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Playing with agar again


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#1 Oldpunk

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Posted 20 December 2021 - 11:52 PM

Hey y'all . been pretty busy but I think it's about time to start some new projects. I have been here for just about a year now and have learned so much....and yet barely scratched the surface. So I dug out last years agar supplies and figure I will start playing around and try to learn some new tricks.

I've been getting a bit jealous of some of these flow hood builds lately but still don't have a dedicated space for one. Maybe I can start scavenging for parts over the winter. But for now. Let's work with what we got.

My first new flavor is gonna be mexicana A. I know a few of you got the same one. Thanks Don. I tried some tampanensis last year, but didn't get great results. I've got more of that same genetics and I'm hoping to have a ghetto fabulous Martha constructed soon to give them a different fruiting condition.

Enough jibber jabber. Here's my first agar experiment for this year.

Mexicana A strain.

On MEA no pour

20211220_211913~2.jpg

On bird seed grain water

20211220_211816.jpg

On PDA

20211220_211743.jpg

There's not much to see yet. It's only been a couple days in a cold garage. But I had 3 different agar mixes left so I tried a little of each. Looks like the PDA is starting to fuzz up first. And I kind of like this comparison germination just to see what happens.
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#2 Arathu

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Posted 21 December 2021 - 05:10 AM

Sprinkle and sprout.....there's fungus amongus.....

 

I like to use a streaking pattern so the odds become much better............ that I'm expanding what intended to grow

 

I have plans to set up a nice incubator to keep my plates and other cultures in.......

 

I'm going on vacation (staying away from work before they take my vacation days) over the holiday......I'll probably pour some plates....

 

I have a few cultures that need to be revived and expanded....good stuff man

 

A

 

.


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#3 Oldpunk

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Posted 23 December 2021 - 01:02 PM

A few days later.....

MEA still not taking off
20211223_105701~2.jpg

Grain water showing a bit
20211223_105414.jpg

PDA still in the lead.
20211223_105335.jpg
This one has a little slime at the edge.
20211223_105453.jpg

And I transferred a piece from a dried up Cambodian isolate while I had the SAB out. It looks to be coming back a little.
20211223_105601.jpg
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#4 MushLuvR

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Posted 23 December 2021 - 01:52 PM

Looking Good in Here... Agar has it's Own rewards... fun to play with and isolate, or just clean up and make sure your culture is CLEAN.  Nice Job!


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#5 Arathu

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Posted 23 December 2021 - 04:00 PM

Agar is definitely the bomb...... transfer away from what ever ails ya.....

 

Stick the transfer to the wall of a inverted plate close to but not touching the new agar surface.....let some mycelium make the leap itself and then continue transfers......

 

There's demonstrations all over Topia of many different techniques that work.....don't hesitate to try simple brown rice flour agar....can't see through it too well but that didn't stop me...

 

So many things we can do this way....very cool....maybe they'll give me another stimulus check and I'll get an incubator......hah

 

A


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#6 Oldpunk

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Posted 26 December 2021 - 02:21 PM

Whole lotta blurry pics . Not sure why I couldn't get to focus. But it looks like I've got plenty of options.

MEA and Grain water are a bit slower.
20211225_211912.jpg 20211225_211602.jpg

The PDA is almost ready to pick a couple transfers before they grow into each other.
20211225_211649.jpg
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#7 Oldpunk

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Posted 29 December 2021 - 03:41 PM

MEA blew up .

20211228_184528.jpg 20211228_184500.jpg

Grain water getting there.

20211228_184256.jpg

And PDA is ready to transfer I think.

20211228_184119.jpg
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#8 rockyfungus

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Posted 29 December 2021 - 06:11 PM

how'd ya do the grain water?



#9 Oldpunk

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Posted 02 January 2022 - 02:12 PM

how'd ya do the grain water?


I just strained it and filled my 500ml bottle after cooking grain for jars. Then used the same amount of agar powder as usual.
Had to try it after you were talking about it some time back. After seeing the side by side comparisons I might add a little bit of nutritional yeast or sugar on the next try. The straight grain water seems to run a lot slower. But it still works.

#10 rockyfungus

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Posted 02 January 2022 - 03:27 PM

You need to dilute it down. It’s most likely too nutritious. Yeast is great additive. Little goes a long way.

#11 Oldpunk

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Posted 05 January 2022 - 10:10 PM

T1 cups of Mex A

20220105_203404.jpg 20220105_203322.jpg 20220105_203234.jpg

I used the MEA and one grain water germ plates to start some MS grain jars.
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#12 Oldpunk

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Posted 05 January 2022 - 10:13 PM

And some Cambodian reaching out from the agar.

20220105_202856.jpg
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#13 ElPirana

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Posted 06 January 2022 - 07:35 AM

Looking good Oldpunk! I love working with agar.

#14 Arathu

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Posted 06 January 2022 - 07:13 PM

Look.....fuzzy wedges! 

 

A


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#15 Oldpunk

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Posted 16 February 2022 - 12:09 AM

Took a few minutes to transfer agar to grain tonight. Always cool to open up my box to some nice specimens.

Mexicana A
20220215_220641.jpg
Cambodian
20220215_220607.jpg
And a nice even Golden Teacher clone
20220215_220539.jpg
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#16 Oldpunk

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Posted 18 March 2022 - 05:00 PM

I was playing with the agar again and noticed how badly I flopped the wedge around last time .

It's kinda cool lookin though.

IMG_20220318_161019~2.jpg
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#17 rockyfungus

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Posted 18 March 2022 - 06:24 PM

Zipped down and got good stuff to grab. 


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#18 Oldpunk

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Posted 25 March 2022 - 12:48 PM

So I decided to try making rye grass seed agar last week. And I've put a couple transfers to it but not enough time has passed to see if they're happy with it. But this one is cool. I made a Tampa LC over a yr ago and did not refrigerate it. Now I'm getting no growth whatsoever.....i thought I had lost the genetics which sucks. I found a year old dried up Tampa agar plate and decided to try it.
Last night I looked and happy to see it fuzzing up a bit. Now I just gotta hope it will like the RGS agar

IMG_20220324_144021.jpg
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#19 Oldpunk

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Posted 31 March 2022 - 07:56 PM

Checked out the RGS agar and I'd have to say the mycellium is all right with it.

Cambodian
20220331_181757.jpg

Year old Tampanensis
20220331_182132.jpg

And a good example of a good sample contaminated
20220331_182242.jpg
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#20 bezevo

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Posted 31 March 2022 - 10:28 PM

the revived Tampa Plares look good .!

 

Is your next  steps  a few RGS  jars for stones and few jars for Spawn for fruits ?

Oh and some LC ,?

 

Good Luck with conntinued succes with reviving The old dried out Tamp plate .  ,

 

it will be interesting to compare to the MEX-A strain .with this strain of Tamp Awsome  

 

bez  !






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