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Spore germination in peroxide...?


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#1 golly

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Posted 16 November 2006 - 10:24 AM

This half pint jar appears to show many germinating spores in pure 3% peroxide after two weeks of soaking..

The jar was simply washed and dried, then filled halfway with peroxide..
A fresh whole mushroom was dropped in and just sat there for two weeks..

I was hoping to maybe see some mycelium eventually grow from the shroom itself once the peroxide had nutralized , i never expected to see spores germinate though..
The shroom itself is still well preserved with no sighn of any new growth..

So i'm gonna inject this solution into some spawn jars and find out whats up...Hopefully any contam has been killed by the prolonged contact with the H2o2...

This may go nowhere but then again...

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#2 Hippie3

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Posted 16 November 2006 - 10:30 AM

did you keep that in the dark the 2 weeks it sat ?
peroxide bottles are opaque for a reason...

#3 golly

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Posted 16 November 2006 - 10:59 AM

Jar was in normal room lighting...I wanted the H2o2 to slowly degrade after
nutralizing any potential contaminants...

#4 Hippie3

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Posted 16 November 2006 - 11:04 AM

imagine it actually degraded fairly quickly once placed in contact with the organic compounds of the shroom, it is possible to 'overwhelm' peroxide that way, by using massive amounts of spores/cells- many die but while doing so they also destroy / consume the peroxide, enabling some to survive
and leaving a solution that is now free of peroxide .
test some of it and see if it has any 'fizzle' left...
i've seen this done on peroxidated agar, quite similar-
a thick deposit of spores is streaked on peroxidated agar,
a few survive to germinate
and of course once germinated
the mycellia has the ability itself to neutralize the peroxide safely, as it grows out.

#5 golly

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Posted 16 November 2006 - 11:15 AM

Yeah - bubbles stopped forming on the shroom after about day two..
Could be another way to clean up a print...
One thing i forgot to say , was that after a few days i added a tiny amount of Karo straight from the bottle to encourage clone type growth..

#6 StroFun

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Posted 16 November 2006 - 11:54 AM

so is this an easier way to get germination for a dirty print than agar? I love the experiments on this site!

#7 BigStemz

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Posted 16 November 2006 - 11:58 AM

this is sorta related, but if spores were thrown into the same mixture of brown rice flower and peroxide that is used for the peroxide cloning tek would it growh mycelium?

#8 golly

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Posted 16 November 2006 - 04:25 PM

Tomorrow i can verify under the scope if indeed those are germinating spores..Untill then, i'm just babbling about the possibilities...lol...

#9 chimp

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Posted 16 November 2006 - 04:47 PM

Tomorrow i can verify under the scope if indeed those are germinating spores..Untill then, i'm just babbling about the possibilities...lol...



There is something to be said for babbling.....Some of my best ideas are from babbling......too bad noone around to hear them.:eusa_doh:

#10 viewer6922

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Posted 16 November 2006 - 06:21 PM

I am missing some TEK....I can take 3% hydrogen peroxide .....in a dark jar. place a fresh shroom in it....total darkness for about a week and get some mycillium to innoculate BRF jars?:cry:

#11 golly

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Posted 16 November 2006 - 06:33 PM

There's no tek i know of....so don't bet the farm on it yet...Easy enough to duplicate the method...just don't know the results yet...

#12 golly

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Posted 16 November 2006 - 10:03 PM

just took a sample of solution and scoped it out 60x.....It's def mycelium..

So i'm nock'n up a couple jars to see if any bad guys are riding along...

I'll update in a few days...

#13 safTman

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Posted 17 November 2006 - 10:20 AM

good experiment Golly keep us posted with the results

#14 Hippie3

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Posted 17 November 2006 - 10:39 AM

to be certain that it really IS spores germinating,
not just tissue from the shroom,
one might try suspending a sporulating cap over
a jar of peroxide instead of submersing the cap.
or just shoot a few syringefuls of spores into it,
faster and more direct.

#15 BigStemz

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Posted 17 November 2006 - 10:45 AM

he was using a water/peroxide/ brown rice flower mixture, correct?

#16 real_psily

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Posted 17 November 2006 - 02:20 PM

This half pint jar appears to show many germinating spores in pure 3% peroxide after two weeks of soaking..

I don't think BRF was used at all
He did mention a little Karo around the two week point.
Go Golly this could be another cloning/cleaning tech in the works

#17 Mycorama

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Posted 17 November 2006 - 02:27 PM

to be certain that it really IS spores germinating,
not just tissue from the shroom,


thats what i thought, im sure the peroxide would degrade fairly fast in the light, but having already grown mycelium tissue in the mushroom, i think it would be more likely that the spores didnt germinate, but then again it could be possible to prove it works. keep tryin man! cool stuff.:teeth:

#18 golly

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Posted 17 November 2006 - 05:23 PM

I'm also going to drop a pre-made sporeprint into a peroxide jar ,then add
some karo after 4 days ...
Under the scope there were thousands of tiny threads of mycelium ,some still with little black dots attatched ,which i assume were the original spore
casing..
The jar is still being watched for any eventual growth off the mushroom itself....

#19 Hippie3

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Posted 17 November 2006 - 07:23 PM

i'd test that solution, it may not even be sterile
since peroxide oftens leaves a few survivors behind,
not 100% effective,
more like pasteurization than sterilization .

#20 golly

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Posted 23 November 2006 - 11:19 AM

Here's an update on the progress of that peroxidated mushroom that released it's spores, then germinated in solution...
The growing spores were sucked up in a syringe and injected into three Qt
jars of shakable cake..

After 6 days ,new growth is now visible in several spots in all jars..but appears that only a small fraction of the injected material survived transfer.
No contamination is visible so far...

In the follow up test - Using a spore print in pure peroxide [3%] we are at day five ,,,no germination yet but it's still early ...A tiny amount of Karo was added day two...

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