Playing with agar again
Posted 20 December 2021 - 11:52 PM
I've been getting a bit jealous of some of these flow hood builds lately but still don't have a dedicated space for one. Maybe I can start scavenging for parts over the winter. But for now. Let's work with what we got.
My first new flavor is gonna be mexicana A. I know a few of you got the same one. Thanks Don. I tried some tampanensis last year, but didn't get great results. I've got more of that same genetics and I'm hoping to have a ghetto fabulous Martha constructed soon to give them a different fruiting condition.
Enough jibber jabber. Here's my first agar experiment for this year.
Mexicana A strain.
On MEA no pour
On bird seed grain water
There's not much to see yet. It's only been a couple days in a cold garage. But I had 3 different agar mixes left so I tried a little of each. Looks like the PDA is starting to fuzz up first. And I kind of like this comparison germination just to see what happens.
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Posted 21 December 2021 - 05:10 AM
Sprinkle and sprout.....there's fungus amongus.....
I like to use a streaking pattern so the odds become much better............ that I'm expanding what intended to grow
I have plans to set up a nice incubator to keep my plates and other cultures in.......
I'm going on vacation (staying away from work before they take my vacation days) over the holiday......I'll probably pour some plates....
I have a few cultures that need to be revived and expanded....good stuff man
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Posted 23 December 2021 - 01:02 PM
MEA still not taking off
Grain water showing a bit
PDA still in the lead.
This one has a little slime at the edge.
And I transferred a piece from a dried up Cambodian isolate while I had the SAB out. It looks to be coming back a little.
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Posted 23 December 2021 - 01:52 PM
Looking Good in Here... Agar has it's Own rewards... fun to play with and isolate, or just clean up and make sure your culture is CLEAN. Nice Job!
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Posted 23 December 2021 - 04:00 PM
Agar is definitely the bomb...... transfer away from what ever ails ya.....
Stick the transfer to the wall of a inverted plate close to but not touching the new agar surface.....let some mycelium make the leap itself and then continue transfers......
There's demonstrations all over Topia of many different techniques that work.....don't hesitate to try simple brown rice flour agar....can't see through it too well but that didn't stop me...
So many things we can do this way....very cool....maybe they'll give me another stimulus check and I'll get an incubator......hah
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Posted 26 December 2021 - 02:21 PM
MEA and Grain water are a bit slower.
The PDA is almost ready to pick a couple transfers before they grow into each other.
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Posted 29 December 2021 - 03:41 PM
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Posted 02 January 2022 - 02:12 PM
how'd ya do the grain water?
I just strained it and filled my 500ml bottle after cooking grain for jars. Then used the same amount of agar powder as usual.
Had to try it after you were talking about it some time back. After seeing the side by side comparisons I might add a little bit of nutritional yeast or sugar on the next try. The straight grain water seems to run a lot slower. But it still works.
Posted 02 January 2022 - 03:27 PM
Posted 18 March 2022 - 06:24 PM
Zipped down and got good stuff to grab.
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Posted 25 March 2022 - 12:48 PM
Last night I looked and happy to see it fuzzing up a bit. Now I just gotta hope it will like the RGS agar
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Posted 31 March 2022 - 10:28 PM
the revived Tampa Plares look good .!
Is your next steps a few RGS jars for stones and few jars for Spawn for fruits ?
Oh and some LC ,?
Good Luck with conntinued succes with reviving The old dried out Tamp plate . ,
it will be interesting to compare to the MEX-A strain .with this strain of Tamp Awsome