Dank-Ass Liquid Culture-Tek!
Posted 12 October 2007 - 03:20 PM
Posted 12 October 2007 - 05:10 PM
What a wonderful and well written tek
Hello LC here I come
Thanks for the tips:bow:
Posted 12 October 2007 - 05:41 PM
great write up btw...thanks a ton:headbang::headbang:
Posted 12 October 2007 - 09:43 PM
Posted 12 October 2007 - 10:10 PM
how many cc's does it take to then knock up a myco bag or jar effectively
I use LC liberally, as in I would use 60-120cc in a single mycobag with a h-poo based sub between 5-10lbs. You should get good colonization speed from that :thumbup:
Posted 30 November 2007 - 02:52 PM
Posted 13 December 2007 - 10:34 PM
Ive got a jar of LC (hillbilly) growing now, and theres prolly a couple teaspoons worht of growth in there after a few days... goin pretty quick from what I understand........ now..
when it is ready to put into syringes and use...
does the mycellium suck right up through the needle itself?
or does it need to be sucked up without the needle attatched? or how's that work?
appreciate it, Bless
Posted 14 December 2007 - 08:39 AM
I get some 90% Iso Alky and fill an empty syringe, then I fill the needle tip cover with the alky, then I dump the alky filling the syringe in a shotglass, wipe the injection point with alky, dip the tip of the syringe in the needle cover and suck out the goodness. then Innoculate with a wipe and a dip before, I then dip the needle in the needle cover wipe the injection piont with alky and refil with LC. When finished I simply suck the alky back out of the shotglass and put the tip cover on with the alky in it and I am always ready to knock up some stuff.:rasta:
Posted 14 December 2007 - 03:59 PM
Why 16 gauge?
It's a bigger needle hole than the standard 18 gauge you get when you order a spore syringe. The Mycelium in your LC will (hopefully) be so thick that you'll have a much easier time sucking it through the needle and into the syringe if you use a 16 gauge needle that is 1.5" in length.
Obviously, you can re-use your old syringes, needles, needle gaurds, and twist caps. Just wrap each one (syringe/plunger, needle & cap) individually in foil and throw them in the PC for 20 or 30 min at 15 psi. When the PC is cool, take them out (but don't unwrap them from the foil) and put them in a fresh zip-lock bag until ready to use.
When ready to use, put the zip-lock bags containing your foil wraped syringes, needles, and caps into the glovebox. Then assemble each one inside the glovebox as you go. If done this way, they should keep sterile. Once assembled I usually will flame the needle till glowing red and then wipe the needle with a paper towel soaked with 91% iso just to be sure that the needle is sterile. Works like a charm.
Posted 16 December 2007 - 03:00 AM
Posted 16 December 2007 - 10:31 AM
how long do you pc it for or did i miss that part?
In the tek above it calls for 30 min in the PC at 15 psi.
Posted 18 December 2007 - 01:48 PM
Posted 18 December 2007 - 02:03 PM
there is noticeable growth in less than 48 hours, but then again my syringe had mycelia in it already so that helped :) oh, and will rubbing alcohol kill a liquid culture. i made an airport syringe and i soaked the polyfil in alkie to clean it. i thought it was dry but when i put it in it sucked a few cc's of 90% alkie in there lol. good thing i made two of em lol.
i dunno if the alkie will kill it. let us know.
i forgot to mention that my "7-10 days" results was from an existing LC to LC if that makes sense.
when doing a multispore to LC it took 3 weeks (on one occasion). i guess it depends.
once you get a good healthy LC from a multispore, then shoot your next LC with existing LC and it grows fast.