
Rubbing alcohol stalling growth
#1
Guest_infected_*
Posted 14 February 2005 - 12:48 AM
#2
Posted 14 February 2005 - 03:46 AM
(Message edited by looper on February 14, 2005)
#3
Guest_rodger_*
Posted 14 February 2005 - 06:33 AM
#4
Guest_ªb·sinth_*
Posted 14 February 2005 - 07:46 AM
Alcohol won't sterilize anything<!-/quote-!><hr size=0></blockquote>
what about bleach?
#5
Guest_hippie3_*
Posted 14 February 2005 - 07:57 AM
is that a brief exposure to alky
is insufficient.
so a quick dip isn't helping.
prolonged exposure will kill,
as will a brief flame, of course.
one generlly needs neither
as long as the needle never contacts
unsterile surfaces.
#6
Guest_ªb·sinth_*
Posted 14 February 2005 - 08:05 AM
#7
Guest_vrooota!_*
Posted 14 February 2005 - 08:14 AM
#8
Guest_rodger_*
Posted 14 February 2005 - 10:44 AM
i tend to keep away from flames, i'm always afraid of flammable gasses in the glovebox<!-/quote-!><hr size=0></blockquote>
There is no need for flammable gasses in a glovebox. Soap and water is all that is required.
#9
Guest_infected_*
Posted 14 February 2005 - 04:34 PM
#10
Guest_the_*
Posted 14 February 2005 - 05:15 PM
#11
Posted 14 February 2005 - 05:29 PM
What? Don't toss those jars out, they might still be good<!-/quote-!><hr size=0></blockquote>
Right don't toss them yet.
I use alcohol soaked towel to wipe my syringe as well, and only flame sterilize between inserting my needle in to the jar of mycelium water.
I do not wipe for every jar though.
For cakes I inoculate 2 half pint jars for every 10 mL syringe. I wipe after injection of mycelium and set it aside giving just enough time to take the foil off and loosen both jar lids.
I then very steadily, inoc. 5 points for each jar(i.e. the corners and middle). I then move quickly to the next jar and repeat.
This is just the way I have grown comfortable with and have great success. Every one has thier own way of cutivation.
(Message edited by Fender on February 14, 2005)
#12
Posted 15 February 2005 - 09:11 AM
For cakes I inoculate 2 half pint jars for every 10 mL syringe.<!-/quote-!><hr size=0></blockquote>
Isn't 1cc the same as 1ml? If it is, your using 5cc's inoculant per Jar?
#13
Posted 15 February 2005 - 02:40 PM
#14
Posted 15 February 2005 - 02:50 PM
#15
Guest_rodger_*
Posted 15 February 2005 - 02:55 PM
I fail to see any reason why one would wish to spray a surface disinfectant inside a glovebox. Do you intend to dump the contents of your jars out onto the floor of the glovebox?
#16
Posted 15 February 2005 - 08:55 PM
yes they are the same.
I don't use spore syringes anymore, I use mycelium water, as I make a small mouth jar 3/4 the way full w. the stuff.
So I do overkill, but i have so much to throw around.
And after the mycel. recovers, my jars are almost 1/2 way colonized.
(Message edited by Fender on February 16, 2005)
#17
Guest_a.k.a._*
Posted 15 February 2005 - 08:59 PM
#18
Guest_rodger_*
Posted 15 February 2005 - 09:06 PM
#19
Guest_a.k.a._*
Posted 15 February 2005 - 09:27 PM
Could you do this in a bathroom then, if it was your only draft free room?
#20
Guest_rodger_*
Posted 15 February 2005 - 10:13 PM