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woodlover liquid culture


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#1 waylitjim

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Posted 10 July 2006 - 06:44 PM

This is how I make my wood lover liquid cultures. The process starts with soaking hardwood chips in water over nite. The water absorbs nutrients from the wood chips which makes an excellent broth. The tea is poured thru a coffee filter to strain out any small particles, in this case hickory chips were used. 250 ml of hickory tea was added to the baby bottle and 1 tsp of Karo was added. Here's some of the supplies.

http://mycotopia.net...21&d=1152573545

I used a baby bottle for the LC. The top is snipped off, and the nozzle is stuffed with polyfill.

http://mycotopia.net...23&d=1152573545

The tea wass microwaved for 3 minutes. After it cooled, P. azurescens spores were added.
These pics show the growth 48 hrs later.

http://mycotopia.net...24&d=1152573545

http://mycotopia.net...26&d=1152573545

The LC can be used to create your final fruiting substrate.

http://mycotopia.net...32&d=1152574775

http://mycotopia.net...30&d=1152574775

If all goes well, we'll have another pretty vase of azures.

http://mycotopia.net...28&d=1152574354

http://mycotopia.net...27&d=1152574354

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#2 CannAmerican

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Posted 10 July 2006 - 07:18 PM

Great innovative tek waylit!! I must admit to lurking for some time over your threads, I am just so curious about woodlovers. I've never even eaten any yet! Could you clone an azurescens into that tea? I'm just wondering if it's possible as the stems are so small, I'm not making a request (or am I??) :D

#3 golly

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Posted 10 July 2006 - 07:26 PM

Wow ,,,doesn't get simpler than that...So just 3 mins of nuking is enough..?
May have to give 'er a go....

#4 rockawayrooms

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Posted 10 July 2006 - 07:34 PM

"WOW"what a KILLER of a thread,
you never cease to amaze Jim.:)
Looks like i am going to have to give
this a try real soon:D

#5 shobimono

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Posted 10 July 2006 - 07:44 PM

How do you microwave an lc for 3 mins without it boiling over?
My microwave seems to boil lc way earlier than that. Even stopping it, swirling the liquid, and restarting it will still boil over.

#6 llamabox

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Posted 10 July 2006 - 07:55 PM

Have you tried this with other wood lovers as well?
Say the Ps. caerulipes.....

#7 waylitjim

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Posted 10 July 2006 - 07:57 PM

Could you clone an azurescens into that tea?


You can definitely clone fruits in a LC. This is a nutrient rich solution, so spores and tissue will grow out very fast. Prime sites for tissue extraction are the thick tissue at the base of the stem, and the fleshy tissue inside the cap above the gills. Tear the cap or the stem in half (not cutting, the blade can be a vector for contamination) and a small piece of inner tissue is carefully extracted (with a flame sterilized blade). Make sure you touch and extract nothing but clean tissue from inside the mushroom. If you use the base of a stem, be sure to extract tissue at least a half inch above the base to avoid contaminated tissue that is in contact with the substrate. The excised tissue is then dunked in a 10% peroxide dilution (10ml 3% diluted with 90ml sterile water), and transferred to the woodchip/ karo tea.

Wow ,,,doesn't get simpler than that...So just 3 mins of nuking is enough..? May have to give 'er a go....


I've played around with varying lengths of time. With my microwave on low, the solution is boiling after 1 minute. So the LC gets 2 minutes of boiling. I've had no contamination problems with this technique. I tried 5 minutes, but it seemed like overkill. The solution would usually boil over after 3 minutes.

#8 waylitjim

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Posted 10 July 2006 - 08:05 PM

Have you tried this with other wood lovers as well?
Say the Ps. caerulipes.....


I just finished a LC with Psilocybe subaeruginosa. I'm sure any woodlover would love this brew.

How do you microwave an lc for 3 mins without it boiling over?
My microwave seems to boil lc way earlier than that. Even stopping it, swirling the liquid, and restarting it will still boil over.


