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So Far So Good... invitro


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#1 Laffyn

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Posted 15 January 2007 - 11:21 AM

I started this hobby about 7 weeks ago,
and have had more than my share of trouble
with contams, Trich fer sure.

Somehow I've remained patient
and determined as hell
and now I actually have something to show
for all the sweat and frustration.

These jars were photographed last night,
day 8 (post-inoculation). Dixie strain of Cube.
Noc'd with 2cc per Pint Jar and .5cc per little 'un.
Silicone on the Inject Ports does wonders!

This is an invitro grow attempt. The jar sizes don't matter
much to me at this point, as anything other than
contaminants growing in 'em is a blessing.

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#2 spacecake

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Posted 15 January 2007 - 11:35 AM

How did you made your jars ? (ratio)

If you look closely,then it looks like ,the more you get to the bottom..the wetter/compact the brf seems to get ?!
What do you think ?

#3 Laffyn

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Posted 15 January 2007 - 12:30 PM

I used the PF BRF formula, basically doubled for each Pint Jar and halved for the petite container.

Yeah, the bottom does seem a little mushie/wet, but after PC for about 35 minutes the jars seem to lose some moisture, somehow. I may have to PC them for shorter duration and play with the water:substrate mix. Originally I had PC'd other jars for as long as 60 minutes, but that seemed like overkill as the mix was bone-dry by 2 weeks.

I'm just really happy that I haven't seen any contams yet, crosses fingers and toes.

:pirate:

#4 golly

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Posted 15 January 2007 - 12:46 PM

Looks like the real deal...Congrats Laffyn...

#5 Pedestrian

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Posted 15 January 2007 - 01:06 PM

Welcome to the hobby laffyn!

Normally you don't loose much water when you PC. You might want to check your substrate formula to make sure you are using the right quantities. Also, you should do at least a 60 min PC. It doesnt matter how long your stuff was sitting out. Contaminate spores are there and time doesnt really make them go away. Better safe than sorry you'll soon learn :)

I can't tell for sure, but did you pack them alot when you loaded the jars? Too much packing can leave very little space/air for the myc to grow.


Good luck!

-Ped

#6 Laffyn

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Posted 16 January 2007 - 07:26 PM

I know what you mean about packing the substrate, definitely need to leave room for myc, thanks for the reminder.

I haven't gotten the substrate mix down exactly either, steep learning curve I guess :lol:

I'm going to flip the jars in a couple of days, hopefully get the myc all the way round/ through the Pints. I'll post some more photos then.

Still lookin good... I appreciate the input guys.

#7 Laffyn

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Posted 18 January 2007 - 08:55 PM

Here we are now, day 13 (post noc), much more growth in the last 6 days.

As you can see, I've already flipped the tiny jar, I'm not sure if I should do the Pint's ... concerned about upsetting the myc and causing contams to breed unnecessarily.

Any suggestions?

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#8 Laffyn

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Posted 22 January 2007 - 07:19 PM

Day 17

Not much change in the Pints, just whiter-fluffier mycelium. It's going to be awhile yet before the growth reaches the bottom of the jars, so I've resorted to wrapping 'em with infant sized flannel blankets, trying to keep the temp around 80 or higher.

Oh yeah, the Pints were flipped up, yesterday.

The 1/4 pint has shown better progress, than its sibs, since being flipped 4 days ago.

I'll have pics in the next few days.

#9 Guest_vinz_*

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Posted 22 January 2007 - 07:34 PM

looks good
but it seems to me that you may have some problems having that big one get fully colonized..
good luck!

#10 Laffyn

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Posted 26 January 2007 - 08:20 PM

Day 21

You're right, Vinz... those pints are slowing down. Nothing much new, growth-wise, out of those two.... hmmmmm.

The 1/4 is almost completely colonized, I'd say another 3 -5 days tops and hopefully have pins.

Overall, I'm very happy with this batch, the first so far that hasn't contaminated post-inoculation! Whatever comes now is a bonus.

I've already acquired a dozen half-pint jars and plan to do the PF Invitro by the books in the near future. Certainly make sure that the substrate/verm/water ratio is tight.

here's some photos taken yesterday evening.

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#11 BuckarooBanzai

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Posted 26 January 2007 - 09:47 PM

Cool. Congratulations!

Unfortuantely, a good demonstration of why bigger isn't better in terms of pints vs. half pints. Just give 'em time and keep 'em warm dude, they will eventually finish.

