
Cloning Pins Directly to Agar
Started By
Hippie3
, Feb 24 2007 09:38 AM
10 replies to this topic
#1
Posted 24 February 2007 - 09:38 AM
#2
Posted 24 February 2007 - 11:03 AM
the pix are nice :D
#3
Posted 24 February 2007 - 11:08 AM
:loveeyes:
Wow thats cool thanks for sharing hip! Is the one dried?
Wow thats cool thanks for sharing hip! Is the one dried?
#4
Posted 24 February 2007 - 11:16 AM
i do not see dryed tissue here
#5
Posted 24 February 2007 - 12:17 PM
fresh pins
#6
Posted 24 February 2007 - 02:14 PM
Hi Hip, what agar-nutrients do you typically use for plates like this? Is is potato-dextrose?
Thanks
Thanks
#7
Posted 24 February 2007 - 06:50 PM
Doesn't the mycelium start growing from the stem butt? I see in those pics some of them were sliced open?
#8
Guest_vinz_*
Posted 24 February 2007 - 08:04 PM
mycelium grows anywhere from a fresh mushroom.. the reason why people cut the inside to get a tissue inside the stem is to have a clean sample.. but it is possible to have it grow from the outer layer itself..
#9
Posted 24 February 2007 - 09:06 PM
Wow! i dident know pins could do that
#10
Posted 25 February 2007 - 10:54 AM
Ingenious! I've also cloned to LC from invitro PF jar pins. Worked great.
#11
Posted 25 February 2007 - 11:13 AM
Nice pics Hip. I like to cut little pins in half with the cut side down on the agar. Works great.