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Ps. azurescens and Ps. cyanescens multiple attempts


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#1 mycobri

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Posted 17 August 2007 - 07:11 PM

it is late in the season to start
however a dozen 1/2 pints of brf/verm
were started.


so far the azures have colonized faster than the wavy caps.
Six jars have colonized nicely and might finish.
5 or 6 more will be started shortly.

ps cy.jpg
The cyanescens wavy caps are colonizing a little slower. 5 jars are doing okay, but not as fast as the azures.
:weedpoke:
4 more 1/2 pt.s of ps. cyans will be started asap.

the goal is

• to spawn the cakes to hickory chips, with some potting soil and a few spoonfuls of rgs
in filter patch myco-bags

• to spawn some cakes into plastic trays with hickory chips and a wet cardboard bottom layer, covered with foil and breather holes to recover.

Then
• to spawn mycelium blocks to outdoor tubs with more wood chips, soil and some grass seed



i think it is very late to do so
but hoping the Summer will hang around longer
Fall will last until December
and Winter almost makes it to April

it is worth a try ;)

Attached Thumbnails

  • az bottoms.jpg
  • az 5 .jpg

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#2 CoyoteMesc

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Posted 17 August 2007 - 07:15 PM

They look good, should be a fun thread
and a great attempt, :thumbup:

#3 the_chosen_one

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Posted 17 August 2007 - 10:39 PM

i bet they'll be fine if your weather is anything like here. i usually don't start my azure projects until around august and they do just fine. and that's with outdoor beds. tubs should even be easier. definitely worth a try. :D

#4 mycobri

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Posted 30 August 2007 - 07:37 PM

thanks

an update.

the azures are really almost fully colonized.
ps az side.jpg
ps az bottom.jpg
the wavy cap cyans are slow but not stalled.
ps cy update side.jpg
ps cy update bottom.jpg

so far only the azure LC has shown growth.

here is the incubator -
room temp box.jpg
a cardboard box with a loose bag
at room temps about 70 F

so the next step would be to sterilize some hickory chips mixed with potting soil and a few spoonfuls of RGS
Then Spawn the finished azure myc to the filter patch bags for more incubation.

any tips or suggestions would be appreciated :bow:

#5 mycobri

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Posted 09 September 2007 - 07:54 PM

All 6 the Azure jars are now fully colonized :amazed:

Also the Azure LC is showing a few nice clusters of growth.

The ps. Cyans are about 80% colonized.

The next step will be making 6 filter-patch bags
using Waylit's woodlover mycobag tek http://mycotopia.net...n-mycobags.html

All the same ingredients will be used except the brand of cactus soil.
the same cactus soil cannot be found so the soil mix will be sifted peat moss, bone meal, bloodmeal & cottonseed meal in small amounts and a small amount of coir.

does this sound nutritious enough to fill in the gaps between the hickory chips?

worm castings are not available at this time. normal potting soil is another option for the mix:eusa_thin

opinions and tips are welcomed :)

#6 cheshire

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Posted 10 September 2007 - 05:25 PM

good luck :meditate:

#7 jimbojones

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Posted 10 September 2007 - 05:39 PM

I was thinking of doing outdoor blocks or bags. Since it is so late in the season and a patch takes time to colonize could one not just place colonized wood lover cakes or blocks in the suitable environment?

#8 Myc

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Posted 10 September 2007 - 06:49 PM

Nice work Mycobri!

I have studied these species pretty heavily this year and have had similar results to yours. The Azures are very aggressive and seem like they would rapidly colonize a brick! LOL
On the other hand, the Cyans were slow to respond to all efforts.
I've never used filter patch bags so I can't advise there but your strategy appears to be a sound one. Workman weighed in on one of my posts and gave me some information regarding the "island effect" which may help your spawning strategy when moving outdoors.

http://mycotopia.net...ice-please.html

I ordered the potting soil mentioned in Waylit's tek and it was quite expensive to ship it here. Upon examination it looks like your substitution will hold up well. Mix in bone meal @ about 4 tbsp/ 2 cubic foot of soil (or slightly less) and consult the directions for usage on the other nutrients. Might be able to use a little h-poo or cow poo instead of worm castings?

The woodlovers need the microbial activity provided by the potting soil to fruit. Since someone asked I'll mention that they won't fruit from BRF cakes.

Crumbling the colonized cakes and spawning to bleach soaked (1.25 tbsp/ gal) wood chips worked very well for me. If you read the post I attatched you'll note that I had problems with trichoderma. This was a result of poor ventilation and excess moisture buildup. Your idea with the aluminum foil and ventilation holes should work fine with no trich problems. I put my colonies in a friggin' black trash bag w/ no holes thinking the lack of light and co2 buildup would help..........NOT!!

I guess where I'm going with this is that no sterilization should be necessary. The filter patch bags might even be overkill....but I'M NO EXPERT. Constult the masters on this idea first.

Last but not least, my cultures are kicking ass in the great outdoors with strong, aggressive rizomorphic growth evident in both colonies. Here's hoping that the temps drop soon so I can see some Flying Saucers and Wavy Caps!!

