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Liquid culture in a syringe

culture liquid syringe

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#1 waylitjim

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Posted 19 March 2005 - 10:50 PM

Recenly had an idea for creating liquid culture without using jars...
i haven't tried it yet, but seems like it would work.

Syringe Liquid Culture Tek:

1. Boil 12 ounces of water on the stove

2. Add 5 teaspoons of Dextrose/Karo to boiling water and stir in untill dissolved

3. Suck up 12 ml boiling sugar water into each syringe

4. Wrap syringes in saran wrap and let them cool to room temp.

5. Shoot 4 syringes (48 ml) into a ziplock bag containing a sterile print on foil

6. Rub between thumb and forefinger to disperse spores with the sugar water.

7. Suck the spore water back up into the 4 syringes by injecting thru the bag

8. Wrap the syringes back up in saran wrap and leave at room temp

9. Wait 1-2 weeks and you should see mycelium growth in each of the syringes

10. Inject PF or grain jars as you normally would

Jars innoculated in this manner should colonize much faster
since the spores hatch in the syringe before being injected into the jar.

:)
~Strangeland

#2 insight

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Posted 19 March 2005 - 10:53 PM

Its make sense. This way youre not having to draw out of a jar each time.

#3 destroy_erase_improve

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Posted 19 March 2005 - 10:56 PM

i like the idea, but doesnt the 1-2 week wait for the syringes to turn "hot" offset the cut in colonization time from the jars?

if you innoculate jars with sporewater it takes about 14 days for full colonization. give or take.
if you wait two weeks and then noc up with the hot syringe, it just about equals out.

or am i missing something?

#4 waylitjim

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Posted 19 March 2005 - 11:39 PM

One advantage is that after 2 weeks you can do a S2S transfer (syringe to syringe) :)
By promotimg mycelium growth in the syringe u can multiply the number of syringes u have with the following tek:

1. Shoot one 12 ml myc syringe into a sterile baggie

2. Shoot 36 ml of sterile water into the baggie

3. Mix together and suck the water back into 4 syringes,
creating 4 viable syringes from one.

-Strange

#5 destroy_erase_improve

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Posted 19 March 2005 - 11:44 PM

ah haaaa!
not bad, not bad at all!

i see where your going with this
one syringe of the live myc will have PLENTY of hot happiness in it
if done properly
way more than needed for one syringe really
so a person would be able to dilute it
into four syringes
all filled with hot myc

good thinking

#6 waylitjim

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Posted 20 March 2005 - 12:01 AM

ah haaaa!
not bad, not bad at all!

i see where your going with this
one syringe of the live myc will have PLENTY of hot happiness in it
if done properly
way more than needed for one syringe really
so a person would be able to dilute it
into four syringes
all filled with hot myc

good thinking


Thanks :)
I'll test it and see if a good boil is just as effective as a 15 minute PC.
Most liquid culture teks call for pressure cooking the dextrose/karo water.
Boiling spore water to make syringes works great, so why not start with karo water!

#7 imok

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Posted 20 March 2005 - 12:45 AM

Most liquid culture teks call for pressure cooking the dextrose/karo water.

You haven't read that in the teks that are posted here.
Karo/Honey/Dextrose(NY) solutions generally only require boiling or microwaving.
They are fairly sterile already.
Quantity wise, 4 syringes/print is kinda low imo.
With a simple glove box and liquid culture medium you could get that out of a 1/4 (or less) print, not a whole print.

#8 waylitjim

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Posted 20 March 2005 - 01:25 AM

You haven't read that in the teks that are posted here.


Quoting one of Nans L.C Teks in the mycotopia archives...

"The Karo jar is made up and Sterilized with a solid dome lid, inverted so that the seal side is up (we don't want to can it) and the band screwed on loosely. Foil Cover of course. PC time is 20 minutes. "

http://mycotopia.net...html?1037043810

here's another example

http://mycotopia.net...html?1069714050

#9 nicholas

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Posted 20 March 2005 - 01:33 AM

You can get away with microwaving your sugarwater solution, but I'd just as soon use a PC as microwaves are filthy.

