Mushroom cultures will grow old and stop producing good mushrooms when they are held and inoculated over and over on the same substrate. To prevent this from happening, you need only to change the substrate every other time. We do this by bouncing the culture back and forth using PDA and MEA agars. This keeps a good broad based spectrum of digestive enzymes, and keeps it from going weak. slp/fmrc
I have always wanted your opinion on this Steve, uh Mr. Peele; Do you think that substrate rotation is as big a consideration when using multispore innoculations? The infamous "PF" always said that one of his secrets was to NEVER rotate substrates, thus allowing his "strains" to further adjust to the PF method. That Yaj. fellow from a.d.m. always agreed. But the conventional myco-wisdom would sugest we proceed in a maner similar to what you have suggested. I think this is very important. I have yet to talk to ANYONE who has grown using a "closed loop" type of spore supply for long periods (5years+). There are a few anecdotal reports on the internet about potentcy losses with continued growing on BRF, but I put about as much faith in these as I do in the drunken incoherant ramblings of the neighborhood vagrents.
With states adopting antispore laws one after an other, I believe it is only a matter of time before the Federal Government gets involved and we all give this question some serious thinking.