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Another try at cloning Pans


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#1 spacecake

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Posted 30 March 2007 - 02:50 PM

Hi Mycotopian friends...!

I still have failed to succesfully clone a Pan shroom so far !
Now I'm going to give it another try with a different strain..Pan Cambodian !

The Florida lg's didn't do well as clones,so that's why I'm giving Cambo's a shot..altho Florida's are still my favorites as multispore...

I have taken the inside of a stem,and did put it in a jar full of broken glas (picture 1).
Shaked it for 5 minutes very well,and sucked it up in a syringe..
I used 5 ml for one jar.
Injected this in two jars..,one jar is brf and the other Corn.

The brf jar was very quick,while the corn is only half way colonized !
I notice that the corn here is getting old and not so good anymore !

So far I made two casing trays,with my usual 'recipy',but added only extra verm on one tray.
They are fully re-colonized and in the terrarium at 25 oC.

Hopefully this will be a succes,because my last tray gave only 10 grams (wet) of shrooms :thumbdown: !
Wish me luck !

Attached Thumbnails

  • Casing readyint.jpg
  • DSCN3480int.jpg


#2 golly

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Posted 30 March 2007 - 04:18 PM

Tray looks great Space...I'm interested to know more about your clone injection method...Sounds really simple...

#3 Godflesh

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Posted 30 March 2007 - 04:22 PM

Very nice lookin tray Space!:bow:

#4 spacecake

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Posted 30 March 2007 - 04:31 PM

Tray looks great Space...I'm interested to know more about your clone injection method...Sounds really simple...


Thanks..guys !

Well it's the same way as normal ,only now I suck up the 'clone solution' out of the jar..into the syring and inject the brf jars the same way as the pf tek...

So..no special way here,same as with cubes..whole stem in peroxide didn't work for me,so now I took a big stem.. and peeled the outside off,and droped the inside in the jar with broken glas and sterilized water.
No peroxide used in this experiment !

#5 Hippie3

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Posted 30 March 2007 - 06:11 PM

so those trays ARE clones right ?
what went wrong with the FL ?

#6 spacecake

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Posted 31 March 2007 - 03:40 AM

so those trays ARE clones right ?
what went wrong with the FL ?


Yep,...!

Well the Lg's gave me pretty good flushes multispore,but when I tried to clone them,they gave only a few shrooms !
Also,the mycelium growth was very heavy,and got overlayed....

#7 CoyoteMesc

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Posted 04 April 2007 - 11:55 AM

Any updates on the LC?

#8 spacecake

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Posted 04 April 2007 - 12:21 PM

Well, I'm getting really frustrated now,..and shouting at my trays... ''What the hell are you hiding for ! " lol...

So..still no sign of any pin formations !
Next time I will deffenitly give my poo a bleach wash,..see if that will help !

Because I'm getting bored,I have made a Australian casing (cubes) for fun, and gues what...easy stuff...I had pins in three days...grrrrr NOT FAIR !
I want Pans not Cubes....!

#9 Guest_lost_onabbey_rd_*

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Posted 04 April 2007 - 01:38 PM

you shouldn't yell at them space
instead try sweet talking them
try to bribe them with promises of extra FAE or a good misting
good luck with them though bud.
lost

#10 Hippie3

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Posted 04 April 2007 - 07:12 PM

patience is a virtue.
:bow:

#11 spacecake

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Posted 05 April 2007 - 12:15 PM

Wow,somebody prayed for me...? lol

When I got home from work,I saw 10 pins..divided over three trays ! :) (two of them the cambo clones)
I don't know what strain I've got in the third tray,..Hawaii ? ,..maybe someone can identify it ,when they more matured...

Hopefully it will be a good flush this time,Because I don't wanna bleach the poo ,if I don't have too !

Some pics...not spectaculair,but it's all I have for now... ;)

http://mycotopia.net...56&d=1175793261

Attached Thumbnails

  • 1st Pinint.jpg
  • 1 st Pinsint.jpg


#12 golly

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Posted 05 April 2007 - 01:20 PM

Excellent...Sing em a song and you'll be seeing purple bunnies by Sunday ,i promise...heh...

#13 spacecake

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Posted 05 April 2007 - 01:32 PM

:D.... :lol:




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