
P. Cyans In A Homemade Mycobag
Started By
hyphaenation
, Mar 24 2007 10:40 AM
10 replies to this topic
#1
Posted 24 March 2007 - 10:40 AM
This is my first attempt at woodlovers. I soaked the alder chips for 24 hours drained and then pasturized in a pillowcase at 155 degrees F for 1 hour. Rinsed well and drained then stuffed into clean food-savers bag fitted with pollyfill filters.The tape in the bottom right corner is where I put tape over the water drain hole (to remove excess moisture)
The LC is my second attempt. First try was ruined by lack of air I believe and mabye carmelized sugars. This time I used a brand new distilled water bottle. I poured the contents into another clean bottle. To 1000 mils of distilled water I added 5 teaspoons of Karo and 2 teaspoon Light Malt Powder for a total of 7 teaspoons sugars to 1000 mils water. Next I shook up this mixture with the cap on and then poured it through a coffee filter in a funnel back into the original bottle. I put a pollyfill cap with a large hole in the bottle cap. I added 2 clean marbles.
I can't really shake the bottle with the pollyfill lid on so when I want to shake it I take it into the glovebox and replace the polly lid with the orginal cap, shake, then replace the polyfill lid.
I have the bag and the LC bottle on the floor where the tempsa are about 73 ish. I have read that P. Cyans needs cooler temps through inncubation. Does this sound right??? Anyone ??
The LC is my second attempt. First try was ruined by lack of air I believe and mabye carmelized sugars. This time I used a brand new distilled water bottle. I poured the contents into another clean bottle. To 1000 mils of distilled water I added 5 teaspoons of Karo and 2 teaspoon Light Malt Powder for a total of 7 teaspoons sugars to 1000 mils water. Next I shook up this mixture with the cap on and then poured it through a coffee filter in a funnel back into the original bottle. I put a pollyfill cap with a large hole in the bottle cap. I added 2 clean marbles.
I can't really shake the bottle with the pollyfill lid on so when I want to shake it I take it into the glovebox and replace the polly lid with the orginal cap, shake, then replace the polyfill lid.
I have the bag and the LC bottle on the floor where the tempsa are about 73 ish. I have read that P. Cyans needs cooler temps through inncubation. Does this sound right??? Anyone ??
#2
Posted 24 March 2007 - 01:24 PM
yes you will need much lower tempatures to inncubate or fruit cyans or azures.
more like 50ish degrees then 70.
you didnt need to pasturize the woodchips either, but i dont think it will hurt.
keep the pics coming, il be stoppin in to check em out.
more like 50ish degrees then 70.
you didnt need to pasturize the woodchips either, but i dont think it will hurt.
keep the pics coming, il be stoppin in to check em out.
#3
Posted 24 March 2007 - 02:18 PM
Colonizing at room temp is ideal, only the fruiting stage requires cooler conditions..
#4
Posted 24 March 2007 - 03:09 PM
I'm confused, sorry. What are you trying to do? Put the LC onto the past. woodchips? Maybe I misread? Anyhow, throw your past. chips in the freezer or something for now. Put that LC into some pf cakes or some hybrid wood/pf type cakes. Check out waylits woodlover tek. Its in the Vaults. When they are colonized, you spawn that to the chips.
Try keeping the lid switching to a minimum, imo. All the more chances to contam.
Try keeping the lid switching to a minimum, imo. All the more chances to contam.
#5
Posted 24 March 2007 - 03:27 PM
Bobcat I was saying that my first cyans LC went bad. Then I started a new batch with proper air-exchange via pollyfill in the bottle cap (decent sized hole in the cap). Injected the cyans into the bottle inside the glovebox.
#6
Posted 24 March 2007 - 03:28 PM
Way to much suger, IMO. Three teaspoons is plenty per 1000ml. H2O! You are more like preserving your spores than feeding.:rasta:
#7
Posted 24 March 2007 - 03:33 PM
Thanks rodeye , ive been trying to get the proportions of sugar to water straight. I know its suppose to be 4 - 6 % but opinions seem to vary. I was seeing one tablespoon per 100 mil of water for cubes. Waylitjim stated a number lower than that for woodlovers. Dang it now i'm out of Cyans spores. Oh well I was meaning to order some off the good captain anyways...
Just to go over that agin I used 5 tablespoons karo and 2 tablespoons light malt powder in 1000 mils of alder soak water. 7 tbs in 1000 is too many eh?? What would you say is ideal in 1000 mils water for woodlovers???
thanks in advance
H
Just to go over that agin I used 5 tablespoons karo and 2 tablespoons light malt powder in 1000 mils of alder soak water. 7 tbs in 1000 is too many eh?? What would you say is ideal in 1000 mils water for woodlovers???
thanks in advance
H
#8
Posted 24 March 2007 - 03:58 PM
Oh, I see now.... ;)
You want about 4 tablespoons for 1000 ml. If it helps to remember, you can do a rule of thumb: 1 tblspn per cup of water. 1000 ml is close enough to 4 cups. You might be okay tho.
You want about 4 tablespoons for 1000 ml. If it helps to remember, you can do a rule of thumb: 1 tblspn per cup of water. 1000 ml is close enough to 4 cups. You might be okay tho.
#9
Posted 24 March 2007 - 05:54 PM
Gracias !
I hope that the blend I did catches on. If not I know better for next time. What do you think about karo mixed with light malt powder (filtered) ??? Come to think of it I used 5 tsp karo to 2 skimpy tsp malt powder. Still likely too much sugars...
Again Thanks
H
I hope that the blend I did catches on. If not I know better for next time. What do you think about karo mixed with light malt powder (filtered) ??? Come to think of it I used 5 tsp karo to 2 skimpy tsp malt powder. Still likely too much sugars...
Again Thanks
H
#10
Posted 25 March 2007 - 07:50 AM
No, because you used TEASPOONS right? There are three teaspoons in a TABLESPOON. Dunno off the top of my head what the ml conversion would be... but basically you could have used up to 4 tablespoons but you only used 2 tblspns plus 1 tspn. So your on the light side. But you were adding this to alder soak water so you should be okay. In fact, maybe better off. I dunno. Better to be light on sugar than heavy!!!!! Some people prolly think a tablespoon to a cup of water is a bit heavy. But its done well for me.
Your mix sounds great. A varied sugar source. Should perform well.
Your mix sounds great. A varied sugar source. Should perform well.
#11
Posted 25 March 2007 - 01:43 PM
Thanks Bobcat.
I appreciate the comments. I did find the whole TSP vs TBL spoon ratio confusing but I think i've got it sorted now. The main diference in my LCV this time is that I have a large hole in the bottle cap stuffed with pollyfill. THis is allowing the LC to breathe way more than my last endevour which had 2 taped holes. The water bottle I used was clean and brand new.
I'm guessing that everything with P. CYans takes a bit longer than other species like cubies, so i'm trying not keep checking everyday for sign of life. One of these times i'm hoping to see the tell-tale signs of mycelium growth.
Cheesr & Thanks
H
I appreciate the comments. I did find the whole TSP vs TBL spoon ratio confusing but I think i've got it sorted now. The main diference in my LCV this time is that I have a large hole in the bottle cap stuffed with pollyfill. THis is allowing the LC to breathe way more than my last endevour which had 2 taped holes. The water bottle I used was clean and brand new.
I'm guessing that everything with P. CYans takes a bit longer than other species like cubies, so i'm trying not keep checking everyday for sign of life. One of these times i'm hoping to see the tell-tale signs of mycelium growth.
Cheesr & Thanks
H