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Spore germination in peroxide...?


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#101 Bobcat

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Posted 03 February 2007 - 12:33 PM

If you find it, please be careful with it. Def not the same as 3%. It can seriously harm you.

#102 safTman

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Posted 03 February 2007 - 03:05 PM

Sorry for the delay but with work I have been busy. Here are two trays from the clone jar in the eariler post that i expanded into more WBS then spawned to Hpoo and coir. The clone in the h2o2 (the finger) LOL was from a nice cluster and as you may can tell from the pics most all were clusters. The last pic is a small spice extraction project of a FOAF. enjoy

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#103 golly

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Posted 03 February 2007 - 03:22 PM

Well that turned out really nice Saft,,,Using the inner stem tissue really adds to the success potential....Sweeet....:thumbup:

#104 pilot22

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Posted 03 February 2007 - 04:32 PM

.
Very impressive photos, SafTman... maybe you could tell us
why your H2O2 methods succeeded so well, compared to the
others mentioned?


"If you find it, please be careful with it. Def not the same as
3%. It can seriously harm you."


Thanks, Golly, your concern is much appreciated. But I was a
chemistry major in college... specialized in high explosives...
peroxides were a known caution for us...


pilot22
.

#105 safTman

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Posted 03 February 2007 - 06:06 PM

.
Very impressive photos, SafTman... maybe you could tell us
why your H2O2 methods succeeded so well, compared to the
others mentioned?
.


No idea why. Other than i used no karo just straight h2o2 if you look at the photo of the inner stem in the peroxide you can tell it was ready to GO. Like i said earlier, not very sterile conditions while i transfered the inner tissue. May have just got lucky this go around, I will need to duplicate this experiment again to be sure. Thanks to Golly for bringing this idea to my attention.:bow:

#106 pilot22

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Posted 07 February 2007 - 07:40 PM

.

Oh, okay... so you used straight H2O2... which is of course that 100%
stuff they used in rocket fuel. Wow!


Just kidding... but maybe it would help us all if we knew what you meant
by "straight." Is that 3% (antiseptic), or 6% (hair bleach), or
something even higher?


The odds that you "just got lucky" are, in my opinion, astronomical, with
the conditions being, as you said, "not very sterile." You did something right,
and I think you may be onto something here,
something very useful to know.


Please do keep us informed of any progress. I'm sure there are many
of us who will be watching with great interest. And even if the whole
thing bombs out, we can still learn from it.


pilot22

.

#107 golly

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Posted 07 February 2007 - 08:46 PM

Pretty sure the only difference between Saft's grow and mine was, he used a cleaner tissue sample...Mine was an entire mushroom dropped into the peroxide ,,Even without a nutrient solution there is apparently enough of a food resevoir in the tissue to initiate new growth...

#108 Hippie3

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Posted 07 February 2007 - 08:56 PM

..."If you find it, please be careful with it. Def not the same as
3%. It can seriously harm you."
Thanks, Golly, your concern is much appreciated. But I was a
chemistry major in college... specialized in high explosives...
peroxides were a known caution for us...
pilot22
.

you might not have noticed this yet
but you aren't the only one here...

.
Oh, okay... so you used straight H2O2... which is of course that 100%
stuff they used in rocket fuel. Wow! ...pilot22
.

you're pretty new here to be being so damn sarcastic,
pretty much safe to assume he meant 3% as that's
about all one can find in the USA and if he had
something special then he'd mention that.

#109 pilot22

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Posted 08 February 2007 - 10:38 PM

"Oh, okay... so you used straight H2O2... which is of course that 100%
stuff they used in rocket fuel. Wow! ...pilot22"


"you're pretty new here to be being so damn sarcastic,
pretty much safe to assume he meant 3% as that's
about all one can find in the USA and if he had
something special then he'd mention that."


Sarcastic? Nope, that's called "kidding around." (In fact, if
you'll read the next paragraph of my post, I even say I was
kidding.)


And as for assuming he meant a 3% concentration, in science we
learn not to assume anything. I merely was asking for clarification,
since he hadn't stated it -- and in the U.S., it is pretty easy to find
3%, 6%, even 35% (found at some hardware stores).


Sorry if you had a bad day at the office and I rubbed you the wrong
way, but that's life in the big city. SafTman and Golly are making what
may be some very important contributions here to the art of avoiding
contamination -- and I want to learn from them. If attacking a Newbie
just because you think he's been sarcastic is your idea of being a Site
Administrator, then perhaps you should ban me.


Either that or perhaps try a little harder to . . . "lighten up"?


pilot22

#110 safTman

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Posted 09 February 2007 - 10:25 AM

well here is a few pic of the stem growth
remember it is just straight 3% h2o2 and inside of a stem
what do yall think?



Pilot, if you would take time and research the rest of this thread
you would have known that i have already stated 3%. Check out the
first page of this thread.

#111 pilot22

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Posted 09 February 2007 - 01:13 PM

'
Y'know, I like you and admire the work you guys are doing so much
that I think I'll just take a hit on this rather than point out that you
last mentioned your peroxide strength a week ago -- and I should
have "assumed" you would never have tried anything stronger after
that.

Maybe Hippie3 was right, maybe I was stupid to think you may have
changed the formulation -- but after all, you were somehow
succeeding now, and I was determined to get to the bottom of it. :loveeyes:

At any rate, I will try harder not to tromp on anyone's toes here again,
now realizing they may be far more tender than any I have
encountered in similar forums . . .

