Help for newbie please
Posted 23 May 2007 - 02:52 PM
This is where I'm at.I've got spore prints,10 syringes,4 bonne mamam jars,glovebox,lysol (dettol),foil,oust spray and a pressure cooker.I reckon I will be able to make up the syringes without messing it up.
I need to get some vermiculite.I intend to put my substrate into the jars with a layer of vermiculite on top,pc,cool,and inoculate.
Can I use ordinary pop corn as a substrate or should I get organic brown rice?If I cant get vermiculite can I use perlite?Do I need to mix vermiculite with the brown rice/popping corn?
Thanks to mycotopia,I've got my head around the sterile procedure.But everything else is still alien to me.Your help is appreciated.:)
Posted 23 May 2007 - 04:21 PM
Posted 23 May 2007 - 04:50 PM
Posted 23 May 2007 - 05:44 PM
The spores are brasil and australian,a gift from spacecake.I've had failures in the past with bought syringes.I hear you joystick,I dont want to mess up again.
I'm going to use organic brown rice,ground in a food processor,mixed with vermiculite and water,in the proportions given by Nabby.Then dry vermiculite on top.
I'll post some pics in a few days.:)
Posted 25 May 2007 - 03:37 PM
How long do I need to pc them for?
Posted 25 May 2007 - 03:54 PM
Posted 25 May 2007 - 04:39 PM
Posted 26 May 2007 - 06:07 AM
I only managed to make up one syringe.I managed to inoculate 3 jars.Most of the syringe went into the first jar.
If these jars contam then I'm going to buy a glovebox.I'm not feeling confident at this stage.:(
Posted 26 May 2007 - 09:23 AM
Posted 26 May 2007 - 10:37 AM
I wish I'd just dropped the foil into the jar,put the lid on and swirled it around.Or injected into the bag and sucked it back up.I was trying to be as quick as possible to avoid contams.
The inoculation was easy enough.I just dont know if any spores made it into the syringe.I'm such an idiot!:lol:
Posted 26 May 2007 - 11:09 AM
really helps get those spores off
Posted 26 May 2007 - 12:06 PM
Just clean your knife with alcohol,and scrape of the spores...
witch strain are trying ?
Posted 26 May 2007 - 12:27 PM
I'm going to make a glovebox with proper gloves that clamp on.
Posted 29 May 2007 - 11:42 AM
Posted 29 May 2007 - 12:16 PM
What's the incubation temp ?
Posted 29 May 2007 - 01:38 PM
Posted 01 June 2007 - 05:17 AM