Sterilite, perlite, mycobags grow log (FIRST TIMER)
Posted 18 March 2007 - 10:30 PM
The gf and I decided to take a sneak peak tonight to see what's happened (this is our first time) and were very happy to see some mycelium forming in each of the mycobags.
Getting the bags into pure darkness with the heatbomb sure did the trick, so thank you again for helping with that!
Until the next update!
Posted 22 March 2007 - 01:07 PM
One strain, P.E. shows absolutely NO signs of growth at all..
I think we'll give it another week in the warm dark place before and hope for the best :-(
..any ideas on how to give this a helping hand?
Posted 24 March 2007 - 02:30 AM
Posted 24 March 2007 - 06:47 AM
good at all. :(
Posted 24 March 2007 - 10:27 AM
Posted 24 March 2007 - 10:29 AM
Posted 24 March 2007 - 10:32 AM
Posted 24 March 2007 - 10:36 AM
Posted 24 March 2007 - 11:37 AM
The F+ not having colonized the outsides, it's just about 2 weeks since innoculation, when does anyone thing we should dunk? That seems to be the only that seems possible to get any goods out of..
Posted 24 March 2007 - 11:44 AM
just after a harvest in between flushes to get it ready for the next flush. I wouldn't dunk when you still have uncolonized sub, your leaving that open for contaminate spores.
Btw, the light you mentioned in your other thread wont hurt you at all. Some times when peeps grow invitro they never put them in the dark. I'll keep my fingers x'd for the jars you have left that have not shown signs of contamination...:)
Posted 24 March 2007 - 11:49 AM
thanks for the help :-)
Posted 24 March 2007 - 12:01 PM
Posted 24 March 2007 - 02:27 PM
how long has it been(in days) that you have notice no growth?
Posted 24 March 2007 - 02:30 PM
usually the mycelium will sprout where the spores were innoculated, the spread out into the substrate from there. with grain bags, you can "knead" them to spread the mycelium throughout the bag.
however, it looks like you have contamination in all the bags. i would start over with new bags and new spore syringes. make sure you clean your work area with alcohol, bleach, lysol, etc. Then wear rubber gloves or at least take a shower and wash your hands with alcohol. Then flame sterilize the needle before injecting the bag. Seal the hole immediately and resterilize the needle before injecting the next bag.
doing all this in a "glovebox" will prevent contams even better, but is not necessary.
Posted 24 March 2007 - 02:51 PM
Yes, the needle was sterilized. As was the point of puncture. I don't know how we could have possibly practiced any better sterilization techniques, we are n00bs, but we were incredibly aware of sterilization. The work area was thoroughly cleaned before hand, are and our hands were washed and gloved, we did not touch -anything- without gloves on, and even then we were very methodical not to do/move/touch anything that we didnt need to.
Where are you seeing signs of contamination in the other bags? We aren't seeing any.. at all (other than the one bag which has been tossed). Perhaps you could point out what your seeing.. or somethin?
Could it have been the substrate, maybe? It had been frozen when we recieved it.. could it have been the spore syringes from ralphster? (I'd try another place, but I don't where else sells) I want to try Mycrotopia's bags -- but I'd have to buy 10, which would cost me $150 in enough spores to innoculate all 10 bags
thanks for the reply...
Posted 24 March 2007 - 09:08 PM
SWIM used mych that came from a bacteria laden jar with success. He took the healthy looking mychg and did a light bleach dunk, then spawned to coir/verm, it fruited ok with no further sign of bacteria. Usually, we don't use contamed mych but if it's all you have sometimes it's worth a shoot, you wouldn't be the first to get fruits.:amazed:
Spend $30 at wally world and make a proper glovebox ! The rewards are worth it. You can clean a room a million times take a billion showers and you still won't be as sterile as a sealed box with half a can of lysol sprayed in it. And you can go 3 days without showering and still get nice clean results. DO NOT FLAME STERILIZE YOUR NEEDLE IN THE GLOVE BOX. do it outside and fill the needle cover with isopropyl alchol and insert it back after sterilizing.
btw order 10 bags for $45 and hillbillys march special $25 = $65 including shipping and enough spores left over to innoc 40 more bags. 1cc per bag is all oyu need.
Posted 25 March 2007 - 12:09 PM
I'm not interested in ordering 10 bags, which is the problem. I don't have the room for them (or the spores).
We'd grow more -- if we could get stuff to grow and if we had more private space (which we don't, we're renting)
Posted 25 March 2007 - 02:28 PM
Posted 25 March 2007 - 05:58 PM
at approx how many days should I think about giving up, then? I thought in 2 weeks they'd be ready for a dunk and fruit.
I only think that 1 is contaminated (and it's been tossed), but JM thinks all are contam -- but I'm not understanding why he thinks that..
thanks again :-)