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Self Healing Injection Port Jar Lids W/ Waterproof bandaids


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#61 zeegos

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Posted 22 November 2006 - 03:53 PM

talking about tyvek... iv just bought an XL disposable tyvek overall suit for £3, anyone heard or used tyvek from this source? or maybe know if its decent stuff?
its been 2 weeks and this joker on ebay still hasnt sent me it :thumbdown:

#62 Doctor D

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Posted 22 November 2006 - 05:57 PM

Not trying to diss your innovation, Indicaz, as I realize there are many ways to skin a cat and it looks like your cat is skinned proper.

But can't you just as easily omit the hole for air exchange with two self-healing ports on the jar lid, and then fill the LC jar only half way to leave room for air? I've followed BuckarooBanzai's teks for self-healing injection ports and LCs with 100% success so far.

Again, I mean no disrespect to your obviously successful method. I'd just like to know what you feel the advantages are to having an air filter on your LC jars as opposed to not having one at all.


http://mycotopia.net...ead.php?t=13806

http://mycotopia.net...ead.php?t=13801

Make at least two lids for liquid culture jars. liquid culture jars don’t need tyvek vents, just two silicone injection ports.



#63 wtoad

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Posted 22 November 2006 - 06:11 PM

so i successfully made a great looking brazilian lc with these lids indicaz.

only problem was, when i pulled my soccorex tube out of the jar, there was a crazy vaccum. Seems the filters dont breathe worth shit...

anyone else with this problem?


I had this problem too. But with good growth. Seems maybe mushies don't require as much air exchange as we think they do?

#64 Guest_indicaz_*

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Posted 23 November 2006 - 12:14 AM

Not trying to diss your innovation, Indicaz, as I realize there are many ways to skin a cat and it looks like your cat is skinned proper.

But can't you just as easily omit the hole for air exchange with two self-healing ports on the jar lid, and then fill the LC jar only half way to leave room for air? I've followed BuckarooBanzai's teks for self-healing injection ports and LCs with 100% success so far.

Again, I mean no disrespect to your obviously successful method. I'd just like to know what you feel the advantages are to having an air filter on your LC jars as opposed to not having one at all.


http://mycotopia.net...ead.php?t=13806

http://mycotopia.net...ead.php?t=13801



you could and im sure you wouldnt have a problem... unless however that lc was to be stored for awhile, but i cant say im not a expert...

in that case clean filtered gaseous exchange misgh help sustain that culture? Stagnant air that has no exchange can eventually lead to contamination? I dont know lol

I really cant say if any works better or worse.... who cares really... this is what i found that has worked for me... I know what BB has works for him as well... There is no exact science here....

#65 Hippie3

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Posted 23 November 2006 - 10:21 AM

Now someone needs to figure out how to make the lids not rust

maybe a clear laquer? that can withstand heat and water/fluids....


look into that


peace
indi


i just put a blob of RTV silicon under the lid holes, as well as above.
no rust.

#66 Laffyn

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Posted 12 December 2006 - 02:16 PM

Will the self healing injection ports work with BRF TEK?

I'm having a difficult time reducing contams during innoculation.

#67 boomer1400

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Posted 12 December 2006 - 02:35 PM

Will the self healing injection ports work with BRF TEK?

I'm having a difficult time reducing contams during innoculation.


Yes sir. It will work great.

#68 r00kn00b

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Posted 13 December 2006 - 05:16 PM

I have made a batch of these lids and PC'ed them the other day. Now that they've cooled, I was wondering how much effort I need to put into sterility when i noc the jars up. I don't live alone and previously I've used the bathroom and removed everything and lysol bombed as well as used a HEPA filter in there. I didn't have any contam problems with that method. However, it's not easy for me to go to all of that trouble and be unnoticed. Would I be ok if i just flame the needle and such?

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#69 Laffyn

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Posted 14 December 2006 - 11:14 AM

That's what I'm interested in knowing too, r00kn00b

I think I'll probably do a good cleaning anyways.

:eusa_danc

interesting bandages, are those really waterproof?

#70 Hippie3

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Posted 14 December 2006 - 11:21 AM

Since no unfiltered air can enter
you could inoculate almost anywhere.
just keep needle sterile, no need to flame contstantly
only if it touches unsterile like if you drop it..

#71 Hippie3

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Posted 14 December 2006 - 11:23 AM

i'd just be watchful,
if you see growth is very slow or even stops
that's often a sign of inadequate FAE .

#72 Laffyn

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Posted 15 December 2006 - 02:02 PM

Thanks for the reminder, Hippie...

I'm still trying to decide whether I want to try this 'waterproof' bandaid tek or go with Tyvek.

:greenboun

#73 infinity

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Posted 03 January 2007 - 11:12 AM

Any updates on this tec? A foaf recently bought some bandaid brand water proof bandaids for the test run. All the dollar stores in his town don't carry the water proof, only the normal ones.

#74 Guest_FriendlyNeighbor_*

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Posted 12 January 2007 - 02:51 AM

Hey great stuff. Time for a nice little scavenger hunt tomorrow.

#75 dial8

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Posted 12 January 2007 - 08:50 AM

So no problems with the bandaids melting? The breathable layer does not seal itself shut when heated? Looks like in the first set of pics that you added a piece of breathable tape over the little bandaid. That looks like it has sealed shut.

I tried 3m micropore tape a long time ago and you cannot pc that shit because it will seal itself shut elliminating the breathability.

#76 Hippie3

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Posted 12 January 2007 - 09:25 AM

same problem i had, mine stalled out from lack of FAE .
i believe we got another report in recently that reported the same.
think i'll stick to tyvek and polyfil.




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