#12 fahtster

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Posted 26 January 2007 - 11:09 PM

you could always cut off the uneaten part if it gets too long to wait. :)

fahtster

#13 ANON

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Posted 27 January 2007 - 03:32 AM

good luck

#14 Laffyn

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Posted 27 January 2007 - 08:55 PM

you could always cut off the uneaten part if it gets too long to wait. :)

fahtster


... that thought has crossed my mind :pirate:

#15 Laffyn

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Posted 31 January 2007 - 08:17 PM

DAY 26

Okay... so the pint jars are pretty much stalled, i'm keepin' em warm with blankets and hopefully they'll cover the entire jar, get back to these guys in a minute.

The 1/4 jar is far more interesting to me right now, as it has been fully colonized for about 3-4 days. There's something like one or two tiny wormy bits growing on the side of the cake just below the lid, where the dry verm meets the sub, but the view is obstructed enough that I can't tell if its good - as in, yes, these are primordia or even mushies - or bad - as in some really funky contam.

I also tried taking some photos with the cam but I can't get a clear enough shot.

I would have aerated the lil pint today but I haven't any clean space nor a proper container to do it in, have to wait till tommorow, which is okay as I've been more than busy in all other aspects of my life!

Once I get the dry verm out and have a nice clean space ready, I'll get some photos up.

fingers crossed that ole dixie won't fail me.

(I'm gonna keep the pints going for as long as I can.... who knows, maybe I'll decide to liberate 'em, I've got a hammer around here, somewhere.)

:pirate:

#16 Laffyn

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Posted 01 February 2007 - 08:48 PM

DAY 27

Opened all jars, tonight... dunno why I did the PINTs, but hey I had a hammer and Smashed away. Cut the buggers in a quarter down through the snowy mycelium portion only. I doubt that either one will make it free of contams, but in their new clean half-pint homes hopefully they can at least completely colonize. We'll see...

On the other hand, the 1/4 Pint is in good shape (see photos below):

Yup there's a mushie alright, a skinny little worm. Cool! My first MM. Wonder what it tastes like....hmmm. I didn't want to remove it from the cake, just happened to fall off when I was removing the verm barrier, darn.

Gonna watch that jar closely, any signs of it drying out and blueing and will have to dunk it.

I really wasn't expecting to get any shrooms this batch, so I'm pretty happy with this meager showing.

Thanks for all your help, gang.

Next grow will be dixie, again, this time in six half-pint jars. I'll start a new thread once everything is underway.

:pirate:

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#17 Laffyn

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Posted 03 February 2007 - 10:35 AM

DAY 29

All the cakes look a little thirsty, I've also noticed some blueing/bruising? (probably due to over-handling, I'm sure that focefully breaking the old PINT jars the other day didn't help)

So this morning, around 9am I took a medium sized tupperware container filled it 3/4 full with distilled water (no bleach), placed the cakes inside and added a smaller square shaped tupperware container about a two-cup holding capacity. Placed it straight up over the cakes, this seems to keep them under the surface nicely.

Don't suppose there could be a problem with it pressuring the cakes too much? I'm thinking about bruising or any other damage that could come from this method.

Plan is to check the cakes in 8hours, and if not hydrated enough (curious how to tell, wetness and heaviness I guess) then push on for another 4 hours till next check.

Have some photos and more later.

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#18 Laffyn

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Posted 03 February 2007 - 04:59 PM

Not much difference so far, check again around 9pm.

#19 Laffyn

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Posted 04 February 2007 - 12:09 PM

DAY 30

Yesterday, I got caught up on the phone with a friend and totally forgot about checking the cakes in the fridge till about 10:30pm. Checked then and seemed hydrated, so I patted em dry with paper towel and returned them to clean jars to sleeep the night away.

This morning at about 9:30am have given em some nice nature-mimic-ing light from my desktop Sun Lamp. They get about an hour on and an hour off of semi-direct lighting. I'm too cheap to buy a timer and I don't want to play with the lighting any other way for this grow.

They seem okay, lotsa moist fluffy white mycelium, in fact I think there may be some action, like 'pinning' happening, at the moment.

The inner part of the jars is saturated with moisture making it difficult to differentiate bits of verm from anything else browny-gold in color.

I'm gonna hold off on taking some pics till this evening... give the children a chance to do their thing, peacefully.

:pirate:

#20 Jetson

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Posted 04 February 2007 - 06:04 PM

Did the pint jars ever fully colonize? Maybe you could make some sort casing with them? Or are you doing strictly invitro?




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