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#21 Boombloom

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Posted 04 February 2007 - 12:02 AM

Maybe i missed somthing but this thread makes no sense to me.
How do you just "find" spores lying around outside?
And why would you try to cultivate some junk that you just picked up...I mean theres really no way to just single out one type of spore to grow (I assume your knowledge and equipement is not as professional as need be) It sounds to me like you are growing a whole bunh of different crap and could be risking your own health. If you really just want to practice to make sure you are capable maybe you should try cloning a mushroom making a spore print or somthing. Maybe I am the one with the misunderstandig here but from what i read there seems to be no logic in what you are doing. Growing random cultures can be life threatening.

#22 BaconSquishy

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Posted 05 February 2007 - 03:45 PM

Well i found a sort of puff ball type thing which when popped produced a green gas full of what i assumed to be spores. Possibly trich spores? but as you can see the cake isnt green. Also does anyone have any instructions on how to clone and can this be done with any mushroom even ones found in supermarkets or must it be fresher than that? Also which part do you have to cut to clone? The cap or what?

#23 Boombloom

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Posted 07 February 2007 - 01:23 PM

You need a sterile piece from inside the stem and yes you can clone a fresh mushroom from the grocery store. Look into cloning archives..I belive reverend trips has a nice method thats fairly easy.

#24 BaconSquishy

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Posted 07 February 2007 - 08:38 PM

Thank you thank you ill look into it.

#25 BaconSquishy

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Posted 25 February 2007 - 12:42 PM

Ok so i followed the advice given in this thread and clone 4 mushrooms i got for the store. Please look at the pictures and comment. The first is from 2 days ago and the 2nd is from today.

Attached Thumbnails

  • Shiitake Day 4.jpg
  • Shiitake Day 2.jpg


#26 Hippie3

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Posted 25 February 2007 - 02:08 PM

contam'd by bacteria i'd say, rather massively, too.
you do have a glovebox / sterile work area to
make the transfers to agar ?

#27 BaconSquishy

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Posted 25 February 2007 - 03:21 PM

ok so just cut that part out and put it in another thing of agar?

#28 Hippie3

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Posted 25 February 2007 - 03:23 PM

you could try that...

#29 Bobcat

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Posted 25 February 2007 - 09:32 PM

Don't give up!

#30 BaconSquishy

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Posted 25 February 2007 - 09:56 PM

Lol thank you im trying not to. It appears that some of the agar isnt yet colonized by the contaminant so should i let it grow until both have grown to their fullest potential and then transplant or should i now?

#31 BaconSquishy

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Posted 26 February 2007 - 07:54 PM

Day 5 picture update. Seems the intruder spreads faster than the mycellium. Think it will all be ready to transplant in a couple days.

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  • Shiitake Day 5.jpg


#32 Hippie3

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Posted 26 February 2007 - 08:31 PM

i surely would not wait,
you should take a tiny piece of mycelia
as far away from that slimy bacteria as possible
for immediate transfer,
proly will need a few more transfers after
b4 it's finally clean.

#33 BaconSquishy

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Posted 27 February 2007 - 05:19 PM

There is still hope! The pictures i had been suplying were only of one that i would open up each day to track progress while keeping the other ones sealed so that if any got contaminated it would most likely only be that one. Well i looked at the other ones briefly today and two have bacteria worse than the other but there's one that looks like the diagram attached with no bacteria. My question is when can i cut a piece off to inoculate a jar of substrate, does the entire container have to be colonized? Also, which piece do i cut to move to a clean dish for the contraminated ones, any part of the mycellium or is there a specific area/part?

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  • untitled.JPG


#34 BaconSquishy

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Posted 05 March 2007 - 06:34 PM

Finally took a picture. Here it is:Posted Image
I transplanted today. How long do you think i have to wait before it shows some signs of growth?

#35 BaconSquishy

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Posted 06 March 2007 - 09:14 PM

Anyone? Any estimates?




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