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Fun with rice


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#1 Tzinacan

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Posted 06 January 2007 - 06:44 PM

Ok, here goes, my very first grow log (I'm excited!). :)

After a lot of reading, I have decided to go with rice.

But given that I am not sure which rice works best, and given that I feel like experimenting a little, as seems fitting with Mycotopia's spirit and traditions, I will test six different commercially available rices. Well, actually, five. My sixth choice is purely for the enjoyment of the amateur alchemist in me, and consists of parboiled corn grains.

So, here's the line up (see picture):
  • #1 Short Grain Rice Parboiled Black "Venere"
  • #2 Long Grain Rice Parboiled White "Patna"
  • #3 Long Grain Rice Parboiled Brown "Thaibonnet" (Organic)
  • #4 Long Grain Rice Parboiled White "Uncle Ben's"
  • #5 Long Grain Rice Parboiled Brown "Thaibonnet"
  • #6 Corn Grains Parboiled "Nattura"
I followed DukeX's Long Grain Rice Tutorial, and filled my 1 pint jars with 1/2 cup rice and 3/4 cup distilled water each.
Right now I am going through the sterilization process, by putting each jar into a pasta cooking pan, for 1 hour steaming.
In the meantime I have prepared six spore syringes from a cubensis spore print (not sure which strain, I am trying to find out).
Overnight the jars will cool and the spores inside the syringes will re-hydrate, and tomorrow I'll inoculate.

I'll periodically post my progress to this thread.

Ciao,
Tzinacan

Attached Thumbnails

  • Line-up-003-before-sterilization_scaled.jpg
  • Line-up-002-numbered_scaled.jpg


#2 spacecake

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Posted 06 January 2007 - 07:16 PM

Nice pictorial Tzinacan...!

I suggest you better boil for 1 1/2 or hours instead of only one hour if you do not have a pressure cooker !

#3 python

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Posted 06 January 2007 - 07:24 PM

GL and thanks for the pics

#4 Guest_Water_*

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Posted 06 January 2007 - 09:39 PM

Cool, looking forward to the updates.

#5 Tzinacan

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Posted 07 January 2007 - 05:05 AM

I suggest you better boil for 1 1/2 or hours instead of only one hour if you do not have a pressure cooker !


Thanks for your concern, spacecake.

I know that 1h seems a little fast, but I did a test run with a jar containing only water, and I noticed that after 20 minutes from the pan's boiling point, the water inside the jar was boiling too - and that's why I assumed that one full hour (from boiling point) would be enough. I hope I am not mistaken here.

Actually, my first idea was to use a steam cooker, but the damn steam cooker couldn't get the water inside my test jar to boil, not even after 90 minutes, so I let go of that thought, and reverted to the good old pasta pan. One thing I want to look into, one day, is using a microwave, as that should be quick, clean and effective.

Anyway, in a few hours I'll inoculate. Wish me luck. :)

Ciao,
Tzinacan

Attached Thumbnails

  • Sterilizzazione-002_scaled.jpg
  • Sterilizzazione-001_scaled.jpg


#6 Guest_vinz_*

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Posted 07 January 2007 - 05:19 AM

good luck!

can u post a pic of what the jar looks like before you innoculate?
im interested to see how it looks like. thanks!

#7 spacecake

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Posted 07 January 2007 - 05:54 AM

I know that 1h seems a little fast, but I did a test run with a jar containing only water, and I noticed that after 20 minutes from the pan's boiling point, the water inside the jar was boiling too - and that's why I assumed that one full hour (from boiling point) would be enough. I hope I am not mistaken here.


Will see..but you cannot garante a fully sterilized jar with boiling.

#8 Tzinacan

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Posted 07 January 2007 - 07:12 AM

can u post a pic of what the jar looks like before you innoculate?
im interested to see how it looks like. thanks!


Sure.

But they will look pretty much the same before and after inoculation. :)
Here are some pictures of an empty jar, and the holes I made.

Ciao,
Tzinacan

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#9 Guest_vinz_*

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Posted 07 January 2007 - 07:58 AM

heh i was jst interested in seeing the moisture content of your grains

#10 Tzinacan

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Posted 07 January 2007 - 08:37 AM

The inoculation process was not as smooth as I had hoped.

I had some trouble due to air inside the jars not allowing me to empty the spore syringes without first relieving pressure, by either widening the injection holes (in two cases, jars #2 and #5), or temporarily loosening the lid to let some air in (jar #4).

I found out about this problem the hard way, when I was injecting jar #2 and after encountering resistence I just decided to apply more force, until, well, the syringe exploded. Ok, not a real explosion of course, but it fell apart, the needle went flying and my face was washed with spores.

Anyway, I went ahead and completed my task. I can't say that I am happy, for I may have compromised my chances of avoiding contamination, but it's my very first attempt and if things don't go well I will just have to chalk it up to experience and try again.

I certainly am not operating under ideal (or even fair) conditions: the environment here is quite dirty due to ongoing renovation, I am not using the most successful of methods, I didn't use a glovebox, I don't have a pressure cooker and I boiled my jars for just 1 hour, I didn't follow the best procedure in making the spore syringes, and now, well - now I have to add a somewhat problematic inoculation to this list.

I wonder if the Mushroom Gods will forgive my noob mistakes and grace me with at least a partial success (I'd be happy if even just one jar would produce a good batch of shrooms), or if they will elect to teach me a lesson, and punish me with total failure.

