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5 year old m.s. syringe on agar.


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#1 CycleSam

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Posted 27 July 2022 - 02:48 PM

Hello,

Need advice:

Starting back up again after 4 years.
My spore prints: Pan Cayan and Atl. 7 started to germinate after 6 days. Good to go spores.

I also used 5 year old spores I had left over on the same day. 7/21
It's been over 4 weeks and I can still see the spores on the agar. No signs of life.

Any suggestions?

I don't know if I should just toss the Old syringes or wait another few weeks to see if they germinate.

Thank you for any advice.
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#2 MushLuvR

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Posted 27 July 2022 - 04:55 PM

The longest i have went saving a syringe is about 3.5 years, I managed to get a tiny spot of growth on grain actually (shot up the jar twice), I went Agar and grain to test.  That being said, the other 4 syringes I had forgotten about didn't survive.  Prints can be saved for many years if taken care of.  Good Luck.

 

MushL


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#3 YoshiTrainer

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Posted 27 July 2022 - 05:01 PM

So long as the agar is still clean, you don't have much to loose by waiting. 4 weeks does seem like a long time though. Maybe give the syringe a solid shaking and try again if you have extra agar?

Congrats on the Pan and Alt7!
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#4 CycleSam

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Posted 30 July 2022 - 06:05 AM

I really thought the spores in the syringe would still be active.

I'm glad I have prints left over from my past endeavors.

I can seen the Pan Cayan spores still sitting on the agar.

Never knew there was a shelf life MS syringes they look good but definitely no sings of life.

Glad to be back here again.

Have a good day folks.
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