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Salt=Contamination Inhibitor?


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#21 Bobcat

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Posted 07 January 2007 - 07:36 PM

This is a great experiment and its looking good. :bow:

One potential issue down the line could be plate evaporation/ salt accumulation. Nothing to worry about now, of course, but may need to attend to that later. Any ideas for future trials? See how well the green does on it? Lots of possibilities here.

Nice idea! Hope it works! I also hope your blewitt goes purple!

#22 Lazlo

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Posted 07 January 2007 - 07:39 PM

Will the Blewit mycelium turn purple with age Bobcat?

#23 bandog

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Posted 07 January 2007 - 08:05 PM

I tried a search here with no luck ... looking for Golly's spore germination with peroxide you refer to ... can you point me to it?

thanks! :)

#24 Bobcat

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Posted 07 January 2007 - 09:10 PM

I don't know if it is with age, size or substrate, but my understanding is that it can develop hues of purples and pinks in time. I also don't know if every strain does this as I have seen fruits that are only kind-of-bluish white/tan and others that are really pink/purple. So maybe strain dependent?

Here's something I do know about them: they are one of the 'predatory fungi' that will seek out and destroy bacteria colonies. Possibly as a food source. Neat, eh? Maybe they need to feed on the living to go purple!

Here is a really neat read on the subject that actually explains quite a bit about mushroom life:

http://www.uoguelph....C2003/feb03.htm

Sorry to go off topic. Back to your experiment!

#25 Lazlo

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Posted 07 January 2007 - 09:19 PM

You're not off topic at all. I don't even know for a fact that Blewit is the mycelium here. It could be a contaminate, as can be the King. But I highly doubt it. As I was very cautious and clean with this one.

It would make sense that the Blewit mycelium would turn purple with age or a drying process, as it stains purple once injured or gets old.

#26 yahutli

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Posted 07 January 2007 - 09:19 PM

I had no idea...

That box is way cool. Got any specs?

You guys rock!!!

So where can I read more about this dry tissue inoculation?

#27 yahutli

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Posted 07 January 2007 - 09:42 PM

Anyone tried grapefruit seed extract as an anti-contam agent?

#28 Lazlo

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Posted 07 January 2007 - 10:26 PM

Nope, but considered lemon juice. Same difference I guess.

#29 Hippie3

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Posted 07 January 2007 - 10:39 PM

... where can I read more about this dry tissue inoculation?


under cloning , i believe, in the archives and vaults sections here

#30 Freaky

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Posted 07 January 2007 - 10:59 PM

Lazlo,
You know I totally love all your great experiments, and this one I really enjoyed reading! Great pics too!

I was always wondering how tea tree oil or garlic may work with agar. I guess I've always just stuck with h2o2 simply because it always worked as planned and the hot agar pour by Peele.
Your work with this is inspiring and I'm enjoying watching it unfold!

#31 Bobcat

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Posted 07 January 2007 - 11:18 PM

I'd thought of garlic too. Capsaicin (chili pepper powder) as well. Got so far as to order the chili pepper and then forgot about it, lol, until just this second!

#32 yahutli

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Posted 08 January 2007 - 06:02 PM

Check out this link about GSE
www.pureliquidgold.com

some interesting properties...

#33 yahutli

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Posted 08 January 2007 - 06:03 PM

Thanks hip:teeth:

#34 Lazlo

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Posted 08 January 2007 - 06:11 PM

I thought about tea tree oil too and forgot. That could be promising. So far so good with this project. I think I may go ahead and do the Cauliflower too.

The only problem with SLP's hot agar pour as it does squat against molds. Bacteria on the other hand it works great.

#35 Freaky

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Posted 08 January 2007 - 09:12 PM

The only problem with SLP's hot agar pour as it does squat against molds. Bacteria on the other hand it works great.



I agree Lazlo, but it does work well if bacteria is a problem.

I love the work you all are doing to test this. I might have to power up the hood and mess with some agar dishes :D

#36 Lazlo

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Posted 10 January 2007 - 02:36 PM

Looking good.


http://mycotopia.net...=1&d=1168457763

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#37 dial8

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Posted 10 January 2007 - 02:45 PM

Yeah looks real nice, laz. If I recall correctly you used some pretty dirty prints, right? I think you said that you handled them roughly to make sure that they received a nice dose of the uglies.

#38 Lazlo

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Posted 10 January 2007 - 02:53 PM

Well, the Blewit printing was done on my dinner table in the open air and was sitting there for about a week after the print was made. So i'm sure it's pretty bad off with contaminates. As far as the eryngii, Jim gave it to me in a nice package and he knew I was going to use the dry fruitbodies for starting a culture. So he may have used a lot of precautions to help me out with getting a clean culture. Not sure though. Let's break out the nasty arse Cauliflower culture. :headbang:

#39 Lazlo

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Posted 19 January 2007 - 12:51 PM

Alright, so here's the 2 cultures in grain jars. I shook them last night and today they are really looking nice. 2 different types of mycelium as well, which is good because I was concerned with cross contamination. Looking good so far I guess. I find it EXTREMELY hard to believe that at least one of the 2 cultures didn't contaminate. Maybe I got lucky or the salt's doing the trick.

http://mycotopia.net...=1&d=1169230736

We shall see, as here's the nasty arse Cauliflower plate.

http://mycotopia.net...=1&d=1169230312

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#40 golly

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Posted 19 January 2007 - 01:18 PM

Looking good Laz...So all these were hydrated with about a 1% salt solution..?...Sweet..!




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