I am staying positive, and sending a constant stream of happy thoughts towards my jars. :)

Ciao,
Tzinacan

Attached Thumbnails

  • Inoculated-lineup_scaled.jpg
  • Inoculation-003_scaled.jpg
  • Inoculation-002_scaled.jpg
  • Inoculation-001_scaled.jpg


#11 Guest_Water_*

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Posted 07 January 2007 - 09:34 AM

I can't say that I am happy, for I may have compromised my chances of avoiding contamination, but it's my very first attempt and if things don't go well I will just have to chalk it up to experience and try again.


That's the spirit man :thumbup:

I lost two large projects in a few months time and never gave up, never will.

Half the fun of this hobby is experimentation.

#12 Guest_vinz_*

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Posted 07 January 2007 - 09:47 AM

hmmmmm ok basing on ur pictures
there was no hole on the lid of the jar while it was being boiled?
so thats what caused the pressure inside to suck up your syringe
which you had to 'poke' through the lid? coz there was no hole before hand?
and you used 1 syringe per grain jar?
coz that pic has 6 syringes and 6 jars
how many ml are in those syringes? not so much i hope

good luck

btw, good thing that needle didnt hit you in the eye when it broke and spores splat all over your face
be careful

next time put a hole before hand
and stuff it with polyfill or tyvek
and a pc would really save you from contams

#13 Hippie3

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Posted 07 January 2007 - 10:44 AM

I had some trouble due to air inside the jars not allowing me to empty the spore syringes without first relieving pressure, by either widening the injection holes (in two cases, jars #2 and #5), or temporarily loosening the lid to let some air in (jar #4).

I found out about this problem the hard way, when I was injecting jar #2 and after encountering resistence I just decided to apply more force, until, well, the syringe exploded. Ok, not a real explosion of course, but it fell apart, the needle went flying and my face was washed with spores.


:lol:
you need to check out
my > airport < tek.
nice thread/pix so far.
:bow:

#14 Hippie3

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Posted 07 January 2007 - 10:46 AM

... good thing that needle didnt hit you in the eye ...


:lol:
you sound like my
mom.

#15 Tzinacan

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Posted 07 January 2007 - 11:44 AM

hmmmmm ok basing on ur pictures
there was no hole on the lid of the jar while it was being boiled?


Yes, I had poked 5 holes into each lid (see picture I posted earlier), but before boiling I put plastic wrap and aluminium wrap over the lids, so the holes were closed. I thought this would guarantee that no water would get into the jars (which I've read is sometimes a problem). It was stupid not to think that there would be changes in pressure, with the boiling of the water and the cooking and expansion of the grains.

and you used 1 syringe per grain jar?
coz that pic has 6 syringes and 6 jars
how many ml are in those syringes? not so much i hope

Yes, I used a syringe for each jar.
Each syringe holds 5ml (1ml per hole).

good luck

btw, good thing that needle didnt hit you in the eye when it broke and spores splat all over your face
be careful

Thanks, I will. :)

Ciao,
Tzinacan

#16 Tzinacan

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Posted 07 January 2007 - 11:53 AM

you need to check out
my > airport < tek.


Wow, man, this is a great idea!
:cool:

I love this community, it's choke full of competent people and relevant
experience - much more than I would ever have dared to dream.
I'll learn so much here, and I'll have a lot of fun in doing so.

Ciao,
Tzinacan

#17 Tzinacan

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Posted 07 January 2007 - 01:01 PM

Ok, last post for today (probably for a couple of days).
Post-inoculation pictures of all my jars:
  • #1 Short Grain Rice Parboiled Black "Venere"
  • #2 Long Grain Rice Parboiled White "Patna"
  • #3 Long Grain Rice Parboiled Brown "Thaibonnet" (Organic)
  • #4 Long Grain Rice Parboiled White "Uncle Ben's"
  • #5 Long Grain Rice Parboiled Brown "Thaibonnet"
  • #6 Corn Grains Parboiled "Nattura"
Is it reasonable to expect to see some action by next weekend?
I really hope so, as patience is not my strongest virtue (working on it, though).

Have a good evening/night everybody. :)

Ciao,
Tzinacan

Attached Thumbnails

  • PostInocJar06_scaled.jpg
  • PostInocJar05_scaled.jpg
  • PostInocJar03_scaled.jpg
  • PostInocJar04_scaled.jpg
  • PostInocJar02_scaled.jpg
  • PostInocJar01_scaled.jpg


#18 Hippie3

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Posted 07 January 2007 - 01:06 PM

incubation at about 75-80*F
[or 24-27* C]
for at least 3-5 days is strongly recommended
for a quick start

#19 Tzinacan

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Posted 08 January 2007 - 12:59 AM

incubation at about 75-80*F
[or 24-27* C]
for at least 3-5 days is strongly recommended
for a quick start


Thanks Hip.

I will put the jars into a windowed kitchen cabinet later today, and I plan on using a mini electrical stove to keep temperature at 25 C (I need to buy a thermometer). I am wondering, though, if I should do anything about air/gas exchange. Right now my jars are sealed, as I've covered the lid holes used for inoculation with plastic wrap and tape.

Am I correct in assuming that air exchange is not important until the cakes are fully colonized? Once they are colonized, and the risk of contamination will be greatly reduced, I plan on briefly opening and fanning my jars for gas exchange, then un-sealing the holes in the lids and putting the jars uspide down, as described in your Mycro Neglect Tek, in order to try birthing my cakes Invitro (I love simplicity, as long as it doesn't compromise efficiency too much).

Ciao,
Tzinacan

#20 BuckarooBanzai

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Posted 08 January 2007 - 04:32 PM

You will need some fresh air for the jars to finish. Not much, but a little is needed.

I would probably wait until they stall (growth halts) and then crack the lids just a tiny amount. Hopefully, the myc will colonize the rice before any nasties get a foothold.

5mL of spore solution per jar is an awful lot, IMO. Is there any moisture pooling on the bottom?




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