Been upping my skill level in the agar game! Woo! Still no expert but I'm it's becoming more routine
However, I'm finding the continual transfer of contamination- specifically bacterial- when transferring between plates to be irksome- so I'm investigating the use of antibacterial agents in the agar mix.
I'm wondering about your experience here- I've read that many folk use gentamyacin- how much do you add, and in what form?
Edit: according to info found in this thread https://mycotopia.ne...antibiotic-agar
It's gentamiacin sulfate which is added to the agar prior to sterilization because it can withstand pc temps. And, an internet search , turned up 25g for 200$ ... I'm imagining that means it's likely the other internet search that turned up 250 g of Gentamicina Oxitetraciclina in powder form for agricultural use for about 20 dollars - is probably not going to be effective- but hey at that price an experiment may be worth it.
Also, are there downsides from the perspective of colony health when using antibiotics?
Edit: Coors Mikey - No matter what though when I use antibiotic agar I always notice slower germination times.
First photo- an example of the type of contamination which most commonly enters/contaminates my blank plates during pouring
Another contam photo- this is transfer #4 from a clone of gt-. First time ive ever seen that red I think
Edited by Severian, 23 November 2022 - 11:02 AM.