Spore germination in peroxide...?
Posted 23 November 2006 - 12:11 PM
Posted 25 November 2006 - 08:23 PM
However I do hope I am wrong. I have some rice paper used for making 'picture cakes'. I bet doing spore prints on them and then dropping that into peroxide may make for "instant lc"!!!!!
Either way, congratulations on a fine discovery, Golly. Regardless of the results, you have already shown great promise for this!
Posted 27 November 2006 - 08:15 PM
Speaking of which - these jars are coming along nicely now with no apparent contam...
Posted 27 November 2006 - 08:38 PM
I just created a jar with a maybe a 3rd or a half cup of h2o2 and about a tablespoon of honey. To that I threw in two small Enoki (Flammulina velutipes: http://mycotopia.net...highlight=enoki) pin heads. Each was less than an inch long. Pics to come.
Posted 27 November 2006 - 09:44 PM
I'm still betting that my growth is from spores,,,We should know for sure within 5 days...
Posted 27 November 2006 - 09:54 PM
Posted 27 November 2006 - 11:42 PM
Posted 28 November 2006 - 12:06 AM
Only the peroxide stage is otherwise non sterile...
The whole idea here, is that you could take a wild or contaminated Cap and
toss it into a peroxide jar until mycelium begins growing and is then able to be transfered to spawn media...
Posted 28 November 2006 - 10:02 AM
Since shakable cake was mentioned, this is my second favorite reason for
using it - Maximum germination points from spore nock'n.. now at day 5...
Those are for the mini tray grow...
Posted 30 November 2006 - 08:53 PM
There are some follow up experiments underway with spores,clones and spawn..
Will post anything of note,,,
Posted 01 December 2006 - 02:32 PM
Posted 01 December 2006 - 02:48 PM
better luck with the next batch:thumbup:
dont give up yet.
i've got a piece of stem in straight h2o2 now
it has been in the peroxide for 7 days now
it has new growth and will be inoculating wbs and cakes on monday.
will try to post pic later if interested.
Posted 01 December 2006 - 05:39 PM
I'm not giving up - this is too fascinating and still believe that the potential for a usefull tek is just around the bend...:smokin:
Posted 01 December 2006 - 05:52 PM
Posted 01 December 2006 - 06:12 PM
Just looked at the jars and they seem to be OK, i dunno...Yesterday it looked like a powdery mold in spots ,i never saw green but thought it was the prelude.
What a drama queen eh...lol..Anyhoo I'm gonna spawn em to get the real story...Here's a pic..They smell shroomy...