11 replies to this topic

[wilgarn]

Im about to dunk my cakes after the first flush for 24 hours. I had a case of verticillum with it.

Will a bleach dip get rid of that after the dunk?

[dial8]

It should help a lot. Go with a 300:1 bleach:water ratio.

[automan]

It should help a lot. Go with a 300:1 bleach:water ratio.

i think you mean a 300:1 water:bleach ratio

[Mermaidia]

:lol: yeah...I'm sure that's what he meant. Thanks for catching that Auto. :bow: Can you imagine LOL

[wilgarn]

Cool, heres what I did :
1. 26 hour refridgerated dunk.
2. 15 minute 300:1 water:bleach unrefridgerated dunk.
3. Sprayed terrarium room really well with lysol.
4. Dumped old perlite. (it was 1.5" thick with a 1/4" to a 1/2" standing water...ewww I know bad)
5. Wiped terrarium with alcohol.
6. Applied DEC
7. Put 3/4" perlite in terrarium (no standing water)
8. increased the size of my polyfilled holes and added a fourth one (they're about 2 inches in diameter now).

Cant wait to see the results!

Thanks for the help guys :headbang:

[Defiance]

That sounds fine, keep us updated.
Good luck :thumbup:

[MrMycelium]

Sounds like you took every precaution, you should be fine. Cant wait to see the pics. :pirate:

[wilgarn]

Ok, heres an update for 5 days after the dunk/dip. Theres really nothing much to report. Definitely nothing picture worthy. The 3 cakes are developing a mild fuzz, seem to be darkened (brownish) and somewhat dry under the newly developing fuzz. (which there really isnt THAT much of--maybe 60% surface area covered)

Another event... I ordered 12 1/2 pint jars from mycotopia. 2 were broken upon arrival (damn post office, lol) which dropped it down to 10. I have a 1/2 bath that (sink and toilet only) that i cleaned top to bottom with bleach. It was the best I've ever cleaned anything because last time i innoculated stuff (4 bags and 6 jars) I lost all but 3 jars to contams. The syringes came from Sporeworks. So anyway, i checked the jars 5 days later and had lost 9 of them to 3 different contams. 6 had trich, 2 were completely colonized with what appered to be cobweb (although, strangely, the substrate was was almost black all the way through from this contam) and 1 was about 30% colonized with some weird light green (almost flourescent olive) contam growing down from the verm barrier. So out of 12, I have 1 that appears to be ok so far. It was the last one I innoculated. I know this because it was the only one that got injected in only 2 holes instead of 4 as I ran out of solution. So, out of 12 I got 1 :( .

So, i went ahead and cleaned the 9 contam'd jars really good and rinsed really thoroughly, made my own substrate and PC'd them. They've been sitting for 3 days now. Im gonna let em sit for about a week to make sure no contams emerge. If not, guess ill nocc them up. Man I'm just having so many problems with this. I really hope I at least get a good non-contam'd flush from my 3 good cakes...getting discouraged... Anyway gotta go to work, thanks for all you guys help.

BTW, I didnt mention names of companies i purchased from to put them down in anyway or anything, actually I mentioned them sort of to say "WTF, I know my sources are good, gonna have to examine my methods more closely to fix whatever the problem I'm having may be."

[BuckarooBanzai]

Two suggestions:

• You could have sterilized that Perlite instead of dumping it.
• Lysol is nasty and way less effective as a sterilant – I wouldn’t use it.

Drain that Perlite and cook it in the oven. 400F (almost dry) for 4 hours will kill anything and the Perl can easily deal with those temps. If ya got plenty, don’t worry about tossing it. Just dispose of it far away from your work area. 3 pounds of Perl in a dumpster looks kind of odd…

Lysol is a terrible air sanitizer and really bad for your pets and your mushrooms. Said another way: Lysol mutates mushrooms and can kill cats/birds/reptiles….and it is really shitty as an airborne sanitary agent! Oust is infinitely superior for sterilizing air spaces and common room surfaces. Bleach water is even better.

Other than that, sounds like ya got stuff rocking along. If you are dunking a case, it musta already done something good for ya….good luck and keep reporting!

[viewer6922]

I am not a pro by any means.....to knock up your jars,here is what I do. I wipe down my surface area with ISO Alcohol wipes( available at your local *ostco), Oust the heck out of the work area, not Lysol! Make sure your air conditioner is off first, that is, before starting injecting jars, or your clean up. Take a shower, Oust again, wipe down again,get a lighter, shot glass full of Iso Alcohol, place jars and syringes in front of you. Flame sterilize your needle, let that cool down, dip the needle in Iso Alcohol , do not let it touch anything for about a minute, inject one hole of the four, dip in Iso Alcohol again, inject next hole....repeat this as needed to complete your project. It is really common sense, keeping everything clean and sterile, including the air around you, the first of my two grows I used a homemade Glovebox,a lot of effort for really nothing, Just Keep Your Needle Sterile between injections! I now only use the glovebox for crucial lab experiments.

[wilgarn]

Little update, 13 days after the dunk. The vert is still there, but I managed to get my first fruit off it today. It appears to be touching the table, but its not... Should be an accurate weight. Picked off a coupla' small aborts too.

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[Hippie3]

finally got one, congrats, sounds like it hasn't been easy.
be careful when injecting not to dribble any spore solution
into the dry vermiculite layer that seals the lid holes.
the needle on the syringe must reach far enough to get
past the verm layer entirely
and not leak on the way back up/out.