Posted 30 March 2005 - 09:13 PM
I also heard you can use a blender and suck up the solution in a syringe.
I will also be doing Karo. Please if you can help me on this it would be Appreciated.
Posted 30 March 2005 - 09:57 PM
I am not the worlds expert on this but I do have one sucess under my belt with Karo Cloning so I will tell you how I did it.
1) PC'd 4 ml of Karo in 100 ml of distilled water in little jars. Tried tap and it always goes yellow. Distilled is good. I did 6 jars, three for Boomer 1 and three for Boomer 2.
2) wearing gloves and working in the flow hood, I flame starilized a pair of tweezers. Split the stem and tweezed in a pristine part of the untouched flesh to get a little chunk. Open jar quickly, put chunk in, close jar. Flame starilze the tweezers again and repeated 2 more time for Boomer 1 and three more times for boomer 2.
3) waited for 3 weeks and the mycelia in all six jars grew into white blobby clouds.
4) again with gloves and sterile syringes, open a jar and using a syringe, suck and blow the mycelia into little peices about 3 to 4 times then pull 12 ccs into the syringe and cap the jar. Label the syringe. Repeat for each of the jars. Put all jars in the fridge.
5) make 6 brf standard jars. Innoc each using 2-3ml from each syringe, one jar per syringe to test if it works or is contammed.
6) wait 3-4 weeks. Toss the jars that go green (went green in 3 days if at all). Wait for pins like in the following picture.
Now you should have a good idea of which jars were successful and which to toss. :)
Glove box should work nearly as well as the flowhood if you are careful. Good luck.
Posted 30 March 2005 - 10:44 PM
If you clone very large fruits from a small flush that is the genetics you'll get. That's not always a bad thing. I've seen huge flushes of little bitty mushies that really didn't add up to much, and I've seen three or four HUGE mushies on a casing, that easily would be as much product as a normal flush.