Good luck! :thumbup:

#9 mycobri

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Posted 10 September 2007 - 07:06 PM

thanks for the feedback

my cultures are kicking ass in the great outdoors with strong, aggressive rizomorphic growth evident in both colonies. Here's hoping that the temps drop soon so I can see some Flying Saucers and Wavy Caps!!

way to go myc :thumbup:

#10 mycobri

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Posted 18 September 2007 - 12:55 AM

okay the Azures have moved to their new homes
2 jars were spawned per bag to 2 filt patch bags

which after 1 day is showing growth :eusa_pray
az bags.jpg
also one cake was used to spawn a small tray of webers hickory chips inside a tub
inside a shoebox for darkness

which also shows growth after one day:amazed:

the 6th cake was contaminated to the core
leaving only a greenish/black powder :puke:

Attached Thumbnails

  • az tray in box.jpg
  • az shoebox.jpg


#11 kocos

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Posted 18 September 2007 - 01:54 AM

I hate it when i find that, crack open a jar which looks completly good on the outside but when you try to dig out the contents it breaks apart and sends a cloud of nasty spores into the air.
The projects looking good man, is this going to be an indoor or semi indoor attempt?

#12 mycobri

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Posted 18 September 2007 - 07:21 AM

yeah Kocos that is exactly the contam.

this was going outside as logs of spawn in
cased woodchip bins - hopefully :)

an experimental indoor attempt could be fun though.
maybe if the small tray finishes
it can be cased and left in the fridge
or near an open window

#13 mycobri

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Posted 08 October 2007 - 11:58 PM

maybe not so late an attempt as the weather
has been incredibly warm :flamer:


the azures are aggressive and have all been
planted as big planks in flower pots outside
as suggested by waylitjim

these blocks were created by mixing the cakes with woodchips and leaving
to recover for two weeks
.azuresminiblocks.jpg

all the pots are doing well and i hope to see some woodlover fruits for the first time :amazed:

the ps. azurescens LC was also aggressive
and was partially drained and used to knoc
a few QTs of popcorn and soaked coffee stirrers
az lc wood corn.jpg

the more woodloving Myc the merrier
and the better my chances are of experiencing them :D

#14 Dave_the_stoner

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Posted 09 October 2007 - 12:20 AM

Your timing may be about right depending on your climate. I've been searching high, low, near and far all over the puget sound area, and have found none yet. But they are coming soon.

#15 lovesshrooms

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Posted 09 October 2007 - 06:04 AM

Good luck, im looking forward to seeing your results as azurescens are really quite pretty looking shrooms :)

#16 dpwutz

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Posted 13 October 2007 - 11:18 PM

i too am tryin the woodlovers this year. jars are starting. My question is: here in GA the temps outside should b good for fruting in the winter. I will be using tubs to start outside, but if i permantly mix them in the bed outside, will the 100 + temps in the summer kill the spores while dormant?

#17 dpwutz

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Posted 13 October 2007 - 11:43 PM

also, how long for the mycelium to show, ive heard it may take a few weeks.

#18 Jake420

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Posted 14 October 2007 - 01:08 AM

Temps over a hundred are a concern for spores and mycelium both, but to a lesser degree with the spores. Spores can last a bit through extreme heat. The mycelium on the other hand is very sensitive to excessive heat. This can be negated with plenty of shade and sprinkling/misting (misting hose is great for this) during the hottest hours of the day.

#19 mycobri

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Posted 25 October 2007 - 11:11 PM

here are some of the outdoor pots
the surface is kept slightly moist and the myc is poking through
underneath the surface lies thick white myc chunks :amazed:
az planted.jpg

here was the Azurescens LC beginning
on the popcorn - coffee stirrers jars.
az lc start.jpg

all the jars colonized nice and quick.
azcornrgsbrf.jpg

the LC has also recovered,
and the last bit will be used to knoc up more jars.
better late than never
azlcrecovering.jpg

some of the spawn from the LC
was spawned to these hickory woodchips for smoking food.
azlcwoodchips.jpg

these three buckets are in a race..
az race.jpg
RGS VS popcorn / woodsticks VS brf/verm cake spawn

there are still a few more fully colonized jars lying around.
azfullcolon.jpg



being that there is wood already attached to the popcorn
a few indoor fruiting experiments were made;

here is invitro fridge jar one.
azfridge1.jpg
jar two has woodchips sandwiching the spawn.
azfridge 2.jpg
they can go on the door of the fridge,
when it opens they will get a little fresh air and light/
or maybe they can just go in an outside shed :eusa_thin

also should they get a casing layer in these invitro jars?
i think they need one to fruit.

would it be possible to spawn directly to an outdoor bed of woodchips
and case right then and there?

any and all ideas and suggestions welcome.:hippie:
temps are finally dropping to 50's at night

#20 hyphaenation

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Posted 25 October 2007 - 11:29 PM

Looks good Mycobri !

I use smoking chips also. There is a brand or 2 of Alder that is sliver chips that are quite a bit finer than that hickory. Should work just fine though. Others have reported the addition of chopped straw and potting soil can be helpful. You can always some colonize and spawn it to another mix later.

Best of luck

cheers

H




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