P. Cyans In A Homemade Mycobag
Posted 24 March 2007 - 10:40 AM
The LC is my second attempt. First try was ruined by lack of air I believe and mabye carmelized sugars. This time I used a brand new distilled water bottle. I poured the contents into another clean bottle. To 1000 mils of distilled water I added 5 teaspoons of Karo and 2 teaspoon Light Malt Powder for a total of 7 teaspoons sugars to 1000 mils water. Next I shook up this mixture with the cap on and then poured it through a coffee filter in a funnel back into the original bottle. I put a pollyfill cap with a large hole in the bottle cap. I added 2 clean marbles.
I can't really shake the bottle with the pollyfill lid on so when I want to shake it I take it into the glovebox and replace the polly lid with the orginal cap, shake, then replace the polyfill lid.
I have the bag and the LC bottle on the floor where the tempsa are about 73 ish. I have read that P. Cyans needs cooler temps through inncubation. Does this sound right??? Anyone ??
Posted 24 March 2007 - 01:24 PM
more like 50ish degrees then 70.
you didnt need to pasturize the woodchips either, but i dont think it will hurt.
keep the pics coming, il be stoppin in to check em out.
Posted 24 March 2007 - 02:18 PM
Posted 24 March 2007 - 03:09 PM
Try keeping the lid switching to a minimum, imo. All the more chances to contam.
Posted 24 March 2007 - 03:27 PM
Posted 24 March 2007 - 03:28 PM
Posted 24 March 2007 - 03:33 PM
Just to go over that agin I used 5 tablespoons karo and 2 tablespoons light malt powder in 1000 mils of alder soak water. 7 tbs in 1000 is too many eh?? What would you say is ideal in 1000 mils water for woodlovers???
thanks in advance
Posted 24 March 2007 - 03:58 PM
You want about 4 tablespoons for 1000 ml. If it helps to remember, you can do a rule of thumb: 1 tblspn per cup of water. 1000 ml is close enough to 4 cups. You might be okay tho.
Posted 24 March 2007 - 05:54 PM
I hope that the blend I did catches on. If not I know better for next time. What do you think about karo mixed with light malt powder (filtered) ??? Come to think of it I used 5 tsp karo to 2 skimpy tsp malt powder. Still likely too much sugars...
Posted 25 March 2007 - 07:50 AM
Your mix sounds great. A varied sugar source. Should perform well.
Posted 25 March 2007 - 01:43 PM
I appreciate the comments. I did find the whole TSP vs TBL spoon ratio confusing but I think i've got it sorted now. The main diference in my LCV this time is that I have a large hole in the bottle cap stuffed with pollyfill. THis is allowing the LC to breathe way more than my last endevour which had 2 taped holes. The water bottle I used was clean and brand new.
I'm guessing that everything with P. CYans takes a bit longer than other species like cubies, so i'm trying not keep checking everyday for sign of life. One of these times i'm hoping to see the tell-tale signs of mycelium growth.
Cheesr & Thanks