#10 waylitjim

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Posted 20 March 2005 - 01:37 AM

You can get away with microwaving your sugarwater solution, but I'd just as soon use a PC as microwaves are filthy.


I guess you could always clean it LoL...
If I had one.... I'd only use it for liquid culture...they make food taste bad.

#11 imok

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Posted 20 March 2005 - 02:11 AM

Sorry, guess I should have said recent posts/teks in the archives :)
Check out Hips poor mans syringe tek using a microwave.
We just recently discussed it, Poor Man's Syringe Tek: REALLY?
Recent discussion:
http://mycotopia.net...&highlight=poor
Archives:
http://mycotopia.net...html?1077458711

#12 perrch01

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Posted 20 March 2005 - 08:23 AM

Have you considered stretching the initial syringe amount? Perhaps instead up putting in 48ml and splitting that between four syringes you could take like 32 syringes almost full of you dextrose water and suck up 1.5cc from the spore print baggie? Might take a little longer but would allow you to spread the wealth easier, or not

#13 Quartz

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Posted 20 March 2005 - 08:37 AM

Ive used the microwave for my liquid cultures like it was a religion.
I take a half pint jar make up my solution cover the jar with cerain wrap and a rubber band.
Put in the nuckerwave and turn it on . I run the jar just untill it starts to boil then stop the nuckerwave let it cool for a few minutes and repeat 2 more times . Transfer the jar into my glovebox where there is a foil wraped sterilized lid waiting to to be put on. Transfer some tisue, hot solution, or spores into the jar and incubate for a week.
Good to go shoot em full.

#14 perrch01

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Posted 20 March 2005 - 08:39 AM

THe microwave tek is the only one I use as well. Do you use any type of a filter that allows air exchange?

#15 Guest_Peter Cottontail_*

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Posted 20 March 2005 - 12:05 PM

I would think if you allowed the myc to grow in the syringe, it would be very hard to get back out. Perhaps you could stick a bb or something in the syringe for later shaking.

#16 nomoreusmc

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Posted 20 March 2005 - 12:37 PM

peice of broken glass would probibly work better than a bb

#17 smegma

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Posted 20 March 2005 - 12:46 PM

One problem I see... no AIR.

Your Myc will suffocate rather quickly I would think.

#18 waylitjim

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Posted 20 March 2005 - 01:12 PM

One problem I see... no AIR.
Your Myc will suffocate rather quickly I would think.


There is enough air in the syringe for mycelium to grow out, check the Poor Mans Syringe Tek.
Thanks for that link Imok. :)

After reading that tek, it makes more sense to start with an isolated strain in a colonized jar,
this way your taking spores out of the picture and using a tried and true isolate to start new jars.

#19 smegma

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Posted 20 March 2005 - 01:15 PM

There is enough air in the syringe for mycelium to grow out, check the Poor Mans Syringe Tek.
Thanks for that link Imok. :)

After reading that tek, it makes more sense to start with an isolated strain in a colonized jar,
this way your taking spores out of the picture and using a tried and true isolate to start new jars.


When my FOAF makes liquid cultures, ones on a stir plate grow 2x as fast and 5x as strong as the ons that are not. Yes, myc may grow in it, but it would be a lot faster and stronger if it was air-ated :).

This is my experience. Half pint jars filled half way will probably treat you better.

#20 Guest_novak_*

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Posted 04 May 2005 - 08:40 PM

i don't remember where i read this but i read once that it was possible to inject water from a sterial syringe into a fully colonized PF cake in a jar. one must shake the water around and then suck the water back out using an innoc hole. the water would have live myc in it from being injected into the cake and since most fully colonized cakes in the sealed jars are contam free, with a little care and clean syringe, one could easily make many syringes from a single cake that were clean and easy. is this bunk?





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