45 replies to this topic

[golly]

I'm not sure what u mean Chesh...Whats ryr and a sharpener..?

[Lazlo]

Here's another test, this time the Card soaked in 1% karo .05% salt solution
The bottom row is Cube, Bluefoot and Agaricus myc ..After 8 days growth is very limited at best..
The middle row is dried Panaeolus flakes and top row, dried Cube stems..
The only thing of note, is the suppression of mold growth on the dried samples ,which would normally be covered by now..
So far i have not been able to regenerate any dried tissue...So i have to ask,
When is the last time somebody was able to regenerate on Agar or any other media..?
The only time i have regenerated from dry material, was a stembutt which also had a bit of dessicated mycelium attatched..

It's rather easy to do. The last one I did was a dry Morchella elata. It fired right up. So far i've been successful with all of my attempts that include, Angle's Wings, Morchella and Hen of the Woods. Hen of the Woods was done on cardboard as a matter of fact.

Oh, and potentially Pleurotus eryngii as well. Maybe more, I can't recall.

[BuckarooBanzai]

My FOAF "brought back" (and fruited) internal tissue from a thick Hillbilly stem about three months ago. The stem was dried standard process to cracker dry and then thrown in a big glass screw top vial.

A little over a year later, the step was popped out, torn in half and a little sample of clean internal tissue was smushed/smeard onto a couple of agar plates. It came right back and took over the plate.

Truth is, FOAF was really fucking amazed that it worked.

[golly]

Thanx for the imput guys..The remaining option for C.Board then is to control the bacteria which must be preventing new growth..I will try a pretreatment to level the playing field a bit...

[Hippie3]

good luck!

[Lazlo]

Golly, something else. I've found that NOT hydrating the specimen seems to work better. A really small one seems to take off better for some odd reason also. Good luck.