Carboard clone ideas
Posted 27 January 2007 - 03:25 PM
Posted 27 January 2007 - 09:23 PM
There was no peroxide dunk, so any spores would be viable...
Posted 28 January 2007 - 01:05 AM
You can clone dry tissue just like fresh, it just starts a good bit slower. Much harder to "clean up" a dirty sample on agar. Lots of transfers requried unless you have clean internal tissue to start with.
Posted 28 January 2007 - 10:33 PM
Posted 28 January 2007 - 11:14 PM
This thread is simply exploring the possbilities with cardboard , there is no attempt at sterility here..what ever turns out to grow well in these experiments, should also work the best under more sterile conditions which can be done quite easily...
The easiest way to transfer from cardboard to grains or LC, is to grab a colo'd section and card together with a pair of tweezers and tear it out ..
Trying to scrape the myc off doesn't work too well...The Cboard becomes quite soft and tearable after a week of incubating...
Posted 29 January 2007 - 06:13 AM
This is what happens when you add too many nutes in a non sterile environ..
The left side clone has a lovely cobweb bloom...heh..
This is how Golly gets his jolly's...
I wonder if Lazlos salt solution added into your design would be helpful?
Posted 29 January 2007 - 08:51 AM
Im a little confused here.
Posted 29 January 2007 - 09:41 AM
It's easy enough to get near sterile internal stem tisue as Bucky alluded to.
But i proly could take a small piece from the growing edge and transfer to sterile cardboard, grow that out , then use a small portion of that for whatever...
If i can get vigorous growth from the dry samples - then that would be a good method...With fresh mushrooms it's alot simpler just to go direct from
stem to C.board to grains or LC...
Essentially the card step is like a Ghetto agar plate...:)
BC ...I have been thinking about the 1% salt solution and plan to do a few...thnx...
Posted 29 January 2007 - 10:26 AM
water used to wet the cardboard.
perhaps some castings tea would not mold yet give an extra boost ?
Posted 29 January 2007 - 01:15 PM
.5% for the third piece.
More dried stem fragments were used and will also drop some spores on the corners..
The two triangles are the 1% ,the square is the half strength...
Posted 29 January 2007 - 02:17 PM
Been thinking on cardboard since I saw that '99 Stamets lecture someone graciously posted awhile back.
Posted 29 January 2007 - 06:01 PM
Posted 29 January 2007 - 08:13 PM
Posted 29 January 2007 - 08:48 PM
I will try some manure tea versions ,I have worm and horsey poo 2 play with.
Running low on tupperware though...Heh....
Posted 03 February 2007 - 12:47 PM
avoided the two zones where additional nutes were added..
A small section from the growing edge, which was transfered to a spawn jar
has continued expanding with good rhizo type growth..pictured below...
All the dry tissue samples are showing no growth...They appear to be overly wet..Think i will re-do these...
Posted 03 February 2007 - 02:54 PM
What is the spawn in that jar? Looks like verm and coir?
Posted 03 February 2007 - 03:28 PM
Posted 16 February 2007 - 10:15 AM
The bottom row is Cube, Bluefoot and Agaricus myc ..After 8 days growth is very limited at best..
The middle row is dried Panaeolus flakes and top row, dried Cube stems..
The only thing of note, is the suppression of mold growth on the dried samples ,which would normally be covered by now..
So far i have not been able to regenerate any dried tissue...So i have to ask,
When is the last time somebody was able to regenerate on Agar or any other media..?
The only time i have regenerated from dry material, was a stembutt which also had a bit of dessicated mycelium attatched..
Posted 16 February 2007 - 10:18 AM