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B+ LC


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#1 coastalite

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Posted 17 February 2007 - 11:03 AM

This is a LC at 10 days old of my FOAF's syringe of B+ from Hillbilly! Thanks Hillbilly.

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#2 Hippie3

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Posted 17 February 2007 - 11:20 AM

damn, that's some good looking growth there
http://mycotopia.net...66&d=1171728105

#3 coastalite

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Posted 17 February 2007 - 11:33 AM

Thnx hip! FOAF couldn't have done it w/out all the help from you guys.

FOAF used a LC Tek of boiled and filtered potato H2O mixed w/light karo syrup and spring H2O and PC for 30 min.

#4 Hippie3

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Posted 17 February 2007 - 11:41 AM

that pic is so nice
i will edit topia's glossary entry for LC to include it.
:bow:

#5 coastalite

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Posted 17 February 2007 - 12:23 PM

thnx, it's an honor. that is some sick-ass growth fer sure though. The mycelium looks like the "Crystaline Entity" in that one star trek episode. FOAF just set the jar atop a 6volt big daddy flashlight (for lighting) but couldn't get the greatest picture resolution with his 2.0 megapixel camera. will post more if FOAF can get some clearer pics.

edit: ok FOAF got more pics. 1st one is the same B+ LC at 10 days old. 2nd one is TX LC at 10 days old. 3rd pic is colonization growth of the two LC's at FIVE days. Again, thank you Hillbilly!

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#6 coastalite

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Posted 27 February 2007 - 11:38 PM

My cat used a LC of B+ and injected 12cc's per jar. This is only 4 days old.
Wide-mouth pint jars using cooked brown rice and a top verm barrier. How does she look?

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#7 the_chosen_one

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Posted 28 February 2007 - 12:06 AM

:eek: 12 cc's per jar! i don't think it had any choice but to grow :lol:

nice work! :eusa_clap

#8 AUS ROTTEN

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Posted 28 February 2007 - 12:57 AM

lol i agree with [user]the_chosen_one[/user]- they had no choice:eusa_thin

#9 Pscilencio

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Posted 28 February 2007 - 03:55 AM

Wow.

#10 shroomtastic

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Posted 28 February 2007 - 04:14 PM

lots of cc's! couldn't u use like half that? guess it would take way longer tho. my buddy used 1cc per half pint jar.

#11 boots420

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Posted 28 February 2007 - 05:06 PM

gee golly that was a lot of liquid your cat shot into those jars.

#12 coastalite

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Posted 28 February 2007 - 06:03 PM

my cat has more lc than it knows what to do with. since this is just cooked brown rice with a top layer of verm, my cat thinks you can get away with shooting a lot more cc's. the cooked rice just soaks it right up like a sponge and colonization is really fast. Have you ever seen a wide-mouth pint jar colonize that much in just 4 days?

btw, how the hell do you insert a picture?

#13 SquallRealm

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Posted 28 February 2007 - 06:11 PM

4 Days!!! :eek:
Do you know what the LC consisted of?

#14 shroomtastic

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Posted 28 February 2007 - 06:28 PM

hell no thats fast! :bow: i guess if u got lots who cares right! better than waiting a month.

#15 the_chosen_one

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Posted 28 February 2007 - 07:05 PM

yer sure it's myc right? :eusa_eh:

#16 coastalite

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Posted 28 February 2007 - 07:22 PM

4 Days!!! :eek:
Do you know what the LC consisted of?



My cat used this recipe:

Take 4 cups of spring water and add to a medium sized pan that has a tight lid. While you are bringing it to a boil, take a couple of medium sized potatoes (any variety – russet were used in this tek), rinse them off good and dice them up with the skin on and add them to the boiling water. Bring it back to a boil, then cover, and then reduce the heat to low/medium-low. Using an electric stove in this tek, the temp was reduced down to simmer at about a 2.5 heat setting. Simmer for 20 minutes. Remove the lid and turn off the heat and place the pan on one of the other burners to cool down a bit.

