Spore germination in peroxide...?
Posted 01 December 2006 - 09:01 PM
did you put some of the myc on agar by any chance, just in case?
your experiment led me to wonder. if you poured a strong h2o2 agar plate,
strong enough to 'kill all known germs dead' as it were,
might increase your success rates in a glove box.
degrade the h2o2 with strong light source before introducing the myc.
if you could fully degrade the h2o2 might it work with spores.
but then, i was thinking maybe a couple of the cats buiscuits ala hippies3s,'everwhelm' paroxide, organic compounds idea. as an additional food source to the karo. mainly because i'm outta malt. dont laugh,i found myself studying a pakage of spirulina in a health food shop recenly apropos myc food...if i'd had a spare £12 ...even tried to blag a free sample from a manufacturer lol.
really 'dig' this experimenters corner.
just read. golly..."Using a spore print in pure peroxide [3%] we are at day five"
this would be the degraded peroxide wouldn' it?
Posted 01 December 2006 - 09:22 PM
Posted 01 December 2006 - 09:57 PM
Posted 02 December 2006 - 09:33 AM
Posted 02 December 2006 - 12:52 PM
remember it is just straight 3% h2o2 and inside of a stem
what do yall think?
Posted 03 December 2006 - 09:42 AM
Seriously it's looking great to me... Is there no air exchange set up for this jar? I'd guess the oxygen introduced with the peroxide would be consumed at some point and it might drown, at least that's what I'd be watching out for.
Nice lighting for the pics, BTW...
Posted 03 December 2006 - 10:59 AM
One day after spawning to the minitrays - the myc is kickin and all looks normal at the moment....Proly take pic tmrw....
Posted 03 December 2006 - 12:18 PM
after looking back at the calender its been more like 12 days since i started this project. the jar was wiped with alcohol and then peroxide poured in. the stem was torn in half, and a new unused needle was used to extract the inside part of the stem. the stem was picked about 12 hours before and left on a paper towel until it was needed. this was done in open air, just a freshly cleaned bathroom with no air movement. so it may not be clean? I already had my GB full and this was more of an after thought just for kicks so we will see how this turns out.
BTW in the pics i had not shaken the jar yet, will shake before I draw up the syringe. the jar has a couple of self healing injection ports but no way of air exchange. like TVC said its probably used up all the air but it appears to have enough growth to be drawn up for inoculation.
Posted 04 December 2006 - 09:25 AM
Here are the trays at 48hrs from spawning ...They're lookin good with some developing rhizo's ..will be casing em later today...no sighn of mold at the moment..
I think what was happening - the cake spawn was a bit on the dry side and the myc appeared mold-like on the drier spots...
Posted 04 December 2006 - 09:38 AM
I suppose you still have no growth in your straight spore jar?
Posted 05 December 2006 - 12:45 PM
the jar has a couple of self healing injection ports but no way of air exchange.
you can give air exchange manually by using an airport in each port
to force fresh filtered air in , letting old stake out.
Posted 05 December 2006 - 01:42 PM
Possibly the higher ratio of peroxide to organic matter may be delaying growth or the added karo was oxidized...I'm gonna add a bit more -see if that helps...
If the original growth from that mushjar which i just cased in 4 minitrays fruit, then they should all look pretty uniform ,if indeed that growth came from the shroom and not the spores...dont cha think...?
Posted 07 December 2006 - 10:38 AM
Another peroxide test , this time with dried frozen Cubes placed in a halfpint 9 days ago, are showing similar formation of myc - all growing in small fragments on the jar bottom and nothing on the main material thats floating...I swirled a bit to photo...
There are 2 jars in this test and both are showing growth ..
If these can sucssessfully be transfered and grown out - then i think i'll
write a Tec on the third try...Meanwhile, try it out peeps - it's very simple..
Posted 07 December 2006 - 11:25 AM
Posted 07 December 2006 - 07:38 PM
I'm thinking now, that Bobcat's idea maybe correct - in that tiny fragments of the shroom are broken off through the oxidizing proccess and growing out on the jar bottom...The spore only jar is now at day 20 with no sighn of life...Hey maybe peroxide really does kill spores :lol: Captain obvious sighning off...
Posted 12 December 2006 - 05:14 PM
Also do you guys think the peroxide is enough to sterilize a jar? If so that's amazing as far as cloning teks go.
For example, would a airtight jar with peroxide in it be sterile. Or are there spores/bacteria that peroxide will not kill?
Posted 12 December 2006 - 07:05 PM
If the duration of contact with peroxide inside the jar is long enough - i suspect that any spore or bacteria will be oxidized..I am testing that theory
in various ways..will post as things develop...May have to change thread title as this is going into different territory...
Posted 12 December 2006 - 07:06 PM
I have been seeing a lot green in mine but I have some more things I am going to try- including using a stronger peroxide. I have some 35%, but its an older bottle thats weakened considerably. I have one jar that seems to be doing well.
Those are good questions Primate, and through more experimentation we will find out I guess. I think that with fresh peroxide at full 3% or more strength, without anything else in it, the jar would be sterile- or close to it. But the stuff breaks down so quick.... I think the trick here is going to be figuring out the ratios.
(# ounces, % peroxide) plus (# grams of sugar) plus (# grams of fresh mushroom) equals SUCCESS!
Posted 12 December 2006 - 07:08 PM
Does anyone know what happens to sugar when its oxidized? What is the final product and is it useable to mycelium?