JaRs' Fly Strip clone / agar substitute
Posted 08 November 2006 - 08:45 PM
for higher humidity & more available nutes
might be beneficial ?
Posted 08 November 2006 - 10:10 PM
Posted 08 November 2006 - 10:25 PM
if his report of flies carrying spores onto it which grew out is replicated successfully, without the flies of course ;)
the ramifications of being able to germinate spores in open air
under less than sterile conditions
could turn out to be significant, imhfo.
Indeed! If this proves to be more consistent than PF's BRF and Peroxide Tek then it would be quite significant. JaR, I simply must compliment you on your keen sense of observation! :bow: Nicely done!:eusa_clap
Posted 08 November 2006 - 11:42 PM
Jar i must try this if contams do not grow let me know i would like to throw it in some LC myself
Reminder: just because contams arent growing on the paper, it doesn't mean that the spores arent there. Putting a piece of that colonized paper or a mycelium scraping straight into a LC/grain jar can lead to contamination. Unless the paper was steril to begin with and remains free of contaminate spores, you are probably going to need to take an extra step, like tranfering to a perti or antibiotic media, to ensure you have a truly clean piece of mycelium to grow out.
JaR: you just spray water on a scalpel, scrape some myc, and toss it in the LC? Any other details? How many LCs have you made using this method and what's the success rate?
Posted 09 November 2006 - 06:42 AM
JaR: you just spray water on a scalpel, scrape some myc, and toss it in the LC ? Any other details? How many LCs have you made using this method and what's the success rate?
I now have 4 quart jars of LC (from fresh tissue) that were transferred this way. Ive did many, many (to many) LCs using this method. At first I cloned everything , the nice clusters, the big boomers, the odd shaped ones,the lighter capped ones, etc, etc, etc.
Ill post pic soon as the ol lady gets up (she kinda gets pissed when i go into the room taking pics while shes asleep):eusa_thin
Ok heres a pic of an LC made using this method LC is around 5?? days old still young
Ive got some bags that should be pinning in a few days made from an LC made this way. The water bottle I use to spray the scapel has some peroxide in it. As for success rate Ive had the occasional WBS jar go green but from flystrip to LC 100%. I transfer from Flystrip to LC in a glovebox.
Ive always used 2-4 days old spore print so your prolly right.
presumably fresh still moist spores would work better for this
than old desiccated ones.
Im just the monkey that used a stick to hit others on the head. You guys turned the stick into a hammer.
:dance:FUN_MAKERs one of the coolest muhfuhs I know. Thanks for the hook-up Bro!!:dance:
Second pic is a colonizing i gallon ziplock bag from WBS using this method
Third pic is a 2 gallon zip lock type bag soon to pin using this method
Posted 09 November 2006 - 06:47 AM
Posted 09 November 2006 - 09:49 AM
I really dig the mouse trap idea. That would be similar to working with a petri dish.
Posted 09 November 2006 - 09:52 AM
Posted 09 November 2006 - 10:05 AM
Oh, btw, I have had actively growing mycelium expand from the substrate to some nearby clear tape and it really colonized the tape well. At the time I assumed it was getting its nutrients from the substrate because I've seen myc do the same thing to the sides of plastic tubs.
cool, so that's verification of at least one possibility,
it will grow on ordinary tape