reagent it's floating in has a slight purple color, seen here when you compare 4 to 6.

IMG_3183.jpeg IMG_3189.jpeg

 


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#12 YoshiTrainer

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Posted 13 December 2021 - 12:04 AM

That is an interesting tidbit of information about the Ehrlich reagent.

Time to start spreading that bark all over your place!

Edited by YoshiTrainer, 13 December 2021 - 12:05 AM.

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#13 rockyfungus

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Posted 13 December 2021 - 12:17 AM

I had a strong feeling that anything rhizoing out is likely to be psilocybe. I just hate blanket it or ALWAYS statements.
I really need to get some Ehrlic Reagent. You could theoretically use it with a gradient to determine potency?


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#14 HrVanker

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Posted 13 December 2021 - 02:31 PM

I've honestly had some doubts about Ehrlich's reagent (at least on its own) for a while now. Sure It's better to test drugs than not, but indole molecules are pretty common and easy to find. I'm not sure how viable it would be, but one could add some tryptophan or 5-HTP while laying RCs to get false Ehrlich's positives.

Similarly, fungi and plants can (and do) produce serotonin and other indole compounds that would also react w/ Ehrlich's, thus giving a positive result.

I'd love to see if our more chemistry inclined folk (@Phineas_Carmichael or @Norman) have any light to shed on my suspicions, and could perhaps assuage them.

Edited by HrVanker, 13 December 2021 - 02:35 PM.

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#15 YoshiTrainer

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Posted 14 December 2021 - 12:38 AM

Orangutan, these photos popped up in the gallery.

https://mycotopia.ne...-cyanofriscosa/

 https://mycotopia.ne...plant-fruiting/

 https://mycotopia.ne...retty-mycelium/

Edited by YoshiTrainer, 14 December 2021 - 12:43 AM.

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#16 orangutan

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Posted 14 December 2021 - 10:28 AM

@HrVanker, those are some very good points.

 

For my needs, the Ehrlich reagent is good enough.  I am looking at some very rhizomorphic mycelium that is growing out of wood chips that were taken from the immediate vicinity of a cluster of allenii fruits.  (I've confirmed they are allenii by eating them).

 

The likelihood that this mycelium is some other fungus is already quite low... and now with this test... the likelihood that this mycelium is some other fungus that ALSO produces indoles is so infinitesimal that I am willing to take the chance and invest the time an effort to cultivate it in an outdoor bed.  

 

@Yoshi, thanks for the photos!  That rhizomorhpic mycelium looks very familiar!


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#17 Arathu

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Posted 14 December 2021 - 09:34 PM

I'd be expanding those chips in prep for a bed and a tub......just to see what comes of them.....

 

It looks promising enough to me that I'd grow them out....

 

Good stuff....can't wait to see some woodies popping.....

 

A


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#18 rockyfungus

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Posted 15 December 2021 - 06:07 PM

There's too many fungi to know what the chips are.

Here's what I know in my backyard. Time will tell as I made these beds. No fruits yet as there's way too much food!

Enoki

enoki.JPG

Wine Cap

wc.JPG

Ko in a bag on wood chips really mature but no pins

Ko.JPG

Ko outdoors

KOout.JPG

Then your wood loving psilocybes

cyan.JPG

 


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#19 orangutan

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Posted 15 December 2021 - 11:06 PM

thank you for the mycelium porn @Rocky






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