Straw Bags, strain unknown.
Posted 17 May 2007 - 12:53 AM
back a year or so ago a guy had good success with his first and only straw bag attempts, which involved cutting straw 3" and shorter, loading ziploc gallon bags 1/3rd full, and filling to the top with hot water, a dash (unmeasured, from jug) of bleach, and some gardening lime (he’s heard this is the wrong kind.)
anyway, a guy believes he spawned those straw bags with BHT or GT.. and printed a large 8" + monster.. one of like three that actually came off the bag on its second flush (didnt dunk, first round a guy let it go invitro). ofcourse, a guy didn't label the print, so he can only conclude it's BHT or GT.
Now, with the way-back background complete, on to the more recent.
per some ballsy LC technique a guy made a honey jar in a half pint jar (used a tall filled 3/4ths full), did two of those actually. did open air inoc scraping from print to jar, a dash of oust in the air, working about 6" away from an open window with fan sucking out (sucking all the nasty in the room out past his work area lol).. and also about a foot away from his cat's food and water bowl!
success, this small batch of LCs has now created more LC than he needs for a while ;)
lc used on over 20 half pints. after doing some much needed testing, there will no longer be any lids used in any operations! brf cakes, verm barrier, then foil and a rubberband. very pleasing, allows for quick whipe of entire foil top with alcohol, flame of the needle, and an instant 8ish inoc sites with an excess of LC :)
so the jars are done, now we move in to the present:
no lime this time, he figured he used the wrong type anyway last time and it worked.. again used the pellet outdoor garden lime he guesses it was called. cheap 5g wallmart trashcan, ziplocs filled ..packed.. and knife slit, 3 of them, thrown into bottom. hot hot hot tap water, then a half cup of bleach say in this 3 gal of water.. and submersed for 24 hrs.
retrieved bags, squeezed hard (still looked a little damp), then dumped it into new bags (made 4 out of 3 initally). cakes were dumped into a mixing bag verm barrier and all to compensate for wet straw. broke up as tiny as possible in the mixing bag and them dumped into whatever bags.
top 2 bags here split 4 hpints's, bottom two split 3.
this is like 36 hours he says :headbang:
jar same age as anything else. chinese resturant soup to gocontaner
Posted 18 May 2007 - 05:01 AM
Posted 18 May 2007 - 11:36 AM
grains would be nice but 10qt PC doesnt seem like itd house many jars. curious about the soap addition to sterilizing grains, could possibly allow for microwave usage with success ;D
Posted 18 May 2007 - 02:53 PM
Posted 01 June 2007 - 10:19 AM
a time line..spawned 5.18 with 1.5 - 2.0 hpints of mystery strain..
fully solid bricks of love by the 25th.
pins on the 29th. i think pins would have been here sooner had i exposed to light during the colonization of the straw (since my intentions were to fruit in bag/ still are).
the little cup thing is doing fine, it has probably 200 tiny pins on it but it's incredibly dehydrated as is so i dont have high hopes.
the bags as it stands.. 3 surviving, all have pins, one has like 4 pins and one sucker the size of my thumb already.. one has like 3 or 4 tiny pins way down at the bottom of the bag (not ideal to be there, too cramped).. and then the third bag is the role model, its got like 30 pins all about an inch long :amazed:
trying to leave em still in their drawer for now til picking, so i dont think ill have any pics worthy of uploading til then.
filter patches im real proud of.. i cut out a section of duct tape, say a strip 2" long.. and the razor'd out a circle in the center of the tape. folded a nylon fish tank filter sock over twice and stuck on the sticky part of the tape, with more stickyness still surrounding it..and slapped that over a dime sized hole i put in each bag.
took 2 minutes :D working great, doubled over tihgt knit nylon seems to be just right.
Posted 01 June 2007 - 06:42 PM
Posted 01 June 2007 - 08:13 PM
did you make 3" slits in the bags or just the filter patch?
Posted 02 June 2007 - 01:17 PM
i noticed that when there was a slow area of uncolonized straw, a few pin pricks right into it with a (nonsterilized) pin made things finish up.. definately beneficial.
once i put them into fruiting i did the diy filter patch. pics in a bit.
Posted 02 June 2007 - 01:36 PM
Posted 02 June 2007 - 04:10 PM
The reason behind poking holes in the top part only, and not all over the bag is because during the spawn run, while its colonizing, it doesn't need to have a lot of open air.
It can also cause the straw to dry out some before it finishes colonizing all the way through. Its also possible to introduce contams to the uncolonized portions of straw with too many holes.
I'm glad none of that occured when you did it, and that you observed better colonization times.
Keep sharing the pics, so we can see how they finish up invitro :)
Posted 04 June 2007 - 10:34 AM
last nights pics attached, havent done much since surprisingly.. probably gonna be good to go when i get home from work :)
these are either BHT or GT... guesses?
Posted 04 June 2007 - 10:44 AM
Posted 04 June 2007 - 08:46 PM
Those are beauties!
I really like the filter patch you fabricated into the bag! These did well invitro style like that.
Posted 04 June 2007 - 08:56 PM
Nice straw bags you got there :D
Posted 04 June 2007 - 11:46 PM
bag with many pins along the top is going slow but a rapid increase in size is expected soon :headbang:
cap taken from large mature cluster to print :)
third bag not pictured has 1/10th the amt of pins as the other two. oddly enoug h it's the smaller of the two spawned with 1.5 hpint cakes..
a recap of this procedure.. bleach + warm water + straw + as little as 10 minutes submerged (worked for first runs of these over a year ago).. drain and ring out, fill bags, let cool a min, spawn, incubate, cut filter patch..
though cakes are still being used due to small pc and cold feet about straight grain, if it proves to throw out more than the average cake then it's a-ok since the straw ordeal is..not so much an ordeal at all.
Look still like GT?
Posted 05 June 2007 - 10:22 AM
took 4 prints and one clone, hope it works ;D
Posted 05 June 2007 - 12:22 PM
Posted 05 June 2007 - 12:26 PM
some hardware stores too.