
In Praise Of Grain-2-Grain Transfers
#1
Posted 11 March 2007 - 05:43 PM
I took one jar of fully colonized rye and another of popcorn, cleaned them up and put them in the glovebox. I sprayed bleach water and wiped the entire box with alcohol soaked paper-towel.
Next I put the freshly PC'd and cooled jars of wheat, rye and popcorn. I used a sterile spoon and slid each lid off and added a heaping teaspoon of colonized grain. After I finished all the jars I rolled them around and shook them to redistribute the colonized pieces.
After 24 hours the mycelium had already taken over 3 grains in each direction, and after 48 , wow is it filling in fast. I did the transfers into pint jars about 5/8 to 2/3 full of grain and some 1/2 pint jars.
I have no problem being patient, but I must admit to the excitement of the running mycelium full steam ahead. I'll include pictures later.
#4
Posted 12 March 2007 - 08:25 AM
#5
Posted 12 March 2007 - 05:40 PM
G2G is a fast and simple way to expand your spawn. :thumbup:
#6
Posted 12 March 2007 - 08:43 PM
spores, maybe I'll try spreading some rye across a jar or two.
btw, nice job
#7
Posted 13 March 2007 - 11:51 PM
I took that straw (with all info I gleamed from here at the topia of course) and soaked it in a bucket with a cup of bleach for several hours. The manure I baked in the oven at 165 degrees or so for 1 3/4 hours. I added the straw and manure with some vermiculite into a pillowcase and then pasteurized at 165 ish for 1 hour plus. I used a candy thermometer to stay the course.After the pasteurization I hung, squeezed, drained the pillow case and allowed it to cool. This time I was careful to never keep anything in direct kitchen air. I always placeed a clear garbage bag over all jars, trays, pillowcases, buckets etc. I have had a problem with trich so i'm trying to eliminate contam vectors. Also I cleaned the work area and tubs well with bleach and alcohol and wore gloves...
The bags you see I made myself out of foodsaver vacuum seal bags. I just went with what I had on hand. I made some large and some small. The large one has 50/50 pasturized straw with goat poo and some some verm and the small one has 50/50 composted pasteurized deer poo from the yard mixed with straw and verm.
I crumbled a PF cake of B+ into the large home-made myco bag and used popcorn spawn for the smaller one. I can actually see a good bit of growth after 24 hours at 82 degrees inncubation. It makes me really happy to see the mycelium expanding and feasting down. I expect contams because i'm a newbie, but I really am happy how far i've gotten so far. I hope to fruit the bags invitro, possibly adding casing if it seems right... and if they make it that far.
The tubs were loaded with about 3ish inches of the pasturized goat poo / straw / verm mix when it was field capacity wetness and cooled. I added holes on the top with pollyfil filters for airflow. They are innoculating in a large plastic bin at 82 degrees F. One tub you see has 2 PF cakes of TAZ crumbled and mixed into the goat poo / straw / verm and the other has a single PF cake of Amazon crumbled and mixed into it.
One of the jars you see is a pint of popcorn spawn master that I stated about 4 days ago. The strain is Amazon. It seems to be doing fine and running through the popcorn decently. I like popcorn for grain to grain transfers , especially if you use a generous amount of colonized grains to innoculate. Waiting the week or so for syringe innoculations seems to be skirting trouble with contams. But like I said i'm still very new so what do I know, except what i've read here... The other jar in the third pic on the tub is a pint jar grain spawn master of wheat berries. I like the way the mycelium runs through the wheat kernals similar to rye. The strain you see in the wheat jar is South American. All the strains are from sponsers here at the topia, but you knew that...
The last shot is of another tub, this one qa bit deeper of pasturized goat poo 50/50 with straw plus a bit of verm. This tub I used 2 Taz PF cakes to innoculate. There was a lot of crumbled mycelium in there ! Probably 20 % cake 40% goat poo , 40% straw.
Thanks everyone for your informative posts. This site is amazing.:headbang:
cheers
P.S: any comments, critiques, tips graciously accepted
#8
Posted 14 March 2007 - 11:29 AM
Whats the proceedure if the Lord willing these bags/tubs colonize? Do they need casing? My plan was to case most of them with say cactus soil and leave some uncased. Later on can I open the colonized bags in the GB and sprinkle on the casing?
Any help greatly appreciated
Hyphae
#9
Posted 14 March 2007 - 08:00 PM
cheers
more later
#11
Guest_vinz_*
Posted 14 March 2007 - 10:26 PM
#12
Posted 14 March 2007 - 11:11 PM
Luckily when my pint jars of G2G started to condensate, the mycelium had already run 30% so I rolled them around and shook the jars which absorbed the water droplets on the jar.
#13
Posted 15 March 2007 - 12:29 PM
The bags are doing good for now. They were spawned with colozined popcorn.
#14
Posted 15 March 2007 - 01:03 PM
are you composting that
or aging or leaching or ?
to prepare it for use ?
#15
Posted 15 March 2007 - 01:16 PM
goat poo eh ?
are you composting that
or aging or leaching or ?
to prepare it for use ?
I have an outbuilding on my land that used to be a pen for goats. It has been sitting there composting for 2 possibly 3 years. It appears to be lots of goat manure, composted urinated straw and straw itself , which probaly has a bit of hay mixed in. I was happy to go clean out this building and then realize it was 6 inches deep in compost...
http://mycotopia.net...40&d=1173847526
#16
Posted 15 March 2007 - 01:28 PM
http://mycotopia.net...10&d=1173922853
pics below are tub filled with 50 / 50 goat poo /straw mixed with 2 cakes of TAZ PF. On top of that is pint sized popcorn grain master and jars of wheat. Last 2 shots show organic wheat that has been colonized with Amazon from a rye G2G transfer inside the GB. The last pic is of the same jar 48 hours after shaking it to redistribute the kernals. Its really bouncing back.
#17
Posted 16 March 2007 - 02:30 PM
#18
Posted 16 March 2007 - 05:39 PM
I love the way that the mycelium of all the strains looks like compared with the brown of the wheat berries. Bright white on clay brown is quite stunning. It makes me feel good to know that the mycelium is happy.
#19
Posted 16 March 2007 - 05:52 PM
#20
Posted 17 March 2007 - 01:18 AM
:pirate: