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Ultrasonification

ultrasonification

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#1 smegma

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Posted 23 March 2005 - 01:00 PM

This Ultrasonic cleaner was bought at Radio Shack for $30. Let's see what she does.

Picture 1: Dry Print in Half Pint
Picture 2: 150ml Sterile Distilled Water Added, SHAKEN HARD, nothing happened.
Picture 3: Ultrasonification (Ran for like 12 cycles... 1hr maybe)
Picture 4: Same jar after ultrasonification. The white is crud on outside.
Picture 5: NICE syringes. Yellow ones are for 'kitchen' (Wallyworld) and can be PC'd.
Picture 6: NICE glovebox. :D :D :D :D :D
Picture 7: You'll clean this cat box ANYTIME. (Criminis of COURSE!)

Kick some ass.

Can anyone tell my FOAF how to take better pictures?

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#2 troutlips

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Posted 23 March 2005 - 01:40 PM

Very interesting smegma, those syringes look very purple.
Could you give us more details about the ultrasonic device?
Cute kitty BTW.;)

#3 smegma

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Posted 23 March 2005 - 02:08 PM

Very interesting smegma, those syringes look very purple.
Could you give us more details about the ultrasonic device?
Cute kitty BTW.;)


My FOAF said 1qt. WBS was 50% colonized in 4-ish days when innoculated with 4cc of the purple goodness then shaken well. No incubation, kept 70f.

He would send me pics of said jars, but since the have poop in them and are a touch wet, people will be all "CONTAMS" and I do not want to confuse people.
http://www.radioshac...duct_id=63-1163

The cat is 18 yrs. old :eek: and VERY good luck.

#4 kikashi

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Posted 23 March 2005 - 02:39 PM

nice job smegma. How do you keep the glove box sterile. I mean the two holes in the side. what is your glove set up?

#5 smegma

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Posted 23 March 2005 - 02:56 PM

nice job smegma. How do you keep the glove box sterile. I mean the two holes in the side. what is your glove set up?


It is not sterile but not dirty.
I just spray the hell out of the inside of it with bleach, put on latex gloves and go at it.
I move VERY SLOWLY and don't stir up air.
The holes are where the arms go. :p
I also put things in through the holes.
I would not recommend this procedure to anyone.
The only thing I have ever needed it for is cloning, opening jars for printing and syringe making.
I do the rest open air.

How you work in the box helps too. For example, anything that touches the box I consider SOILED. so I never let a suringe, glove ANYTHING touch the box.

#6 kikashi

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Posted 23 March 2005 - 03:21 PM

The holes are where the arms go. :p

LOL.

Thx smegma, you are doing what I will be looking to do in the near future. Cap is printing as we post. My question was directed at the mechanics of using the box. Thx for the info.

#7 destroy_erase_improve

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Posted 23 March 2005 - 10:11 PM

crap those are some dark homemade syringes
very nice!
just goes to show you that anyone can afford to sonicate their syringes now
less than 30 bucks for your own personal shaker

btw, cute cat
i love felines

#8 smegma

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Posted 24 March 2005 - 09:02 AM

OK PROBLEM.

I did not use Jet Dry or Lube and within 24 hrs. all the spores settled and stuck to the side of the syringes.

I can "re-ultrasonificate" them so no worries, but I cannot find the formula on here to keep them in suspension.

First person to post it here will get one of those 40CC purple MFers. It is TEX and the Print/syringe is clean.

#9 kikashi

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Posted 24 March 2005 - 09:47 AM

You're putting that in the spore solution? I'm following this thread with some interest. the pic is from my most prolific EQ cake to date. and now that I know where the arms go...:o

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#10 smegma

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Posted 24 March 2005 - 09:54 AM

You're putting that in the spore solution? I'm following this thread with some interest. the pic is from my most prolific EQ cake to date. and now that I know where the arms go...:o


Yes in the solution to keep gravity from pulling the spores to the walls of the syringes. It keeps them suspended in the water.

That looks NICE!

I was kidding about the arms. :o

#11 Hippie3

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Posted 24 March 2005 - 09:56 AM

anyone can afford to sonicate their syringes now


yep.
and those are pretty concentrated,
they could be diluted and streched much farther,
a light lavendar color is way more than enough,
no need for purple/black syringes.

#12 kikashi

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Posted 24 March 2005 - 09:56 AM

thx, to you to Hippie3

#13 Hippie3

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Posted 24 March 2005 - 09:58 AM

cannot find the formula on here to keep them in suspension.


just be aware that adding such additives can trigger premie germination.
not for use in long term storage.

#14 smegma

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Posted 24 March 2005 - 10:01 AM

no need for purple/black syringes.


I agree that there is a point of diminishing returns.

Using fewer CC per jar has helped keep moisture levels correct.

Probably could have made 3x more but had no use for that many.

#15 Hippie3

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Posted 24 March 2005 - 10:01 AM

OK PROBLEM.

I did not use Jet Dry or Lube and within 24 hrs. all the spores settled and stuck to the side of the syringes.

I can "re-ultrasonificate" them so no worries, but I cannot find the formula on here to keep them in suspension.

First person to post it here will get one of those 40CC purple MFers. It is TEX and the Print/syringe is clean.

OK, found the JetDry part 1 drop to 20 CC.

How much lube?




Formulation of 6 drops JetDry & 4 drops AstroGlide per 1000ml distilled water

#16 smegma

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Posted 24 March 2005 - 10:02 AM

just be aware that adding such additives can trigger premie germination.
not for use in long term storage.


Hip, how much lube... I have searched, but cannot find it.

NM. :D Thanks hip!

If you want to give out 2 X 40cc Tex syringes I will send them out.

#17 Hippie3

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Posted 24 March 2005 - 10:23 AM

just post a contest in that forum
and give them away
in our behalf, plz.
thx

#18 Hippie3

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Posted 24 March 2005 - 10:26 AM

Probably could have made 3x more but had no use for that many.


proly more like 10x more
then spread the love...

#19 Hippie3

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Posted 24 March 2005 - 10:27 AM

Can anyone tell my FOAF how to take better pictures?


i'd place a young attractive nekkid chick in each
holding the items to be highlighted...

#20 Hippie3

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Posted 24 March 2005 - 10:30 AM

keep gravity from pulling the spores to the walls of the syringes


i don't think gravity is the sole cause,
it does the settling part
but not the sticking.
i suspect part of the stickiness
is electrostatic attraction to the plastic sides,
as well as spores being slightly sticky anyway
esp. fresh wet spores.
i wonder if an ionizer in the area
might help reduce electrostatic attraction...




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