49 replies to this topic

[kikashi]

First Z casing attempt. 5 BRF cakes cased 50/50 verm/peat. 6th cake left to fruit has produced about 60 g so far over 3 flushes. This? Maybe 30 when these grow out? Pathetic. Give it till the 8 jars currently incubating are coated. Then it's trash time if it hasn't produced. Need the space to do a little selective EQ breeding for altitude..

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[kikashi]

Two of the babies below came out at 28g. Looks like a little more to come.


Question anyone. Can I use the grain from a beer supply store? Typically those are malted grains. Or do I need to find raw fresh grain. Like from farm suppliers.

[cutty]

Im really not sure man but I buy mine from a farm supply store or a bulk health food store.

[kikashi]

thx cutty. I'll look around. It's a question of pre-malted vs whatever malting happens to happen during the pasteurization process.

One more question. Tyvek bags - can get em by the roll. 100/roll. 12 rolls per case. I don't anticipate needing 1200 bags however. Anybody know a link for just a box? The ones I'm looking at are medical grade/ $15 a roll or box. I believe they're 10" x 15".

[kikashi]

Six weeks to coat, 6 days from dunk. EQ spore syringe.

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[kikashi]

nice bunch of pins but seems to be stalled here. EQ cake from spore syringe.

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[kikashi]

Jelly jar update 10 days from innoc.: 8 of 8 colonizing, 2 clear front runners, 1 real laggard. Hope the photo session didn't get them contammed. None so far.

Both Bonne Maman jars are coating, one is one of my 2 leaders. If the jar on the lower R (facing them), is done in 11 days it'll be a record for me by 1 day.

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[Soliver1]

Malted grains? Hmmm .... probably best to stick to what we know works.
It would probably work, but the additional carbs that have been converted into
sugars by sprouting / toasting my provide additional and unneccesary vectors for
unwanted growth / contamination.

:)

soliver

[kikashi]

thx, have found the requisite grain in the interim. I'm finding a little malt extract works well with PFT jars. Just askin.

[kikashi]

this was nice to wake up to!

oh well pic later maybe

[kikashi]

ok uploading now - Thankyou Zen, et al. This is EQ, 1st flush, 38g fresh.

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[depayne66]

I can attest that kikashi's malt additive works - I've seen it side by side with unmalted jars, and there's no comparison. I personally believe it'd be contam'd all to hell, though, if he didn't dunk into - and aero-hydrate routinely with - H2O2. And doesn't the CO2 given off at the early stages boost pin development - or do I have it backwards...

[kikashi]

Actually, I use a 20% peroxide/water solution from dunking on. It's nowhere near those jars however. Peroxide would wipe out the spores I'm trying to get to colonize.

[depayne66]

... Peroxide would wipe out the spores I'm trying to get to colonize.

Meant "cakes", of course...
still think peroxide is the ideal contam foil, tho'

[kikashi]

one of 2 frontrunners. coated in 13 days, left in the incubator an extra week. Moved them into a fruiting chamber today. I want to see if the drop in temp and humidity will get rid of the wet in there. Also put together a quick and dirty glove box. It has low rate positive pressure sterile air. The gadget is a combo electrostatic & ultraviolet air cleaner. The insert in the vent is a radio shack computer fan. Should be able to share some prints soon.

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[Lazlo]

Meant "cakes", of course...
still think peroxide is the ideal contam foil, tho'

Peroxide is the ideal work slower downer IMO. LOL! Actually; not an opinion, a fact. Forget h202 for mycelical growth. A mild bleach solution is superior to h202. (1:200) parts!!!!!!!!!!!!!!!!!!

[kikashi]

Just to make this real clear. I currently have 8 PFT+ jars in various stages of coating/ pre-pinning. No peroxide or bleach has been used in the process. Just PC'd the tinfoil covered jars, ( Tyvek from here out), and innoculated under very clean conditions, ( didn't have the glove box yet). So far no contams. Had 1 contam. in the last group of 12. That's 1 contam per 20 jars innoculated. I can live with that but, it'll probably improve with the glove box.

The cap is a Z, it's printing at the moment. Stem was 12g fresh.

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[kikashi]

EQ's I want to clone are starting up. Mycelium or shroom parts? The one time I tried myc. it colonized realy fast. But it was a really strange shroom. The larger cap is printing now.

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[kikashi]

sorry, no prints for now. I made the mistake of using Tyvek. Unfortuneately it's too slippery a surface. they came out a mess of loose spores as soon as I moved them.

[candykid420]

on the last set of pictures, what is the strain in the left picture?