I have a slow cook feature on my microwave. It's under the low setting. After 1 minute, it reaches a slow rolling boil. By 3 minutes it's boiling rapidly. I only fill the bottle 3/4 way up so the poly fill doesn't get wet.

#9 Cybin

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Posted 11 July 2006 - 10:41 PM

Hey jim how do you normaly prep your wood chips?

...couldnt really find any info :neutral:

#10 waylitjim

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Posted 12 July 2006 - 12:10 AM

I soak the chips in water for 24 hrs. or untill they're fully hydrated. Rarely do I pasteurize or sterilize the woodchips. I do add bleach to the soak water to kill off any molds or endospores living on the wood.

#11 SharkieJones

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Posted 12 July 2006 - 10:04 AM

Very nice tek Waylit. I'm bookmarking this.

#12 Foster

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Posted 12 July 2006 - 10:40 AM

wow! hey waylitjim, that is about as easy as it gets! great thread and tek! With a little more current experience I just might give ere a go. Truly Sweet! peace pFost

#13 spacecake

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Posted 12 July 2006 - 02:04 PM

Nice tread ,..Waylitjim !

I was just thinking what to do in my summer vacation...!

Now I'm thinking of getting my Subaeruginosa print out of the closet and give your L.C. a try !
Or maybe a Azure..but I don't have a print jet....
...O wait can try a Pan culture clone !
We will see ! (so much to do..so little time...)

#14 waylitjim

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Posted 22 August 2006 - 07:23 PM

Here's some pics to demonstrate another way of using the wood lover liquid culture.

Once the L.C has grown out, prepare some quart jars containing the final fruiting substrate.

This process skips using grains which need to be spawned to the woodchips at a later date. This will save time since it skips a step.

Basically we're injecting 6 ccs of azurescens liquid culture into each quart jars containing the final fruiting substrate.

These are the ingredients:

> 75% Alder Chips (screened in a colander to keep out larger pieces of wood)
> 15% Cactus Soil
> 10% Rye Grass Seed

http://mycotopia.net...33&d=1156291775

> 15% Cactus Soil (composed forest products, peat moss, sand, earthworm castings, bone meal, blood meal, cottonseed meal, and alfalfa meal.)

http://mycotopia.net...36&d=1156291775

> 10% Rye Grass Seed

http://mycotopia.net...34&d=1156291775

These ingredients were mixed together, moistened with water, and pressure cooked for 1 hour.

After they cooled the jars were inoculated with 6ccs of L.C. These jars are now 4 weeks old and are showing some nice healthy growth. The next step will be transferring the jars to a tub for fruiting.

http://mycotopia.net...39&d=1156291775

http://mycotopia.net...37&d=1156291775

http://mycotopia.net...40&d=1156291775

:heart:Waylit

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#15 Lazlo

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Posted 22 August 2006 - 07:39 PM

archive material (lc's and exotics)

I can't believe this one slipped by.

#16 waylitjim

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Posted 22 August 2006 - 11:13 PM

Here's a look inside the jars after removing the lids.

http://mycotopia.net...41&d=1156306166

http://mycotopia.net...43&d=1156306166

http://mycotopia.net...42&d=1156306166

And after dumping the jars into a tub lined with corrugated cardboard.

http://mycotopia.net...46&d=1156306200

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#17 Lazlo

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Posted 23 August 2006 - 11:48 AM

Are you going to fruit the tub outside or inside? With the lid on or off?

#18 waylitjim

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Posted 23 August 2006 - 03:14 PM

I'll fruit the tub outdoors this Fall, with the lid on. (which is clear and lets light in.)

It's the exact same tub I used in my Psilocybe cyanescens grow last season.
http://mycotopia.net...read.php?t=6290

Looking forward to more azures, I find them so enticing. :D

#19 Hippie3

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Posted 23 August 2006 - 06:01 PM

try a few jars invitro just to see
plz
invitro azures would be
phenomenal eh ?

#20 LethalTr1p

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Posted 23 August 2006 - 06:37 PM

where can you find bags of alder chips like that. i cant find them at any greenhouses or nursuries. it says smoking. walmart have them with the grills or something?




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