122 replies to this topic

[Xerces]

Ok I'm planning to recieve my print on friday or monday. So I have my itinerary all set:

Necessary Items:
@ Spore Print (Brazil Strain)
12/24 Glass Jars with lids
Brown Rice Flour
Vermiculite
Perlite
1 Tinfoil
1 Pressure cooker
3 Syringes
2 Large Rubbermaid transparent tapperware whatever
1 Large card box + 1 transparent something for Glove-Box
1 Pair of Gloves
2 Mist Sprayers
1 Lysoform Dessinfectant
1 Lighter
1 Thermometer
1 Hammer + Nail
1 Alcohol


Now here is the schedule:
Xerces 12 Easy Steps Tek

Wednesday
1. Preparation of the Substrate and Jars
-> Punch 2 holes with nail and hammer.
-> Prepare 2 Parts Vermiculite, 1 Part BRF, 1 Part Bottled Water, leaving space for air.
-> Cover lightly with lid and tinfoil.

Thursday
2. Sterilization of the Jars
-> Pressure Cook the Jars

Friday or Monday - Recieving the print
3. Dessinfection of the work area
-> Alcohol/Water Spray

4. Syringe Creation from Print
-> Scraping from print with flamed knife into jar, then adding water with syringe and sucking it all back in.

5. Inoculation
-> Inject Spores into Jars through holes, quickly replacing the tinfoil.

6. Incubation (Darkness, aprox 28*C)
-> Store the jars in the tapperware, into a black bag, and into a closet.

Saturday / Thursday / following days
7. Shake Jars, break up mycelium, inoculate extra jars with the colonized substrate.

*> Perhaps isolate ropey ryzomorphic strains.
*> Perhaps storage of mycelium single strains.

8. Casing and Terrarium (Lightness 12hs, aprox 23*C)
-> Perlite + Water Casing
-> Distribute Mycelium on Cased Trays into Big Tapperware
-> Store in light spot with small holes for air.

9. Fruiting
-> Watch and Document Growth.

10. Harvesting / Live tissue sampling / Spore Printing

11. Dunking and preparing for next flush

12. Eating / Drying / Storing

I'll post pictures of the process, and ask for advice!



13. Tripping

Edited by Zen_, 10 August 2014 - 02:44 PM.
redacted

[Mortandello]

good luck, neighbor!

[dial8]

Good luck and keep us posted! Pics are always welcome!

[Hippie3]

nicely written post
:bow:
just one quibble -
i'd use a different color next time,
your orange is very close to our yellow links,
blue or green, etc. would make better distinction .

[spacecake]

Don't forget your verm. barrier on top (wednesday) !

And leave out point 7 ,..don't shake your (BRF)jars !
Do you know how to isolate ?..if your new to this,.. then keep it simple the first time !

Good luck !

[Xerces]

Lol ! Ok, I'll try to keep it simple! I thought I could break up BRF cakes just like rye seed cakes. How is the casing done? Can i break up the mycelium?

Today I went to see the rubbermaid tapperware and saw some big boxes and like 3 drawers made of rubbermaid, that was interesting.



I also was looking for Jars, but just found plastic containers. That cant be pressure cook I think.. melts?

There were also some.. big jars, like 1 liter glass jars with red lids.
Any input on what should I choose? Price is the key concern.

edit: the verm layer you are reffering is in the jars and its a layer of DRY verm, right?

[Xerces]

Isolating is by removing live tissue, or rhizomorphic ropey mycelium, right?
I watched this very usefull video, I dont remember who in the forum gave me the link..
Mushroom Growing Made Easy

[rustyshackleford]

moi!
Yes it is okay to break up the myc.
BRF cakes do not break up as easily as rye. you will have to put the cake into a plastic baggie and use your hands to actually tear it apart. you can use a fork too.
don't be too afraid of getting it contam'd, as the cake, if you are at the stage of casing it, will hopefully be fully colonized by then.

as far as jars go, if you are making brf cakes, you will need to get jars with no shoulders and they are more beneficial if they were also tapered.

just search for kerr, ball or bernardin (canada) jars. they make canning jars. the correct jars to go into the pressure cooker.

and i believe he is referring to the dry layer of verm that will be used as a barrier.

[Xerces]

So this is what i bought so far:

The "terrarium" tapperware;
2 boxes of AR$32 Pesos total. (u$s 10.60)
2 Sprayer bottles AR$ 6 Pesos total (u$s 2)
Alcohol AR$2 Pesos (u$s 0.60)
Pressure Cooker Borrowed from my girlfriend -priceless-
Jars: 2 Nutella Jars, and 1 Noel Mermelade Jar.

