13 replies to this topic

[sadsoul]

Hi my friends!!!!!!!
I want to start playing with agar but i don't have the money for a real
flow hood filter , so i was thinking to do a small one just to transfer the
live tissue to the petri dish quickly , so i was searching for info and i found
a Hepa , Holmes replacement filter that removes up to 99.9% of airborne
particulates at .3 microns, should it work? And will a 135 c/f cage blower
work for this filter? Here's a pic ............//////////////////////

Best wishes !!!!!!!Always !!!!!!!!!Sincerely ..............////////////////

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[highflyer]

Id bet that filter does not give the static pressure it runs at on the packaging since its a replacement filter for a prebuilt unit. Read my post here on how to match a filter to a blower.
http://mycotopia.net...html?1069556827

Aside from that you would probably be better off working in a glovebox than trying to build a small flowhood from that filter. It wont create a laminar flow.

[destroy_erase_improve]

In fact, a properly made glove box will perform as well as a laminar flow hood. The flow hood is just much easier and faster to work with.
RR

that is the comment of the day imo
it gives hope to those who cant afford a flowhood but want to do agar and g2g transfers. if they dont mind the pain in the ass of working in a confined area, etc. it can be done.
comment of the fricken day

[highflyer]

I would definately agree. I only used a glovebox for several years before I made my first flowhood. They can easily get the job done. Its just a PITA compared to a hood.

[destroy_erase_improve]

if your doing agar dishes in a glovebox. are you moving slow as to not stir up any would be contams. or are you moving fast to elimate the amount of time the dishes are open

[sadsoul]

Thanks guys !!!!!!!!!
So yestertday i turned my ion generator all the way , sprayed the room with a 10% solution of bleach and no air movement , and i made a cardboard culture just to test my inviorment with some inner GT live tissue , Lets hope for the best ..........//////////////............

best wishes !!!!!!!!!!!!!!!!!!!!!!!Thanks again my friends !!!!!!!!!!!!!!!

[destroy_erase_improve]

and if i remember correctly you use soap and water to clean
not lysol or alky
do you know of a place to get the tyvek sleeves at a reasonable price by any chance?

[sadsoul]

It's wet , not dripping water but wet.
best wishes..............////////////

[destroy_erase_improve]

good luck sad
hope the ion works out for you
i have high hopes for it.

[sadsoul]

Thanks man !!
I hope too......////////......

[Hippie3]

Id bet that filter does not give the static pressure it runs at on the packaging since its a replacement filter for a prebuilt unit. Read my post here on how to match a filter to a blower.
http://mycotopia.net...html?1069556827
Aside from that you would probably be better off working in a glovebox than trying to build a small flowhood from that filter. It wont create a laminar flow.

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