Helllllllooooo....i was looking over the cloning vaults and stumbled accross this thread
Mycotopia Web Archive: CloningUsing Dry Tissue
i'm so excited that i can clone from dried mushrooms because i had some a while back that were absolutely killer (not sure the strain) that im definately going to clone....which part of the dried mushroom do i have to use? or can i use any part of the mushroom? i'll probably use agar to grow myceilum and then transfer to rye
thanks!
cloning dried mushrooms
Started By
ttam
, Jul 30 2007 02:07 AM
18 replies to this topic
#2
Guest_vinz_*
Guest_vinz_*
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- Guest
Posted 30 July 2007 - 02:32 AM
try cloning the inner tissue of the mushroom
keep your agar mix a little on the wet side so it would hydrate the tissue
mold and bacteria would normally grow faster than the dried tissue.. so as soon as you see myc growth, transfer it right away to another dish!
good luck!
keep us posted with pictures ;)
keep your agar mix a little on the wet side so it would hydrate the tissue
mold and bacteria would normally grow faster than the dried tissue.. so as soon as you see myc growth, transfer it right away to another dish!
good luck!
keep us posted with pictures ;)
#3
ttam
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- Expired Member
- 86 posts
Mycophiliac
Posted 30 July 2007 - 02:44 AM
wow that sounds great! i can't wait to do it!
k so i've read the archives and i think i've got all the info i need on cloning...tell me if my summary is correct, just for clarification...so in summary i just take the tissue from the middle of the shroom, drop it onto the agar, put that petri dish into an incubator, transfer the myc to other agar quickly before contams get in, and finally transfer the good myc into a jar with PC'd grain?
thanks!
k so i've read the archives and i think i've got all the info i need on cloning...tell me if my summary is correct, just for clarification...so in summary i just take the tissue from the middle of the shroom, drop it onto the agar, put that petri dish into an incubator, transfer the myc to other agar quickly before contams get in, and finally transfer the good myc into a jar with PC'd grain?
thanks!
#4
Guest_vinz_*
Guest_vinz_*
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- Guest
Posted 30 July 2007 - 02:51 AM
yup, thats about it.. but you might need to do a lot of cleaning..
you can skip the incubator part and just put it in a small cardboard container or a box.. something clean.. dont forget the parafilm..
i keep mine in room temp but our room temp here is about 28-30C
gluck!
you can skip the incubator part and just put it in a small cardboard container or a box.. something clean.. dont forget the parafilm..
i keep mine in room temp but our room temp here is about 28-30C
gluck!
#6
goldenteacher1163
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- Free Member
- 322 posts
myco guru
Posted 30 July 2007 - 06:33 AM
can't wait to see results. This has always intrigued me
#7
WHITE RABBIT
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- Free Member
- 275 posts
Let's take a trip
Posted 02 August 2007 - 07:27 PM
I have some dried babys that I think I'll try this on too. Cool!
#8
joystik
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Mycotopiate
Posted 02 August 2007 - 07:55 PM
I will definitely be following this thread.
#9
jdl711
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P.L.U.R.
Posted 03 August 2007 - 03:17 PM
i never thought you could as the tissue is dead and carrys no oxygen when dried out, if it works i will be happily suprised.:amazed:
#10
Telepathic
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- Expired Member
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Spiritualized
Posted 03 August 2007 - 04:03 PM
I have a similar situation, ran across some extreme mushy a week back, let me know how it goes maybe ill try this out too.
#11
ttam
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- Expired Member
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Mycophiliac
Posted 03 August 2007 - 04:09 PM
well to be honest it might not be for a little while, but be assured i will keep everyone posted :D im just waitin on agar, petri dishes and a few other things and then i'll start clonin
#12
Telepathic
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- Expired Member
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Spiritualized
Posted 03 August 2007 - 04:15 PM
I too have no agar or petri dishes. I am almost a complete noob to the physical aspect of growing, though I have been reading up on it for almost a year.
#13
mycot
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- Expired Member
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Mycotopiate
Posted 03 August 2007 - 08:10 PM
I'd like to see a lot more work done in this area.(cloning dry tissue that is)
Perhaps we can improve on the Teks that we have presently got.
Perhaps we can improve on the Teks that we have presently got.
#14
fedshtkpndrk
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Prodigal Son
Posted 03 August 2007 - 08:30 PM
I will be watching this thread.
#15
Guest_Doesntmatter_*
Guest_Doesntmatter_*
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- Guest
Posted 03 August 2007 - 11:11 PM
my thoughts on dry tissue is that if at least a small amount of water is
still in the cells (vacuoles), the cell is still viable. If there is no water
left, call it dust and move on. if you can bend the fruit even the smallest
amount, you can clone it.
-DM-
still in the cells (vacuoles), the cell is still viable. If there is no water
left, call it dust and move on. if you can bend the fruit even the smallest
amount, you can clone it.
-DM-
#17
golly
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- Expired Member
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modapotato
Posted 04 August 2007 - 08:09 AM
The tissue can be as dry as a bone and still be re-animated..Best to make a bunch of plates, as the success rate is much lower than fresh..
#18
mycot
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- Expired Member
- 376 posts
Mycotopiate
Posted 07 August 2007 - 11:40 PM
Thanks for posting the links Hip.:thumbup: That second link I was unfamiliar with.
Nice tek Fahster.:bow:
Nice tek Fahster.:bow:
#19
Set
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- Banned Member
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Former Member
Posted 08 August 2007 - 08:38 AM
I've often wondered if you could resurrect old tissue in this manner. It's cool to see that it can be done!