Now, we’re going to filter the potato water through a coffee filter and into a large measuring cup. For this tek a 4-cup capacity Pyrex glass measuring cup was used. If you have a coffeemaker, you can use the basket and place it over your large Pyrex or bowl. Put a filter in and pour some potato water into the coffee filter. A fork was used to keep the lever on the basket pushed down to allow the water to be filtered through. To speed up the process, you can pour about an inch at a time into the filter and squeeze the water out by hand. You do this by lifting the coffee filter up around the edges, crimping it together and twisting it up. As you are twisting, squeeze the filter gently and the water will seep out. You will have to repeat this process using a new filter each time until you have 2 cups of potato water. Be careful though, that water is hot! We’re just trying to filter most of the potato particles out of the water.

OK, now we have our potato water that’s rich in nutrients and dextrose, which the mycelium will love you for. At this point you can either eat or discard the potatoes. Take 5 Tablespoons of Light-Karo syrup or light-corn syrup and mix it in really well into the 2 cups of potato water that you just filtered. Next, add 6 cups of spring water and stir it in really well. A gallon of spring water is about $1 at the grocery store. Go ahead and splurge and get 2-3 gallons.

For this tek, 4 wide-mouth pint jars were used with your standard four 3/8th holes drilled per lid. Fill each jar halfway with the karo/potato water solution that you just stirred up. You’ll have some leftover potato/Karo water, so you can either use it in additional jars or throw it away. This recipe will yield 8 cups of LC. Whatever size jars you use, fill them each up half-way with your LC. If you find you don’t need 8 cups worth, you can halve the entire recipe. Also, if you have a bag of marbles, wash and rinse them and add about 3-4 per jar. They’ll help break up the clumps of mycelium when it comes time to swirl the water around in the jars. The marbles are optional though. Cover the jars with the lids rubber-side down and take 2 layers of Tyvek that you will have already cut up from some USPS envelopes and place them over the lids. Then screw the bands on till they’re snug but not too tight. Then place 2 layers of aluminum foil and crimp it down tightly over the lids and sides of the jars. Before you put the jars into the PC, you might want to crumple a few balls of foil and place them on the bottom of the PC, then put your rack in on top of the balls of foil. This helps to keep the jars off the bottom of the PC. You may also want to place a washcloth on top of the rack and then place the jars on top of the washcloth. This will help prevent the Karo from getting too caramelized (yellow) but if it does, don’t worry about it, it’ll still work great.

PC for 30 minutes and let the PC cool on its own. Once the locking pin drops and there's no more pressure, take the jars out and remove the foil and tighten the lids. You may need to use a clean paper towel to dry off any moisture that formed on top of the Tyvek lid. Let the jars cool for several hours till they are at room temperature. Inoculate with 2cc's of a multispore or LC syringe and incubate anywhere between mid 70's to low 80's. Swirl the jars at least once a day if you can. In a few days you should have yourself some kick-ass LC. In about 10-14 days it will look like the attached pictures and you will have so much you won't know what to do with it all. When it comes time to suck up the LC into your syringes, do it in a glovebox and just put a piece of breathable medical tape over the injection hole each time you make a hole. Wipe the Tyvek layer with a wet 91% isopropyl alcohol soaked paper towel. Be sure to flame and wipe the 16 gauge 1.5" needle and then simply insert the needle through the Tyvek and through one of the four holes you drilled in the lid. Swirl the jar around and tilt it so the tip of the needle is submerged and then draw back the plunger all the way. My next post will be the rice recipe that you can use.

Now you can see why my cat used 12cc's of LC per pint jar.

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#17 coastalite

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Posted 28 February 2007 - 07:36 PM

yer sure it's myc right? :eusa_eh:



does a one-legged duck swim in circles?

LOL!

#18 coastalite

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Posted 28 February 2007 - 07:52 PM

OK, once you get your syringes filled up with this LC, try this simple rice tek and you too should get similar results in just a few days of colonizing. It should also be mentioned that you'll probably want to use 16 gauge needles 1.5" long. 18 gauge is too skinny to suck up this fat-ass mycelium into your syringes. While you're at it, you might as well go with 30cc or 60cc syringes. We're gonna use 12cc's of LC per WM pint jar.

