45 replies to this topic

[chimp]

Very interesting Golly.

[izzbizz]

Hmm.. foaf didn't think one could propagate Cubes from dried flesh. Go figure. Also.. is it possible that the dried flesh would still contain viable spores (if stems & caps were stored together) ?

[golly]

Even though all the shrooms were harvested b4 the caps opened ,there could be some spores on the samples...
There was no peroxide dunk, so any spores would be viable...

[BuckarooBanzai]

When you harvest a big'un, put the stem in a sealed glass tube. You can tear that sucker apart and pull sterile tissue out of it that will hydrate and grow on agar quite nicely.

You can clone dry tissue just like fresh, it just starts a good bit slower. Much harder to "clean up" a dirty sample on agar. Lots of transfers requried unless you have clean internal tissue to start with.

[Bio_DUD]

Golly--how did the "cannabalistic" using stem butts as precursor increasers in the substrate work out? I'm sure you had fun researching it.

[golly]

Well Bio the third generation is coming up shortly...The first two were quite positive..

This thread is simply exploring the possbilities with cardboard , there is no attempt at sterility here..what ever turns out to grow well in these experiments, should also work the best under more sterile conditions which can be done quite easily...
The easiest way to transfer from cardboard to grains or LC, is to grab a colo'd section and card together with a pair of tweezers and tear it out ..
Trying to scrape the myc off doesn't work too well...The Cboard becomes quite soft and tearable after a week of incubating...

[Bobcat]

This is what happens when you add too many nutes in a non sterile environ..
The left side clone has a lovely cobweb bloom...heh..
This is how Golly gets his jolly's...

I wonder if Lazlos salt solution added into your design would be helpful?

[joystik]

If sterility is not an issue here would it be practical to use the colonized cardboard for a LC? Would it get contaminated?

Im a little confused here.

Joy.

[golly]

No these samples are not sterile, I'm just doing this way first because they are easier to work with and photograph..
It's easy enough to get near sterile internal stem tisue as Bucky alluded to.

But i proly could take a small piece from the growing edge and transfer to sterile cardboard, grow that out , then use a small portion of that for whatever...
If i can get vigorous growth from the dry samples - then that would be a good method...With fresh mushrooms it's alot simpler just to go direct from
stem to C.board to grains or LC...

Essentially the card step is like a Ghetto agar plate...:)

BC ...I have been thinking about the 1% salt solution and plan to do a few...thnx...

[Hippie3]

i'd think a good avenue of approach, nute-wise, would be the
water used to wet the cardboard.
perhaps some castings tea would not mold yet give an extra boost ?

[golly]

Just made up a salt/karo dip... At a 1% consentration and halved that again
.5% for the third piece.
More dried stem fragments were used and will also drop some spores on the corners..
The two triangles are the 1% ,the square is the half strength...

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[Killjoy]

keep the variations coming, your amusement is shared!!!


Been thinking on cardboard since I saw that '99 Stamets lecture someone graciously posted awhile back.

[izzbizz]

really interesting stuff. let us know if any of the dry pieces on cardboard develop into strong mycelium.. :0)

[BuckarooBanzai]

Do you do anything at all to moisten the dry tissue before putting it on the cardboard? A drop or two of water (or maybe even H2O2) might help things along.

[golly]

The dry pieces are very good at wicking moisture off the cardboard ,it only takes a few hours to hydrate + it should pull in some of the nutrients too..

I will try some manure tea versions ,I have worm and horsey poo 2 play with.

Running low on tupperware though...Heh....

[golly]

A quick update ..The original CBoard clone is still growing well but has really
avoided the two zones where additional nutes were added..

A small section from the growing edge, which was transfered to a spawn jar
has continued expanding with good rhizo type growth..pictured below...

All the dry tissue samples are showing no growth...They appear to be overly wet..Think i will re-do these...

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[shobimono]

How did you transfer the small piece? flowhood? glovebox? open air?

What is the spawn in that jar? Looks like verm and coir?

[golly]

Transfered in open air... The only things that were sterile, was the tweezers and the spawn jar...Proly a bit of luck ...This should be more sterile for serious cloning....

[golly]

Here's another test, this time the Card soaked in 1% karo .05% salt solution

The bottom row is Cube, Bluefoot and Agaricus myc ..After 8 days growth is very limited at best..

The middle row is dried Panaeolus flakes and top row, dried Cube stems..

The only thing of note, is the suppression of mold growth on the dried samples ,which would normally be covered by now..

So far i have not been able to regenerate any dried tissue...So i have to ask,
When is the last time somebody was able to regenerate on Agar or any other media..?
The only time i have regenerated from dry material, was a stembutt which also had a bit of dessicated mycelium attatched..

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[cheshire]

i took a piece of ryr that had fallen out a colod jar that was about a month old and didnt look like it had myc or anything, left it in a sharpener and now the whole things colod :p