Reasons for slow colonization( on grain?)
Posted 18 January 2023 - 03:50 PM
However, I went agar to grain on the 11th, and still the mycellium is juuust beginning to climb into the area immediately around the wedge-
At first I didn't shake up the jar, so the wedge was face down on all of the grain, so maybe that amount of air was affecting it somehow?
The hydration shouldn't be an issue, I used Yoshi's no prep tek- with rye- 190 g rye to 200 g water ,PC for 90 mins at 15 psi.
I was expecting much faster growth given the healthy look of the mycellium.
None of the jars show any visible sign of contamination.
Any ideas? Or maybe this is just a slow culture?
Posted 19 January 2023 - 03:57 AM
What kind of mushroom is it?
Did you have any problems growing it out on agar?
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Posted 19 January 2023 - 10:13 AM
I'm growing golden teacher, from a clone I took of a mush fruiting outdoors from a spent-sub pile-
On agar, it appears to have some resistance to green mold and some other contams cuz I've seen it eat through them.
I did the first tissue sample last month, so the culture has been on agar for a month - maybe it needs some time remembering! But rye was the grain originally used for spawn so that memory should be in there somewhere
Posted 19 January 2023 - 01:52 PM
Whip up a new batch of grains an try again thats really all you can do if they arent colonizing you current grains, do you have any pics by chance?
And no carb agar sounds like a bold move, cubensis typically like carbs. Is there a reason you opted for this route?
You really cant go wrong with potato dextrose agar its pretty easy to make.
Edited by DocOct, 19 January 2023 - 01:59 PM.
Posted 19 January 2023 - 02:44 PM
Photos to come later
Posted 19 January 2023 - 05:14 PM
My guess would also be that it's probably taking a while to adapt to a new food source, assuming no currently-invisible contamination. If that's the case it will pick up the pace at some point (or else contamination will reveal itself).
Do you have several jars going that were inoculated from the same plate?
If so then they should all be doing the same thing if it's a matter of re-tooling the digestive enzyme(s). It would be uniquely bizarre if multiple jars were all contaminated with something invisible, but nature has no problem being uniquely bizarre so it can't be ruled out entirely yet.
Either way it looks like it's a "wait and see" kind of problem since messing with inoculated jars is generally a bad idea.
Posted 19 January 2023 - 06:26 PM
Ya all from the same plate and now I am seeing contamination- curious though that the slow growth started immediately...
Ahh though I think I'm trying too hard to point the finger at a single 'cause'...
Both 'taking time to change food source'
Thus giving the small amount of contam spores time to get ahold.
Posted 21 January 2023 - 11:46 AM
'mycellium grown on the same agar media for an extended period of time can become media dependent- losing their ability to digest other media, thus the reason for additives- yeast, peptone etc'
I would have thought this only occured over 'an extended period of time', but perhaps a month / 5 transfers is considered an extended period.