Paradox
©
Fisana

Jump to content


Photo
- - - - -

Crazy Agar Tek


  • Please log in to reply
6 replies to this topic

#1 crazy1

crazy1

    Celestial Traveler

  • VIP
  • 2,344 posts

Posted 28 June 2007 - 06:53 PM

CRAZY AGAR TEK

OK lets get going. First you need all the supplies
Agar Agar
Sugar Source
Nutritional Source
Nitrogen Source
20 Petri dishes

I used Agar Agar, Malt Barley Sugar Extract, Nutritional Yeast Flakes, and Peptone.
The Peptone is not necessary though.


P6280910-2.jpg

Now get a mason jar with 500ml of water

P6280911-2.jpg

Now add 1 Tablespoon and 1 teaspoon of Agar to the water


P6280913-2.jpg


P6280912-2.jpg

Do the same with the sugar source. 1 Tablespoon and 1 teaspoon
Now you can add a pinch of the nutritional source and the nitrogen source.
Shake it up and it will look like this.

 

P6280918-2.jpg

Now PC it @ 15PSI for 45 minutes.
Boil overs do happen


P6280925-2.jpg

Now pour your plates!!!!!!
Mine are poured about this deep. 1/2 way or about there.

 

P6280929-2.jpg

Now good luck to ya!!!!
 


Edited by Sidestreet, 20 August 2015 - 08:48 AM.

  • Boebs likes this

#2 the jesus

the jesus

    Mycotopiate

  • Expired Member
  • 2,583 posts

Posted 28 June 2007 - 06:58 PM

That is a nice writeup crazy, thanks for taking the time to put it together for us. Hero status for sure. :love::lol:

#3 Hippie3

Hippie3

    DUNG DEALER

  • Founders
  • 40,642 posts

Posted 28 June 2007 - 07:51 PM

no parafilm ?
what about stacking plates up high ?

#4 crazy1

crazy1

    Celestial Traveler

  • VIP
  • 2,344 posts

Posted 28 June 2007 - 07:57 PM

Well i don't put the parafilm on till I inoculate the plates. I let them dry out some first.
And have not had good luck stacking the plates up Hip. I don't wear gloves, wash in 71% alcohol every 15 min or so, and there is in no doubt skin particals getting in between the plates lids and bases. So I put all my plates in rows.

#5 Guest_greysRDbest_*

Guest_greysRDbest_*
  • Guest

Posted 28 June 2007 - 08:24 PM

heres a couple pics of him doing it

Attached Thumbnails

  • 05.jpg
  • 19.jpg


#6 siam_jim

siam_jim

    Mycotopia Mod

  • Expired Member
  • 1,853 posts

Posted 29 June 2007 - 10:05 AM

was wondering,,,even with the flowhood wouldn't it be better to start from the top row?... yes no?

man i love that FH.... ahhh thats "agar work"!!

siam

#7 crazy1

crazy1

    Celestial Traveler

  • VIP
  • 2,344 posts

Posted 29 June 2007 - 11:51 AM

Well the reason I start from the back is that any chance of contam, from me or the pouring vessel, will be behind the next row of plates I pour. So that way it doesn't blow into the plate. Hope that makes sense.
Thanx siam I love that hood to.




Like Mycotopia? Become a member today!