Cellphone and keys only for size reference.

By the way, whats the easiest way to create the holes in that hard plastic terrarium? Burning maybe? How big should the holes be and located where?

I still have to get some more jars, and the brown rice flour, the vermiculite, the perlite, syringes, gloves, a cardbox for glovebox and lysoform. Although I think maybe a water/alcohol96% 90/10 mix in the sprayer might work as well.

I'm also waiting for the spores, hopefully they'll get here by friday or monday at least. I feel like a child before his birthday.

Thanks
Lukas Lain

Attached Thumbnails

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[Xerces]

Wait a minute.. can one use Tinfoil Covered Glasses instead of jars?

[Xerces]

Ok so this I've got almost everything except the cardbox for the glovebox I'll buy later, and perhaps lysoform. A termometer would be nice too, but I'm not sure how much it costs.

Brown Rice Flour x 1 KG = AR$ 2.60 / U$S 0.86
Vermiculite x 3.5 DM3 = AR$ 6.00 / U$S 2.00
Perlite x 5 DM3 = AR$ 6.00 / U$S 2.00
Syringes x 2 = AR$ 1.80 / U$S 0.60
Gloves x 2 pairs = AR$ 0.80 / U$S 0.26
Tinfoil x 5 M = AR$ 2.99 / U$S 0.99
*Cardbox for Glovebox = AR$ 4.00 / U$S 1.30
Cellophane for Glovebox = AR$ 0.90 / U$S 0.30
Plastic Containers for Fruiting x 2 = AR$ 32.00 / U$S 10.60
Sprayer x 2 = AR$ 6.00 / U$S 2.00
Pressure Cooker = Borrowed
*Lysoform = AR$ 4.00 / U$S 1.30
*Spores (Brazil Strain) = Gift

Total expended for all materials = AR$ 67.10 / U$S 22.36

{ * = Don't have yet }


http://mycotopia.net...=1&d=1165593919


p.d.: what do you think about using glass cups well covered with tinfoil instead of glass jars with lids?

[San Mescalito]

I use glass cups, and they work grate, just put thin foil on top tape it to the glass, then put another thin foil as the cover with a Rubberbands, then when you inoculate you take this one out, you inoculate going through the first layer of thin foil and then you cover it up with the second one again and put the rubberband, the only down side is that you don’t have air exchange, but it works any way.

[Xerces]

I've prepared the substrate using

1/4 BRF
1/4 Water
1/2 Vermiculite

Leaving about an inch of dry verm layer on top.

http://mycotopia.net...=1&d=1165610285
http://mycotopia.net...=1&d=1165610285


Used 3 glass cups, 2 nutella jars and 1 mermelade jar :P
Covered with tinfoil and stored in plastic container until tonight when I sterilize them.
http://mycotopia.net...=1&d=1165610285
http://mycotopia.net...=1&d=1165610285


The only problem is that only 5 jars fit in the PC.

http://mycotopia.net...=1&d=1165610285

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[spacecake]

Looking good !:thumbup:

[Hippie3]

great pic!

http://mycotopia.net...=1&d=1165593919

[loochypooch]

Saturday / Thursday / following days
7. Shake Jars, break up mycelium, inoculate extra jars with the colonized substrate.

You plan on shaking a BRF/Verm (pf?) jar? Is that normal, I thought that was done with grain only.

[Xerces]

moi!
Yes it is okay to break up the myc.
BRF cakes do not break up as easily as rye. you will have to put the cake into a plastic baggie and use your hands to actually tear it apart. you can use a fork too.
don't be too afraid of getting it contam'd, as the cake, if you are at the stage of casing it, will hopefully be fully colonized by then.

as far as jars go, if you are making brf cakes, you will need to get jars with no shoulders and they are more beneficial if they were also tapered.

just search for kerr, ball or bernardin (canada) jars. they make canning jars. the correct jars to go into the pressure cooker.

and i believe he is referring to the dry layer of verm that will be used as a barrier.

I guess :)

[Xerces]

Ok I just sterilized the jars with the PC, just one thing... I smelled a little..... BURNT RUBBER... Now wait a minute... the glass cups had rubber bands around the foil covers!

Well, guess I'll find out what happened when it cools down..

[San Mescalito]

Hello
I always use rubber bands around my second tinfoil cover, I sterilize in pressure cooker for 2hrs, they had never melt, burn or got glued to the tinfoil.

But in your pictures we don’t see de rubberbands?!

[Xerces]

The rubber bands cant be seen because there is another foil layer on top of them.
Luckily they didn't melt or burnt :)
Everthing sterilized, now I just have to wait for the print :)