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Cloning Pins Directly to Agar


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#1 Hippie3

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Posted 24 February 2007 - 09:38 AM

pix stolen from some furry fucker
:pirate:

Attached Thumbnails

  • 926597212-Cloning_pins_2.jpg
  • 926597204-Cloning_pins_1.jpg


#2 fucgubarn

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Posted 24 February 2007 - 11:03 AM

the pix are nice :D

#3 nepenthes_ak

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Posted 24 February 2007 - 11:08 AM

:loveeyes:

Wow thats cool thanks for sharing hip! Is the one dried?

#4 fucgubarn

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Posted 24 February 2007 - 11:16 AM

i do not see dryed tissue here

#5 Hippie3

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Posted 24 February 2007 - 12:17 PM

fresh pins

#6 rikkyjsakki

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Posted 24 February 2007 - 02:14 PM

Hi Hip, what agar-nutrients do you typically use for plates like this? Is is potato-dextrose?

Thanks

#7 message2harry

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Posted 24 February 2007 - 06:50 PM

Doesn't the mycelium start growing from the stem butt? I see in those pics some of them were sliced open?

#8 Guest_vinz_*

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Posted 24 February 2007 - 08:04 PM

mycelium grows anywhere from a fresh mushroom.. the reason why people cut the inside to get a tissue inside the stem is to have a clean sample.. but it is possible to have it grow from the outer layer itself..

#9 kiddo

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Posted 24 February 2007 - 09:06 PM

Wow! i dident know pins could do that

#10 Sidestreet

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Posted 25 February 2007 - 10:54 AM

Ingenious! I've also cloned to LC from invitro PF jar pins. Worked great.

#11 SharkieJones

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Posted 25 February 2007 - 11:13 AM

Nice pics Hip. I like to cut little pins in half with the cut side down on the agar. Works great.




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