After all, I believe we are all after the same goal: a growing method
requiring far less time, care, and effort than heretofore. You gents are
pioneers in my mind, and may someday deserve to have your names up
in lights along with those of Wasson, Schultes, McKenna, and
MacPherson (a few personal favorites of mine.)

My best to your and your innovative research!

pilot22

'

#112 StroFun

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Posted 12 February 2007 - 10:03 AM

mmm mmm mmm people these days

so i believe the conclusion was that agar and sterile technique are still best?

#113 shadowechos

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Posted 13 February 2007 - 05:02 PM

mmm mmm mmm people these days

so i believe the conclusion was that agar and sterile technique are still best?


Yup-overall, generally speaking...

35% HP is available on-line in "food grade" and is used by some health minded folks. Non food grade MAY have some undesirable additives.

And is also availble for use in swimming pools/hot tubs.

35% will burn skin and turn it white quickly and easily, and should be kept in the freezer, and may require hazardous shipping in large quantity. Cautions and info. can be found on-line.

#114 TVCasualty

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Posted 14 February 2007 - 07:52 AM

I can get 35% peroxide at my local health food stores, right off the shelf. Most all health food stores use the same set of catalogs to order their inventory from, so just ask if they have it, and if not they can order it for you. FYI: it's often used by 'disaster preparedness' afficionadoes to keep stored water fresh and potable for long periods of time, in case you want to have an 'excuse' to buy it.


Pilot22: the toes around here are not particularly sensitive, it's just that when a group of people are standing around talking, and they have been doing so for a fair bit of time, they tend to look askance at someone sauntering up unannounced and taking a somewhat mocking tone (made all better by saying "just kidding" of course... just kidding.) then following it with passive-aggressive ambiguity: "I think I'll just take a hit on this rather than point out that you last mentioned your peroxide strength a week ago" (that was one of the passive-aggressive parts, the ambiguous part was the whole first paragraph). I'm really clever as well, and many members around here would agree that when I put my cleverness to robust use, I tend to sound like an ass, and am received as such (should I be surprised?). So much for being clever.

And what college allows chemistry majors (a phrasing that implies the undergraduate level) to 'specialize' in high explosives (I want to make sure I don't live near one!)

#115 pilot22

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Posted 14 February 2007 - 04:07 PM

'



First of all, I'd like to thank you and Shadowechoes for confirming my
statement that 35% H2O2 is not all that hard to obtain, which seems
to be what started all of this:


"pretty much safe to assume he meant 3% as that's
about all one can find in the USA and if he had
something special then he'd mention that."



I agree with your statement, "I'm really clever as well, and many
members around here would agree that when I put my cleverness to
robust use, I tend to sound like an ass, and am received as such
(should I be surprised?). So much for being clever."



Yep. I agree. You are both clever and bright. It's nice to know there
are two of us. And I'm sorry if I sounded like an ass. But the way
you seem to be more interested in squelching a newcomer -- who,
admittedly, may have not been as delicate in his phrasing as
you'd have preferred -- than in learning more about a technique
that could possibly make mushroom growing a simple matter for those
who would like to grow spiritually and explore their own consciousness,
makes me wonder what your real agenda is.



Personally, rather spend time with this bickering over phraseology, I
feel that we may just be able to do some good in the world by pursuing
these techniques. And as I have said, I do admire the sincerity of
those taking part in it... especially those who see it as an important
endowment for mankind, rather than simply as a way to make some
fast money.



"And what college allows chemistry majors (a phrasing that implies the
undergraduate level) to 'specialize' in high explosives (I want to make
sure I don't live near one!)"


Very well, here is what I said:

"... I was a chemistry major in college... specialized in high explosives...
peroxides were a known caution for us..."


The ellipsis, as generally used, indicates something was left out. There
was no connection between my college chemistry courses and any
later experience in explosives -- and if I'd known you had been planning
on moving close to only a Low Detonation Type College, I certainly
would have made this clearer. :eusa_shif


Now I don't know about the rest of you, but I am still very much more
interested in learning what Saftman, Golly, and the others have
discovered . . . than in continuing with this . . .



With affection,

pilot22

'

#116 TVCasualty

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Posted 17 February 2007 - 01:18 AM

Now I don't know about the rest of you, but I am still very much more interested in learning what Saftman, Golly, and the others have
discovered . . . than in continuing with this . . .


If that were true it would have been the entirety your post.

My real agenda? Tidy information flow. Stomping out pomposity.

I had begun another reply, but then I noticed the words "former member" under your avatar, so I offer this instead. Apparently my character prescience is again vindicated, though I know not the mechanism of the 'former' status. I am certain that you will log back on here and read this, which is kind of funny. An ability to write in a patronizing manner is not the same as good writing...masking obfuscatory obtuseness with vocabulary...eating bratwurst too early in the day.

(Ellipsisises! I wonder what was left out?!? There is no connection between your writing and bratwurst, just thought I'd link them without capitalizing the first word in each pseudo-sentence to imply a connection inadvertently and then backpedal from it's ambiguity later by citing a not-quite-technically correct use of an element of punctuation)

My apologies to everyone else for the :offtopic: nonsense. It is now over.

Now where were we? Oh yes, you can order your 35% peroxide from your local health food store.




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