cloning dried mushrooms
Posted 30 July 2007 - 02:07 AM
Mycotopia Web Archive: CloningUsing Dry Tissue
i'm so excited that i can clone from dried mushrooms because i had some a while back that were absolutely killer (not sure the strain) that im definately going to clone....which part of the dried mushroom do i have to use? or can i use any part of the mushroom? i'll probably use agar to grow myceilum and then transfer to rye
Posted 30 July 2007 - 02:32 AM
keep your agar mix a little on the wet side so it would hydrate the tissue
mold and bacteria would normally grow faster than the dried tissue.. so as soon as you see myc growth, transfer it right away to another dish!
keep us posted with pictures ;)
Posted 30 July 2007 - 02:44 AM
k so i've read the archives and i think i've got all the info i need on cloning...tell me if my summary is correct, just for clarification...so in summary i just take the tissue from the middle of the shroom, drop it onto the agar, put that petri dish into an incubator, transfer the myc to other agar quickly before contams get in, and finally transfer the good myc into a jar with PC'd grain?
Posted 30 July 2007 - 02:51 AM
you can skip the incubator part and just put it in a small cardboard container or a box.. something clean.. dont forget the parafilm..
i keep mine in room temp but our room temp here is about 28-30C
Posted 02 August 2007 - 07:27 PM
Posted 03 August 2007 - 03:17 PM
Posted 03 August 2007 - 04:03 PM
Posted 03 August 2007 - 04:09 PM
Posted 03 August 2007 - 04:15 PM
Posted 03 August 2007 - 08:10 PM
Perhaps we can improve on the Teks that we have presently got.
Posted 03 August 2007 - 11:11 PM
still in the cells (vacuoles), the cell is still viable. If there is no water
left, call it dust and move on. if you can bend the fruit even the smallest
amount, you can clone it.
Posted 04 August 2007 - 08:09 AM
Posted 07 August 2007 - 11:40 PM
Nice tek Fahster.:bow:
Posted 08 August 2007 - 08:38 AM