1.) put a 'faht jar' in an oven bag and zip tie leaving a good portion of the rest of the bag for the 'quarantine' section. I forgot to put it in the diagram, but you need some kind of air escape in the part of the bag that the jar is in or the bag will pop in the pc. i use 5/8 in. tubing stuffed with polyfil thats zip tied to the bag. I actually put one in each corner of the bag just in case. now pc this at 15 psi for 35 min.
2.) once cooled.. take out of pc and get your cloning tissue ready. Take an inner piece of tissue and sterilize in H2O2 3%with a sterile tweezers until it stops 'fizzing'. now take the tissue and put it in the top part of the oven bag right on top of the zip tie. Pour a generous amount of H2O2 3% over the tissue in the top of the bag. then squeeze out any extra air and zip tie that off. massage the tissue around in the 'quarantine' section for a minute or two.
3.) Now unscrew the jar lid (i'm using lids from this thread...Lids That Have Stood the Test of Time. -Mycotopia Web Forums
using your hands thru the bag.. DO NOT OPEN THE BAG AT ANY TIME. the bag is easy to manuever from the outside.
4.) now CAREFULLY (so you don't cut the oven bag) cut the first zip tie so that the H2O2 and tissue 'flow' into the main part of the bag that the jar is in.
5.) while the bag is still closed, use your hands to maneuver the tissue into the jar (it's actually quite easy). Now screw the lid back on and tighten. again, while it's still in the bag.
6.) NOW you can remove the jar... shake the hell out it and it will be colonized in 4 days. then i just break it up, inject with water, shake it around, and suck up the myc. water to be used as innoc. :) heres the diagram....
Fahts self-healing lids and bag patches - Mycotopia Web Forums
Edited by coorsmikey, 09 March 2017 - 11:49 AM.