18 replies to this topic

[Leary's Ghost]

i don't know how others do it, but i have found the flexible little art knives to be perfect for folding the edges of the prints over. it makes a nice clean edge, they can be sterilized w/ iso, and i never have to touch the print w/ my fingers. i do it in a draft free room after sprayng oust and wearing gloves, hooded tyvek jumpsuit, and face mask. my arms are exposed in the pic and the surface is butcherblock but thats cuz this was for illustration only. usually my arms would be covered, and the work surface is white laminate.

can you dig it?

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[waylitjim]

I can dig it. Nice pictorial. :)

But I'm just curious, why fold them at all ?
Why not slide the foil in a small ziplock so you can see the print?
This way, you can just squirt sterile water in the bag, rub off the spores and withdraw the spore solution with the same syringe. It makes the process of making a syringe very simple and clean. This is how I do mine, but everyone's got their own style.

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[siam_jim]

kool nice work there, the knives looks very handy for other things too.
i find the fold works better for me, as i don't want to use all the print in one settings so i just scrape what i need in one of them ziplocks and do the syringe that way.

siam

[Lazlo]

Damn! I'll take a print from you any day of the week. If you're folding's so thorough, i'd love to see how clean your specimen is.

Moving to the Gallery.

[siam_jim]

I think this needs to be archived…yes?

Archive to the vault
Spores [Microscopy & Shroom Biology]


siam

[Leary's Ghost]

I can dig it. Nice pictorial. :)

But I'm just curious, why fold them at all ?
Why not slide the foil in a small ziplock so you can see the print?
This way, you can just squirt sterile water in the bag, rub off the spores and withdraw the spore solution with the same syringe. It makes the process of making a syringe very simple and clean. This is how I do mine, but everyone's got their own style.

and i am diggin' your new avatar waylit. very psychedelic man!

i like the way you do it. it would make syringes easy and being able to rub the print in the bag to losen the sporage would be bitchin.

i am just afraid that the back of the foil might get contamed by whatever surface its on. even though its sterileized w/ iso, i get spooked any time anything touches the foil. also, putting the print into the bag should be done in a GB right? GB's hurt my back.

i cover the caps being printed and the foil is folded over like 130 degrees. after the caps drop their sporage, i slowly tilt the cover up just enough to skewer the cap w/ a syringe needle removing it from the foil and set the cover back down. i allow the prints to dry under the cover w/ the foil left still open about 50 degrees. then i tilt the cover back open w/ my space suit on and fold the top piece of foil flat before i take it out from under the cover so nothing can fall on the print.

i am prolly being to paranoid about contams.

these pics show the cover but the foil is laying open flat (180 degrees). since then i have been leaving the foil folded up 90 to 130 degrees so i can cover the print faster by just pressing down on the flap w/ the knife.

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[Leary's Ghost]

I think this needs to be archived…yes?
Archive to the vault
Spores [Microscopy & Shroom Biology]
siam

wow! my first archive!
thanks siam:bow:

[Hippie3]

i'm with waylit-
open-faced is the way i fly.
http://mycotopia.net...57&d=1171696233

i've found it difficult to un-fold prints
when i'm wearing gloves and stuff is wet with lysol, etc.
tape makes that even worse.
the ziplocks one does not even need to open,
i just rip off the end, squirt in a few cc of sterile water
rub a little and draw it back up, now dark purple,
for dilution in more water for however many syringes needed.

[izzbizz]

waylit.. what's a good (non-suspicious) place to get those small baggies for prints?

[chimp]

Try a arts and crafts store...they use em to put beads in.

[waylitjim]

Exactly...i get em at Michaels Crafts.

[shobimono]

I can dig it. Nice pictorial. :)
But I'm just curious, why fold them at all ?
Why not slide the foil in a small ziplock so you can see the print?
This way, you can just squirt sterile water in the bag, rub off the spores and withdraw the spore solution with the same syringe. It makes the process of making a syringe very simple and clean. This is how I do mine, but everyone's got their own style.

How do you use "part" of a print? This method seems kinda all or nothing imo.

I mean sure you can reach inside and cut the print in half, but then what do you do with the other half?

If you fold a print in foil, you can cut a piece out without unfolding it. You can get many "uses" or "attempts" out of a print.

You are also using a whole print for a single syringe.

Please don't say "well you can water them down".

I know that, BUT I have not seen that mentioned in a single post about using the print in baggie method. The way they are all written, you use a whole print for a single syringe.

I find this intersting because some of the same people advocating this method will also state that dark syringes cause overcompetition and decreased colonization.

[mrpanda]

To use part of the print, open the baggie in a glove box and use a flamed and iso'd inoculation loop to scrape spores from the print into a shot glass filled with water to make a syringe, or streak the loop across agar to make a culture. When you have finished with it just reseal the baggie this way one print will last you for a long time.
http://en.wikipedia....noculation_loop

[Hippie3]

You are also using a whole print for a single syringe.

Please don't say "well you can water them down".

I know that, BUT I have not seen that mentioned in a single post about using the print in baggie method. The way they are all written, you use a whole print for a single syringe.

not so.
you do get a single syringe full of concentrated solution, true,
but here's a trick i learned from hillbilly -
make up a pint jar like one of his refill kits,
about 300 ml of water, sterilized in PC as usual,
twin silicon injection ports in the lid.
place airport thru one to keep air pressure equal.
take a fresh sterile syringe, draw up several cc of the water
inject your ziplock print, rub it all off, suck it back into syringe
then inject that back into the pint jar,
voila! enough spore solution to make 25-30 syringes.
if the print was light or small
just repeat process using additional prints into same jar
until desired concentration is reached.
:bow:

[izzbizz]

arts & crafts, ey? hehe. thanks for the info

[duncan]

I like making what I call spore juice. I did this last year. i was making up pints of different strains similar to what you describe hillybilly doing. I would pc my jars and utensils. I would boil what ever amount of water I wanted cool water and place in pc'ed jars. once cool I would open the jars with the cool water and scrap with a sterlilized knife the spores in to the water and place the lid back on give it a few good shakes and then close it up and toss it into the fridge. Labeled and I was good to go for awhile.

[mushit]

I like the "Master" syringe as well.

If you just want one syringe from this master just squirt a few cc's from this concentrated syringe into a shot glass. Then take another syringe with the balance of the volume needed to fill it and squirt it into the glass. Stir and suck it back up into the syringe.
The master can be stored until the next time.

[Hippie3]

cf. http://mycotopia.net...ead.php?t=20497
don't want to drag this thread off further
but i added pix to copies of my posts above in linked thread
more pix to come

[Leary's Ghost]

arts & crafts, ey? hehe. thanks for the info

"ULINE" also has a nice selection of bags. all different sizes and thickness.