Preparing the rice. The rice used is a 2 lb. bag of Food Lion brand long grain natural brown rice at about $1.40. Get a few bags. Use WM Pint jars with 3/8th holes drilled in the lids. Drill 4 holes per lid.

Measure out 3 cups of rice and dump into a large pan that has a tight lid. Measure out 5 cups of bottled spring water and pour it in the pan with the rice. Cover and bring to a boil. Once it reaches a fast boil, reduce the heat to around 2.5 (low/med low) and time it for 40 minutes. Do not open the lid at all during the 40 minutes. Once the 40 minutes is up, turn off the burner and sit it on one of your other burners to cool down and open the lid and fluff it up. Cover it back up and time for another 10 minutes to let it cool a bit and evaporate any remaining water. Then fluff it up again. The rice should be nice and sticky and fluffy with no more water in the pan.

While the rice is cooking, go ahead and get your WM Pint jars ready. Wash the lids, bands and jars and thoroughly rinse them and turn them upside down to drain out on a clean paper towel. Then take a fork and fill one of the jars up to the first thread. As you are filling the jar, be careful not to compact the rice down too much. Give it a couple of side to side shakes if there are any large pockets of air. Once it’s filled to the first thread, take a clean paper towel and wipe around the inside top of the jar. Now, take your fine/medium grade vermiculite and fill the jar the rest of the way and level it off. Now place one of the lids rubber side down. Next, place 1 piece of Tyvek on top of the lid. Screw the band on a bit snug but not too tight. Cover tightly with two layers of foil. Place them into the PC and this time cook for 45 minutes. Don’t start the timer till the jiggler starts wobbling. Once it’s finished, remove the PC from the burner and let it cool till the pin drops which should take about 20 minutes or so. While the PC is going for 45 minutes, start another batch of rice. Repeat the process till you have all the jars done. When you remove each of the jars from the PC, take the foil off and reuse it for the next batch. Then, wipe the Tyvek dry with a clean paper towel. Remove the band, and carefully lift the Tyvek off the lid (and re-use for the next batch) leaving the lid still in place. The vermiculite layer should be dry and will act as a protective barrier to keep the contams out (hopefully). Then, screw the band back on tight. Do this for each jar. Let the jars cool for several hours before inoculating them. If it makes you feel better, you can take an extra precaution and put a loose piece of foil over the lid to prevent contams but still allow them to breathe and cool down. You don’t want the foil to trap heat and cause condensation to build up in the foil and drip onto your dry vermiculite barrier.

Three cups of rice and five cups of spring water will be enough to fill 5 WM Pint jars and have enough leftover for a healthy snack.

When the jars have cooled for several hours or overnight and are at room temp, go ahead and remove the loose foil from the jars and place the jars in the GBox. For this tek, we are going to use 1 full 10cc syringe of our LC per WM Pint jar. Remember though that each syringe when full to capacity is probably more like 12cc’s. So, you’ll go ahead and inject about 3cc’s per hole, per jar. Place your 91% soaked alcohol paper towel and 1 labeled syringe per jar in the GB along with your lighter. Secure the lid on the GBox and get ready to inoculate. Flame, wipe and shake the needle. Hold one of the jars and tilt it at an angle and insert the needle tip as far in as it will go, keeping the tip against the side of the glass. Inject 3cc’s per hole. On the last 3cc’s try injecting it in at full force. It will blast down around the side and to the bottom, which could be why the bottom of each jar colonized so quickly. When the syringe is used up, cap it, put it aside, and move on to the next jar. You might want to gently shake each jar (side to side - not up and down) after inoculation to redistribute the vermiculite layer around a bit in case the needle left a hole in the barrier. Cut some squares of masking tape and stick one on the top of each lid but be careful not to cover any of the holes. Label the strain and date on each piece of tape with a fine point marker or a pen. Repeat the process till all your jars are done. Incubate them somewhere where the temp is mid 70's to low 80’s.

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#19 the_chosen_one

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Posted 28 February 2007 - 10:21 PM

does a one-legged duck swim in circles?

LOL!


:lol: i'm glad you took that well :bow: . this is turning into a nice post and write up! it may a bit early, but i'm gonna tag it now...

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