psilocybe mexicana sclerotia-clone
Posted 05 July 2007 - 06:37 AM
1,Cook agar, pour out into plates, let cool, all done in GB.
2, Once cool, cut up sceletoria, remove iner tissue, dip into 3% peroxide, and transfer to plate. Seal with special tape i forgot the name of.
3, Incubate, transfer incase of contams.
So how does this sound? What are the chances? Any input is apretiated :)
peace pot and love
Posted 05 July 2007 - 11:57 AM
i broke the stone in half, don't cut it.. then i dug a chunk of it out of the center where its still sterile.. put maybe 3 or 4 peices per plate.
then just transfer when you see some good clean growth.
did that about 2 years ago.. and threw g2g i still got jars of stones going. actually i need to eat some of them, lol :)
Posted 05 July 2007 - 12:02 PM
Posted 05 July 2007 - 12:06 PM
Alternatively, you could attempt to grow a mushroom directly from the sclerotia by planting the sclerotia in a moist casing.
Posted 06 July 2007 - 01:41 AM
Posted 11 July 2007 - 07:01 AM
Posted 11 July 2007 - 07:14 AM
Cloning is getting living tissue to replicate. If it is dry it is dead.
Funny... People clone dry stuff all the time.
Posted 11 July 2007 - 07:27 AM
hope it turns out well for ya..
thinking you got that sclerotia from somewhere.. it might not be clean so getting the inside is a good idea :)
also, i suggest h202 agar..
iv read teks before in cloning dry mushroom tissue that they soak it in distilled water for a few days? not sure then dip it in h202 before placing it on agar..
Posted 11 July 2007 - 07:35 AM
Posted 11 July 2007 - 07:42 AM
i use glass dishes and reuse them all the time
if i reuse them, just wash then bleach dip for 10mins at least
and then pour h202 agar
if i reuse it for agar only and not peroxide agar, i wrap it in aluminum foil and PC it
thats a good investment :)
Posted 11 July 2007 - 07:54 AM
If it is dry it is dead.
Hey kokos, could you do some photography of the process and progress?
Posted 11 July 2007 - 07:59 AM
Posted 11 July 2007 - 08:25 AM
Posted 11 July 2007 - 02:22 PM
a weird dry mouth kinda after taste, i guess you could call it sour
personaly i just broke it up into pill sized chunks and swallowed with some koolaid
Posted 12 July 2007 - 01:24 AM
I wouldn't call it sour, more of an acrid aftertaste.
Ok well in that case i think iam dumping my sample. Upon ingesting a stone, the taste was normal, fungi like, but when it reached the back of my mouth the sour taste was overwhelming very much like when iam sick and i drink vitaminc cristals disolved in water. Perhaps someone spiked the stones with vitamin c? I might still give it a shot, you never know, but i think ill just clone somthing else thats dry for now and when i get my hands on some "clean" stones ill give this a shot. Thanx for the great input everyone, i will be doing this experiment sooner or later. Patience :)
Posted 12 July 2007 - 09:06 AM
Posted 13 July 2007 - 02:23 AM
You may have eaten a grain of grass seed also. They taste terrible and somewhat sour. They'll definitely give you the bitter beer face.
Could have been but it wasnt, it was small little brains we were eating, def not grains, so it must have been soaked in somthing.. oh well, looks like ill be getting a jalisco print soon. Ill make me own stones, dry one and clone that just for the fun of it :)
Posted 13 July 2007 - 11:08 AM