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Karo Tek


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#1 Toadstool

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Posted 12 October 2007 - 12:51 AM

I have done alot a looking around and can can not seem to make my way around to find what i am looking for. I was hopeing someone could show me or dirrect me where to go.

I am just looking for a simple Karo tek....
And also a tech for Lids for my Qt grain and also a resealable lid for my Karo jars.
I am a lil drunk so this will not make much sence so any help is wanted\
Thanks
Toad

#2 weed4all

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Posted 12 October 2007 - 01:00 AM

here ya go,

Mycotopia Web Archive: <b>No PC Honey/Karo Tek</b>

http://mycotopia.net...licon-lids.html

#3 losfreddy

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Posted 12 October 2007 - 11:05 AM

I belive for liquid culture., use a 4mm/cc s of Karo or honey per 100ml of water.. Can be pressure cooked or put in a tubberware, wrapped in plastic wrap and nuked for 5 minutes.. Inject spores and put in warm place to cultivate. *can't find the link right now.

#4 sandman

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Posted 12 October 2007 - 02:17 PM

Here are some files i found laying in a trash can that may be relevant
attached was this note:

1.Drill a hole in the plastic lid. Hole should be small enough for a tight fit so you can barely get the rubber stopper from a vacutainer or other test tube stopper to squeeze in.

001 drill hole.JPG

2. Use any kind of rubber stopper you can get. Look on ebay for real cheap expired vacutainers. All you need is the stopper.

002 vacutainer.JPG

3. Should have to use pliers to get the stopper in snug.

003 use pliers to pull.JPG

4. Here is the lid all ready to go. Just a plastic canning jar lid labeled "NOT FOR PROCESSING" on the box, but who cares about that. Available at any grocery store or wally world. I think there may be a source for pp5 deep lugged lids somewhere but i don't know I just use these. Works fine.

005 All your supplies.JPG

5. Gather your Karo light, measuring cup, quart jar, lid, magnetic stir bar, coffee filters and rubber bands.

004 finished lid.JPG

6. Pour the karo straight from the container into the empty jar.

006 pour the karo.JPG

7. Eyeball the amount of karo. Just cover the bottom.

007 eyeball the ammount.JPG

8. Measure 250 ml of water

008 250ml water.JPG

9. Pour the water into the jar

009 add water.JPG

10. Plop in your magnetic stir bar. You've got a magnetic stirrer right? Look on ebay. Don't forget the bars! The ability to stir 24/7 will reduce your colonization times.

010 add magnetic stir bar.JPG

11. Swirl

011 swirl.JPG

12. Put the lid on with just enough of a turn to where the lid doesn't fall off if you pick the jar up by the lid. Just barely one turn or so, you want the lid loose so gas can escape.

012 almost ready for nuke.JPG

13. Add 3 coffee filters and secure with rubber band over the loose lid.

013 coffee filter and rubber band.JPG

14. Put it in the nuker. I have a major big microwave so your times will vary.

014 ready for nuking.JPG

15. I like to use full power for the first blast to et it up to boiling for a minute.

015 slap it in.JPG

16. Which is like 2min30sec for me

016 full power 2 to 3 minutes.JPG

17. Then after that hit the microwave to half power for about 3 minutes

017 half power.JPG

18. After its done you must use a pot holder in one hand to hold the jar while you tighten the lid with your other hand through the coffee filter. Then remove from the microwave.

018 3 minutes.JPG

19. Then it should be nice and clear still.

019 finished and clear.JPG

20. Prepare a airport syringe which is just a syringe with polyfil stuffed in it where the plunger was. Wipe alcohol 70% with a cotton swab over the rubber stopper. Prick the airport into place. You will hear an immediate release of pressure if you listen closely. Leave the airport in for it to cool. Then remove.

019 finished and clear.JPG

21. Thats all. After its cool you are ready to roll. Inject spores through the rubber port after wiping with alcohol.

Attached Thumbnails

  • 020 airport pricking.JPG

  • Doctor D, GLP, MicroMycoMan and 1 other like this

#5 sandman

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Posted 12 October 2007 - 02:20 PM

Last one shows the airport in full, could only post 20 pics on a post. Would it be possible for some cool mod who knows how to put pics in ther place inside the post full size under the appropriate sentences do that for me?

Attached Thumbnails

  • 021 Jar with airport.JPG


#6 sandman

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Posted 13 October 2007 - 10:48 AM

just bumping this once before it fades into the dark abyss of old posts.

#7 lardnar

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Posted 13 October 2007 - 11:04 AM

well described there sandman with nice pictures

In my own experiments, I've come to like honey over karo and use 2cc of honey to 100ml of water instead of 4, the lower amount of sugar seems to make the mycelium grow a lot faster

#8 sandman

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Posted 13 October 2007 - 11:10 AM

Honey doesnt work for the microwave LC tek Ive listed here for some reason. It comes out totally cloudy and full of floaties. I made 4 karo jars and 2 honey jars and all the honey came out the same unusable...

As far as the ammount of sugars, i just eyeball it. The mycelium isnt too picky.

#9 lardnar

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Posted 13 October 2007 - 11:14 AM

Sorry there sandman, I've never tried the microwave I pressure cook em. Gotta be careful in the PC too, it's too easy to caramelize them
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#10 Toadstool

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Posted 17 October 2007 - 01:18 PM

Thanks guys! 1 more question what kinds of lids do yall use? I was thinking of just drilling hole in center and filling it with Poly and 1 small 1 on the side to inject and suck out with syringe(covered with tape unless in use). What ya think or is there an easier or better way I am all ears

#11 crazy1

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Posted 18 October 2007 - 06:55 AM

I use 5 ml of Karo to 100 ml water and just steam it for 20 min.
I like to use 1/2 pt jars (easier to get the myc with the syringe) with 2 holes covered with silicone. Then you can use Hips bubbler airport with it. I've had awesome success with this method.

#12 I_am_He

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Posted 08 November 2008 - 11:35 PM

I've never used any air exchange for liquid cultures and they grew just fine. Just one Hole in the lid off to the side. And as far as stirring, I just put some broken glass in the jar before it goes into the pc. don't have to spend any more money on bars. unless they're xannies

#13 sandman

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Posted 08 November 2008 - 11:42 PM

For what its worth, I don't do it this way no mo.

#14 NunYo

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Posted 08 November 2008 - 11:54 PM

I don't make any holes for air exchange on my LC containers either. They do fine. I also use the microwave method.

#15 Toadstool

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Posted 09 November 2008 - 02:23 AM

Hey Sand, how do you do it now? I have pretty much gave up on lc's. Can not get them to work for shit. I have tried cloning a lil tissue and spores from print and syringe.

#16 sandman

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Posted 09 November 2008 - 05:22 PM

I do diz http://mycotopia.net...ar-lids-mm.html

and diz http://www.shroomery...940/an/0/page/0

works so good it should be illegal.

#17 Gloid

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Posted 16 January 2009 - 05:37 PM

just a bump, 'cause this is a cool thread.:cool:




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