getting ready to grow
Posted 02 August 2007 - 09:09 AM
Since I will have I think 4 syringes extra after shooting up the 12 jars, (sound right?) I may go ahead and order myco bags with ports if my assumption about them is correct.
Are these bags complete invitro tek solutions as well? Just inoculate and let them go right through to fruiting, or do you have to birth and case once colonized?
Please point me to the right thread if I missed it, I have been looking but am a bit unclear on the bags.
Posted 02 August 2007 - 09:56 AM
if you order empty bags and load them yourself.. you can do it invitro ala :
or if you order 'spawn bags' those would most probably have grain in them and are normally used for spawn..
or you can check out www.mycrotopia.com
hippie's site.. he has several sandman style bags that would go invitro or birth in the bag as is..
hippie has some grain (spawn bags) too
good luck :)
Posted 02 August 2007 - 01:48 PM
Posted 02 August 2007 - 07:26 PM
Posted 02 August 2007 - 08:40 PM
Posted 03 August 2007 - 08:52 AM
Posted 03 August 2007 - 09:11 AM
Are these bags complete invitro tek solutions as well? Just inoculate and let them go right through to fruiting , or do you have to birth and case once colonized?
or used as spawn.
Posted 03 August 2007 - 12:12 PM
1. The jars are in a state currently where contams are not a problem due to the vermiculite barrier?
2. after spore injection, how much water should I add and how/where?
3. after that (spores and water) should I seal up the four holes with tape or silicone, or is it ok just to leave it like that (assuming the vermiculite acts as an effective barrier and allows enough air exchange)
My spores should arrive anyday and we are off to the races...
this is the most fun i've had in years!
Posted 03 August 2007 - 12:18 PM
2. only water to be added is that which comprizes your spore solution
3. just leave the holes open, the verm will do the job
Posted 06 August 2007 - 01:12 PM
Posted 07 August 2007 - 07:41 AM
I moved everything home into my cellar. I think my concealment will be ok there. I used 2 syringes on 12 jars, 6 of one strain (south american cubies) and six another(MR cubie). I have them in a plastic drawer about 12x24x8 (They get plenty of light) with papertowels and a board covering. (this will allow for ventilation and let some of the heat/humidity out) I am using a submerged terrarium heater, which seems harmless enough and the temp is staying constant around 80f.
I innoculated using a lighter and heated up the needle after each jar till at least a portion of it glowed red. I found you have to boil out some of the spore solution after each injeciton. Then I wiped the needle with a sterile pad alcohol wipe, which I changed every three jars or so. I wore a sterile mask and washed my hands with antibacterial soap first. (btw i did the innoculation in my office which i think has a much lower prob of free floating contams than my home. at home there are 8 people, one of which is elderly and sick and 2 of which have a fairly severe mental handicap, plus 2 dogs so, its germ heaven)
The only real concern I have now is it is very humid there. I did not count on that. I am also using the submerged heater which adds to the humidity. SInce the jars are sealed and under the vermiculite barrier, as long as i innoculated ok, i think i am ok.
Now its the chicken sitting on the eggs thing.
Send me some patience
or send me some speed
or grow me those mushrooms
cause they're just what I need.
Any thoughts, thx
Posted 07 August 2007 - 01:54 PM
Posted 07 August 2007 - 08:44 PM
Posted 07 August 2007 - 08:53 PM
keep us posted.. those upside down jars you have on that wooden rack could be used for grain jars :thumbup:
dont introduce light just yet.. for invitro you can let light in early but not just yet coz no myc yet hehe
maybe when its 80%+
what strains did you work with btw?
Posted 08 August 2007 - 06:19 AM
The strains are all cubies. I know one was south American. the other was labeled MR, I checked my order and I am not really sure what this abbreviation stands for. I am going to research this on the spore vendor site and I will post it later today.
Posted 08 August 2007 - 06:21 AM
MR maybe matias romero
Posted 08 August 2007 - 12:06 PM
Posted 09 August 2007 - 07:50 PM
Man, I'm all excited.
Posted 10 August 2007 - 08:10 AM
Posted 10 August 2007 - 08:12 AM
Yes it's true!:headbang: After only three days there is trace mycelium in most of my jars. I thought at first the jars were dirty, but then in some pockets I saw very definite spider web type mold starting and in one, a nice white growth spot about the size of a pea. :eusa_clap (yeah, I look at them daily, only once, to make sure the temp is ok, heh)
Man, I'm all excited.