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Experience with old ass spores?


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11 replies to this topic

#1 petri

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Posted 21 February 2005 - 08:50 PM

I found a spore smeared paper plate folded up in a baggie in the back of my supply cabinet. It is 4 years old. Has anyone used spores that old with any success? Any special reccomendations as to how to breathe life into them? I think this is worth a shot, what say you?

#2 mockeylock

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Posted 21 February 2005 - 11:49 PM

Smeared spores doesn't exactly sound like a clean print. Even if it was clean, it probably isn't now. If you know how to use agar then hit it up and if anything grows, clean it up and see what you got.

Are they old PF spores?

#3 Hippie3

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Posted 22 February 2005 - 08:09 AM

Has anyone used spores that old with any success?

yes.

#4 Hippie3

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Posted 22 February 2005 - 08:10 AM

Any special reccomendations as to how to breathe life into them?

hydrate for a few days before use,
i like to add a very weak dilution of liquid kelp [seaweed] to the water,
seems to help.

#5 the_other_chap

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Posted 22 February 2005 - 06:45 PM

Yep, I've had spores stored in a syringe at room temperature for over a year which germinated with no problem at all, so Im sure your "dry" spores will still be viable.
They may take a day or two longer than "fresh" spores, but if the print isn't contaminated, I'm sure they'll be OK.

#6 petri

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Posted 22 February 2005 - 10:07 PM

Mockey, they are indeed from a strain I got from PF. They are PFC's a few generations after having recieved them. The substrain these came from was off the fucking hook potent, fast and furious invitro pinner. This is worth the attempt. I just want to see some germination, I can clean it up from there. Hippie, where does one look for liquid kelp?

#7 Hippie3

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Posted 22 February 2005 - 10:30 PM

where does one look for liquid kelp?

any decent garden store,
i get mine at a feed store.

#8 Lazlo

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Posted 22 February 2005 - 10:45 PM

Try out Stephen Peele's hot agar tek. It supposedly is a fantastic way to make contaminated spores viable again. It involves growing out a minimal amount of spores on agar in petri dishes. If the print turns out to be contaminated after you see the growth on agar, simply pour another layer of hot agar on top of the growth. The mycelium will crawl through to the top layer leaving behind the contaminate. Rodger even says he vouches for it. I wish i would have known about it. I threw away 100's of prints some years ago due to mold.

#9 nomoreusmc

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Posted 24 February 2005 - 02:00 AM

I've heard 5-10 years with prints. I think it was rodger who was trying to breath life into a 10 year old print!!

#10 altered_states

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Posted 24 February 2005 - 10:55 AM

A year ago I began to isolate a strain from a PF Hawaiian Syringe that was just shy of four years old. It was in the case for a cordless drill the entire time. No special care.

#11 altered_states

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Posted 24 February 2005 - 10:58 AM

BTW, I shot the spores into PF style jars.

#12 anticheffy

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Posted 24 February 2005 - 06:37 PM

did they work?




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