I thought i'd do an update on my grain 2 grain transfer progress. The whole impitus for doing the G2G in the first place beyond the fact that I have always wanted to wanted to preserve some strains that I had bought and had sat in syringes for more than 6 months.
As I stated above I did all my transfers in my glovebox. First I wiped the entire box & glass with alcohol soaked rag, then loaded with jars. The jars had tinfoil lids. I sprayed a light bleach water into the GB and waited. Then I donned the gloves and opened each jar , used a sterile spoon to scoop two teaspoons of fully colonized grain into each recipient jar and closed their lid. When I was done I marked each jar with date and strain.
Here's some more pictures of what happens after you shake 30 % colonized jars of popcorn and wheat and then wait 3 days. One is a picture of the large jar spiked with with a fully colonized grain cake & 50/50 goat poo / straw with a bit of verm .There is also a pic of how a syringe-injected jar looks after one week and a pic of a 4 day old tub opened for the purposes of the picture. The last pic is the homemade spawn bag I made out of foodsaver bags mxed with 50 /50 goat poo / straw & a PF cake of B+ mixed in with it.
thanks
H

In Praise Of Grain-2-Grain Transfers
Started By
hyphaenation
, Mar 11 2007 05:43 PM
22 replies to this topic
#21
Posted 18 March 2007 - 12:54 AM
#22
Posted 18 March 2007 - 02:45 PM
Is it possable to do G2G neverending. like A innoculates B and B does C so forth forever?I read you can only do it a few times then you need to restart from spore or agar.
#23
Posted 18 March 2007 - 04:05 PM
I know what you mean. I was concerned that after a few the spent kernals from your orginal G2G might go funky. I am currently using a regime like this:
1. Innoculate from syringe PC'd popcorn or rye
2. Wait until 30 % colonized
3. Shake,roll jar to redistribute kernals
4. Let it colonize 100 % then shake, roll, hit on palm
5. Let recover for 24 hours
6. Inside glovebox which has just been cleaned and sprayed carefully open each jar and using a sterile spoon put your desired amount of colonized grain into each recieving 1/2 pint jar of grain.
7. Repeat from step 2 on again
8. When the 1/2 pint jar is fully colonized i'm transferring that into pint jars with some colonized grain.
From there I plan to use the pint of fully colonized grain for projects. I'm ending up with plenty enough spawn material when several of the pint jars colonize, and then I just start over with step one.
Take into account that i'm a well read newbie and am really talking through my hemp hat. I'm just sharing what i've learned and am putting into practice.
1. Innoculate from syringe PC'd popcorn or rye
2. Wait until 30 % colonized
3. Shake,roll jar to redistribute kernals
4. Let it colonize 100 % then shake, roll, hit on palm
5. Let recover for 24 hours
6. Inside glovebox which has just been cleaned and sprayed carefully open each jar and using a sterile spoon put your desired amount of colonized grain into each recieving 1/2 pint jar of grain.
7. Repeat from step 2 on again
8. When the 1/2 pint jar is fully colonized i'm transferring that into pint jars with some colonized grain.
From there I plan to use the pint of fully colonized grain for projects. I'm ending up with plenty enough spawn material when several of the pint jars colonize, and then I just start over with step one.
Take into account that i'm a well read newbie and am really talking through my hemp hat. I'm just sharing what i've learned and am putting into practice.