187 replies to this topic

[Hippie3]

maybe folding the tape over on itself
for higher humidity & more available nutes
might be beneficial ?

[fun_maker]

Jar i must try this if contams do not gorw let me know i would like to throw it in some LC myself talk to you soon thanx day is coming see yah then and you have some pink elephants:dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance:EAT THE ELEPHANT:dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance::dance:EAT THE ELEPHANT:dance::dance::dance::dance::dance:.

[the_chosen_one]

if his report of flies carrying spores onto it which grew out is replicated successfully, without the flies of course ;)
the ramifications of being able to germinate spores in open air
under less than sterile conditions
could turn out to be significant, imhfo.

Indeed! If this proves to be more consistent than PF's BRF and Peroxide Tek then it would be quite significant. JaR, I simply must compliment you on your keen sense of observation! :bow: Nicely done!:eusa_clap

[Pedestrian]

Props for the discovery JaR! It could prove most helpful for cloning wild specimens!

Jar i must try this if contams do not grow let me know i would like to throw it in some LC myself

Reminder: just because contams arent growing on the paper, it doesn't mean that the spores arent there. Putting a piece of that colonized paper or a mycelium scraping straight into a LC/grain jar can lead to contamination. Unless the paper was steril to begin with and remains free of contaminate spores, you are probably going to need to take an extra step, like tranfering to a perti or antibiotic media, to ensure you have a truly clean piece of mycelium to grow out.


JaR: you just spray water on a scalpel, scrape some myc, and toss it in the LC? Any other details? How many LCs have you made using this method and what's the success rate?

[Elf Salvation]

hmm

Cool find

[JaR]

JaR: you just spray water on a scalpel, scrape some myc, and toss it in the LC ? Any other details? How many LCs have you made using this method and what's the success rate?

http://mycotopia.net/forums/attachment.php?attachmentid=38779&d=1163080193
I now have 4 quart jars of LC (from fresh tissue) that were transferred this way. Ive did many, many (to many) LCs using this method. At first I cloned everything , the nice clusters, the big boomers, the odd shaped ones,the lighter capped ones, etc, etc, etc.
Ill post pic soon as the ol lady gets up (she kinda gets pissed when i go into the room taking pics while shes asleep):eusa_thin
Ok heres a pic of an LC made using this method LC is around 5?? days old still young
Ive got some bags that should be pinning in a few days made from an LC made this way. The water bottle I use to spray the scapel has some peroxide in it. As for success rate Ive had the occasional WBS jar go green but from flystrip to LC 100%. I transfer from Flystrip to LC in a glovebox.

presumably fresh still moist spores would work better for this
than old desiccated ones.

Ive always used 2-4 days old spore print so your prolly right.
Im just the monkey that used a stick to hit others on the head. You guys turned the stick into a hammer.
:dance:FUN_MAKERs one of the coolest muhfuhs I know. Thanks for the hook-up Bro!!:dance:
Second pic is a colonizing i gallon ziplock bag from WBS using this method
Third pic is a 2 gallon zip lock type bag soon to pin using this method
Both bags

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[dial8]

As soon as I get a damn chance I'm going to start experimenting with this to. I should be able to do something in about 10 days.

I really dig the mouse trap idea. That would be similar to working with a petri dish.

[dial8]

Oh, btw, I have had actively growing mycelium expand from the substrate to some nearby clear tape and it really colonized the tape well. At the time I assumed it was getting its nutrients from the substrate because I've seen myc do the same thing to the sides of plastic tubs.

[Hippie3]

Oh, btw, I have had actively growing mycelium expand from the substrate to some nearby clear tape and it really colonized the tape well. At the time I assumed it was getting its nutrients from the substrate because I've seen myc do the same thing to the sides of plastic tubs.

cool, so that's verification of at least one possibility,
it will grow on ordinary tape

:bow: :eusa_clap

[dial8]

Yeah, and from the way it grew I'm convinced that the tape was nutritious and not just a nice flat surface for the myc to spread out on.

I thought I had posted a pic of it here a long time ago but I can't find it.

[Dutchdude]

This is one cool thread! I agree with other posters, its so great to see new discoveries in this field happen right here in topia.

[Hippie3]

maybe folding the tape over on itself
for higher humidity & more available nutes
might be beneficial ?

just struck by a random thought-
let's say someone took some clear wide tape
sterile from inside the roll
in glovebox/hood
stuck some fresh spores or live tissue, even dried tissue too-
on it
folded it over on itself so no adhesive is left exposed
[trim as necessary]
then placed it to incubate germinate.
i'm wondering if
the mycellia might grow in between the tape layers
to emerge out the sides,
so that one could say
take several such squares of colonized tape-
perhaps even chop/cut it to small bits to increase edges-
kinda like spawn
and mix into a bulk substrate like pasteurized poo
to grow out ?
:eusa_thin
sealed up on itself like that
one could easily disinfect the exterior without harming the mycellia

[Hippie3]

a breathable tape might be good too
but proly isn't necessary is my guess.
medical might be the way, that microporous stuff might well be worth a try
but i'm fairly confident it would also grow out on plain old Scotch* brand clear adhesive tape too, as well as many others.

[Hillbilly]

This is all fascinating, I can see the potential if it works out. JaR's name will be famous!

[Mosach]

This is sweet. As soon as I find some of the tape I'll be trying it.

[buzzmurdoc]

Congrats JaR I believe youve achieved Hero of the Revolution status,this is huge The possibilities are myrad
I was thinking maybe You could dip pot scrubbers into liquid adhesive, let them drain out and use them like cakes ?